Ribosome shunting

Ribosome shunting is a mechanism of translation initiation in which ribosomes physically bypass, or shunt over, parts of the 5' untranslated region to reach the initiation codon. This enables viruses to have more information than usual in an mRNA molecule. Some viral RNAs have been shown to use ribosome shunting as a more efficient form of translation during certain stages of viral life cycle or when translation initiation factors are scarce (e.g. cleavage by viral proteases). Some viruses known to use this mechanism include adenovirus, Sendai virus, human papillomavirus, duck hepatitis B pararetrovirus, rice tungro bacilliform viruses, and cauliflower mosaic virus. In these viruses the ribosome is directly translocated from the upstream initiation complex to the start codon (AUG) without the need for eIF4A helices activity to unwind RNA secondary structures.[1]

Ribosome shunting in Cauliflower mosaic virus

Translation of Cauliflower mosaic virus (CaMV) 35S RNA is initiated by a ribosome shunt.[2] The 35S RNA of CaMV contains a ~600nt leader sequence which contains 7-9 short open reading frames (sORFs) depending on the strain. This long leader sequence has the potential to form an extensive complex stem-loop structure, which is an inhibitory element for expression of following ORFs. However, translation of ORFs downstream of the CaMV 35S RNA leader has been commonly observed.[3] Ribosome shunting model indicates with the collaboration of initiation factors, ribosomes start scanning from capped 5’-end and scans for a short distance until it hits the first sORF.[4] The hairpin structure formed by leader brings the first long ORF into the close spatial vicinity of a 5’-proximal sORF.[5] After read through sORF A, the 80S scanning ribosome disassembles at the stop codon, which is the shunt take-off site. The 40S ribosomal subunits keep combining with RNA, and bypass the strong stem-loop structural element, land at the shunt acceptor site, resume scanning and reinitiate at the first long ORF. 5’-proximal sORF A and the stem-loop structure itself are two essential elements for CaMV shunting [5]. sORFs with 2-15 codons, and 5-10 nts between sORF stop codon and the base of the stem structure are optimal for ribosome shunting, while the minimal (start-stop) ORF does not promote shunting.[6]

Ribosome shunting in Rice tungro bacilliform pararetrovirus

Ribosome shunting process was first discovered in CaMV in 1993, and then was reported in Rice tungro bacilliform virus (RTBV) at 1996.[7] The mechanism of ribosome shunting in RTBV resembles that in CaMV: it also requires the first short ORF as well as a following strong secondary structure. Swapping of the conserved shunt elements between CaMV and RTBV revealed the importance of nucleotide composition of the landing sequence for efficient shunting, indicating that the mechanism of ribosome shunting is evolutionary conserved in plant pararetroviruses.[8]

Ribosome shunting in Sendai virus

Sendai virus Y proteins are initiated by ribosome shunting. Among 8 primary translation products of Sendai virus P/C mRNA, leaky scanning accounts for translation of protein C’, P, and C proteins, while expression of protein Y1 and Y2 is initiated via a ribosomal shunt discontinuous scanning. Scanning complex enters 5’ cap and scan ~50 nt of 5’ UTR, and then is transferred to an acceptor site at or close the Y initiation codons. In the case of Sendai virus, no specific donor site sequences are required.[9][10]

Ribosome shunt in Adenovirus

Ribosome shunting is observed during expression of late adenovirus mRNAs. Late adenovirus mRNAs contains a 5’ tripartite leader, a highly conserved 200-nt NTR with a 25- to 44- nt unstructured 5’ conformation followed by a complex group of stable hairpin structure, which confers preferential translation by reducing the requirement for the eIF-4F (cap-binding protein complex), which is inactivated by adenovirus to interfere cellular protein translation. When eIF4E is abundant, both linear scanning and shunting are performed; however, when eIF4E is altered or deactivated during late adenovirus infection of heat shock, the tripartite leader exclusively and efficiently directs initiation by shunting.[11]

