Rhizobium lentis
| Rhizobium lentis | |
|---|---|
| Scientific classification | |
| Kingdom: | Bacteria |
| Phylum: | Proteobacteria |
| Class: | Alphaproteobacteria |
| Order: | Rhizobiales |
| Family: | Rhizobiaceae |
| Genus: | Rhizobium |
| Species: | R. lentis |
| Binomial name | |
| Rhizobium lentis Harun-or Rashid et al. 2015 | |
Rhizobium lentis (len'tis. L. gen. n. lentis, referring to Lens, the plant genus from which the bacteria were isolated) is soil inhabitant, Gram negative, aerobic, non-spore forming, rod shaped and plant root nodule forming bacteria. This species has been described by Harun-or Rashid et al (2015) from lentil root nodules in Bangladesh. Different housekeeping genes (recA, atpD, glnII) have 90 - 95% similarity with close relatives (Rhizobium etli and Rhizobum phaseoli) although the 16S rRNA gene has about 99% similarities. Type strain has 50 - 60% genetic relatedness with their close relatives in DNA-DNA hybridization experiment. Average nucleotide identity of whole genome sequences has 88- 89% similarities with close relatives. Colonies are circular, convex and creamy white on YEMA medium. Strains grow at 12–37 °C but can survive at 4 °C. Strains grow well at pH 5.5 to 8.2 and are sensitive to 0.5 % NaCl in YEMA medium. Most of the strains are resistant to ampicillin, kanamycin and nalidixic acid. Strains do not tolerate tetracycline and do not show any growth on LB medium.
Type strain is BLR27T (= LMG 28441T = DSMZ 29286T). The DNA G+C content of type strain is 61.1%. The fatty acid composition of type strain is 15:0 iso 2-OH, 16:0, 16:0 3-OH, 18:0, 19:0 cyclo ω8c, summed featured 2, summed featured 3 and summed featured 8. Type strain could utilize α-D lactose, β-methyl-D-glucoside, D-sorbitol, D-mannito,D-arbitol-glycerol, D-fructose-6-phosphate, L-aspertic acid, D-gluconic acid, mucic acid, D-lactic acid methyl ester, L-lactic acid, L-histidine, L-lactic acid, β-hydroxy-D, L-butyric acid, D-malic acid, L-malic acid, acetic acid and formic acid. Type strain is unable to utilize D-maltose, D-trehalose, D-cellobiose, gentiobiose, sucrose, D-raffinose, α-D-glucose, D-turanose, D-melibose, mannose, galactose, 3-methyle glucose, inosine, D-aspertic acid, glycyl-L-proline, L-alanine, L-aspertic acid, L-arginine, L-serine, pectine, D-saccharic acid, p-hydroxy-phenylacetic acid, methyl pyruvate, citric acid, bromo-succinic acid, acetoacetic acid or propionic acid. Type strain could grow in the presence of lincomycin, tetrazolium violet and tetrazolium blue but not with 1% sodium lactate, troleandomycin, lithium chloride,potassium tellurite or sodium butyrate.
Different gene sequences are available in NCBI and whole genome sequence in European nucleotide archive. Type strain isolated from effective nodules of Lens culinaris from Natore district of Bangladesh. Different strains of this species can form nodules and enhance growth of lentil, peas and lathyrus.
References
1. Rashid, M.H., Young J.P.W., Everall, I., Clercx, P., Willems, A., Braun, M.S and Wink, W. (2015) Average nucleotide identity of genome sequences supports the description of Rhizobium lentis sp. nov., Rhizobium bangladeshense sp. nov. and Rhizobium binae sp. nov. from lentil (Lens culinaris) nodules. International Journal of Systematic and Evolutionary Microbiology, 65:3037-3045.
2. Rashid, M.H., Gonzalez, H., Young, J.P.W., and Wink, M. (2014) Rhizobium leguminosarum is the symbiont of lentil in the Middle East and Europe but not in Bangladesh. FEMS Microbiology Ecology, 87: 64 -77.
3. Rashid, M.H., Schafer, H., Gonzalez, H, and Wink, M. (2012) Genetic diversity of rhizobia nodulating lentil (Lens culinaris) in Bangladesh. Systematic and Applied Microbiology, 35: 98-109.