Rhizobium binae
| Rhizobium binae | |
|---|---|
| Scientific classification | |
| Kingdom: | Bacteria |
| Phylum: | Proteobacteria |
| Class: | Alphaproteobacteria |
| Order: | Rhizobiales |
| Family: | Rhizobiaceae |
| Genus: | Rhizobium |
| Species: | R. binae |
| Binomial name | |
| Rhizobium binae Harun-or Rashid et al. 2015 | |
Description of Rhizobium binae (R)
Rhizobium binae (bi'nae. N.L. gen. fem. n. binae, abbreviation for Bangladesh Institute of Nuclear Agriculture, a research institute where the first steps to isolate the bacteria were taken) is soil inhabitant, Gram negative, aerobic, non-spore forming, rod shaped and plant root nodule forming bacteria. This species has been described by Harun-or Rashid et al (2015) from lentil root nodules in Bangladesh. Different housekeeping genes (recA, atpD, glnII) have 88 - 94% similarity with close relatives (Rhizobium etli and Rhizobum phaseoli) although the 16S rRNA gene has about 99% similarities. Type strain has 50 - 55% genetic relatedness with their close relatives in DNA-DNA hybridization experiment. Average nucleotide identity of whole genome sequences has 88- 92% similarities with close relatives. Colonies are circular, convex and creamy white on YEMA medium. Strains survive at pH values between 5.5 and 10 and tolerate 1 % NaCl in YEMA. They are very sensitive to ampicillin and resistant to kanamycin and nalidixic acid. Strains do not tolerate tetracycline and do not show any growth on LB medium.
Type strain is BLR195T (=LMG 28443T = DSM 29288T). The DNA G+C content of type strain is 61.5 %. Fatty acid composition of type strain is 15:0 iso 2-OH, 16:0, 16:0 3-OH, 18:0, 18:1ω9c, 18:0 3-OH, 18:1ω7c 11methyl, 19:0 cyclo ω8c, summed feature 2 and summed feature 8. Type strain could utilize dextrin, D-maltose, D-trehalose, D-cellobiose, gentiobiose, sucrose, D-raffinose, α-D-glucose, D-turanose, α-D lactose, D-fructose, D-melibiose, β-methyl-D-glucoside, salicin, N-acetyl-D-galactosamine, D-mannose, D-galactose, D-mannitol, D-sorrbitol, D-arabitol, glycerol, D-glucose-6-phosphate, D-fructose-6-phosphate, D-alanine, L-aspartic acid, L-histidine, l-pyroglutamic acid, quinic acid, D-saccharic acid, methyl pyruvate, L-lactic acid, citric acid, D-malic acid, L-malic acid, bromo-succinic acid, β-hydroxy-d,l-butyric acid and acetic acid. Type strain failed to utilize N-acetyle-D-mannosamine, 3-methyle glucose, inosine, glycyl-L-proline, L-arginine, D-galacturonic acid, D-glucuronic acid, glucuronamide, p-hydroxy-phenylacetic acid, D-lactic acid methyl ester, α-keto-glutaric acid, tween 40, propionic acid or formic acid.
Type strain could grow in the presence of lincomycin and potassium tellurite but not with 1% sodium lactate, troleandomycin, lithium chloride or sodium butyrate. Different gene sequences are available in NCBI and whole genome sequence in European nucleotide archive. Type strain was isolated from effective nodules of Lens culinaris from Feni district of Bangladesh. Different strains of this species can form effective nodules and enhance growth of lentil, peas and lathyrus, and are useful for Bio-fertilizer production.
References
1. Rashid, M.H., Young J.P.W., Everall, I., Clercx, P., Willems, A., Braun, M.S and Wink, W. (2015) Average nucleotide identity of genome sequences supports the description of Rhizobium lentis sp. nov., Rhizobium bangladeshense sp. nov. and Rhizobium binae sp. nov. from lentil (Lens culinaris) nodules. International Journal of Systematic and Evolutionary Microbiology, 65:3037-3045.
2. Rashid, M.H., Gonzalez, H., Young, J.P.W., and Wink, M. (2014) Rhizobium leguminosarum is the symbiont of lentil in the Middle East and Europe but not in Bangladesh. FEMS Microbiology Ecology, 87: 64 -77.
3. Rashid, M.H., Schafer, H., Gonzalez, H, and Wink, M. (2012) Genetic diversity of rhizobia nodulating lentil (Lens culinaris) in Bangladesh. Systematic and Applied Microbiology, 35: 98-109.