In the case of adenovirus late mRNA and hsp70 mRNA, instead of recognition of stop codon of first short ORF, pausing of translation is caused by scanning ribosome with three conserved sequences that are complementary to the 3’ hairpin of 18S ribosomal RNA.[12]

References

  1. ↑ Edgil, D; Polacek, C; Harris, E (2006). "Dengue virus utilizes a novel strategy for translation initiation when cap-dependent translation is inhibited". Journal of Virology 80 (6): 2976–86. doi:10.1128/JVI.80.6.2976-2986.2006. PMC 1395423. PMID 16501107.
  2. ↑ Fütterer, Johannes; Kiss-László, Zsuzsanna; Hohn, Thomas (1993). "Nonlinear ribosome migration on cauliflower mosaic virus 35S RNA". Cell 73 (4): 789–802. doi:10.1016/0092-8674(93)90257-Q. PMID 8500171.
  3. ↑ Dominguez, DI; Ryabova, LA; Pooggin, MM; Schmidt-Puchta, W; Fütterer, J; Hohn, T (1998). "Ribosome shunting in cauliflower mosaic virus. Identification of an essential and sufficient structural element". The Journal of Biological Chemistry 273 (6): 3669–78. doi:10.1074/jbc.273.6.3669. PMID 9452497.
  4. ↑ Ryabova, Lyubov A.; Pooggin, Mikhail M.; Hohn, Thomas (2006). "Translation reinitiation and leaky scanning in plant viruses". Virus Research 119 (1): 52–62. doi:10.1016/j.virusres.2005.10.017. PMID 16325949.
  5. ↑ Pooggin, MM; Fütterer, J; Skryabin, KG; Hohn, T (1999). "A short open reading frame terminating in front of a stable hairpin is the conserved feature in pregenomic RNA leaders of plant pararetroviruses". The Journal of general virology 80 (8): 2217–28. doi:10.1099/0022-1317-80-8-2217. PMID 10466822.
  6. ↑ Pooggin, MM; Hohn, T; Fütterer, J (2000). "Role of a short open reading frame in ribosome shunt on the cauliflower mosaic virus RNA leader". The Journal of Biological Chemistry 275 (23): 17288–96. doi:10.1074/jbc.M001143200. PMID 10747993.
  7. ↑ Fütterer, J; Potrykus, I; Bao, Y; Li, L; Burns, TM; Hull, R; Hohn, T (1996). "Position-dependent ATT initiation during plant pararetrovirus rice tungro bacilliform virus translation". Journal of Virology 70 (5): 2999–3010. PMC 190159. PMID 8627776.
  8. ↑ Pooggin, M. M.; Ryabova, LA; He, X; Fütterer, J; Hohn, T (2006). "Mechanism of ribosome shunting in Rice tungro bacilliform pararetrovirus". RNA 12 (5): 841–50. doi:10.1261/rna.2285806. PMC 1440904. PMID 16556934.
  9. ↑ De Breyne, S; Simonet, V; Pelet, T; Curran, J (2003). "Identification of a cis-acting element required for shunt-mediated translational initiation of the Sendai virus Y proteins". Nucleic Acids Research 31 (2): 608–18. doi:10.1093/nar/gkg143. PMC 140508. PMID 12527769.
  10. ↑ Latorre, P; Kolakofsky, D; Curran, J (1998). "Sendai virus Y proteins are initiated by a ribosomal shunt". Molecular and Cellular Biology 18 (9): 5021–31. doi:10.1128/mcb.18.9.5021. PMC 109087. PMID 9710586.
  11. ↑ Yueh, A; Schneider, RJ (1996). "Selective translation initiation by ribosome jumping in adenovirus-infected and heat-shocked cells". Genes & Development 10 (12): 1557–67. doi:10.1101/gad.10.12.1557. PMID 8666238.
  12. ↑ Yueh, A; Schneider, RJ (2000). "Translation by ribosome shunting on adenovirus and hsp70 mRNAs facilitated by complementarity to 18S rRNA". Genes & Development 14 (4): 414–21. PMC 316380. PMID 10691734.

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