SBP-tag

The Streptavidin-Binding Peptide (SBP)-Tag is a 38-amino acid sequence that may be engineered into recombinant proteins. Recombinant proteins containing the SBP-Tag bind to streptavidin and this property may be utilized in specific purification, detection or immobilization strategies.

The sequence of the SBP tag is MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP.[1]

Discovery

The Streptavidin-Binding Peptide was discovered within a library of seven trillion stochastically-generated peptides using the in vitro selection technique of mRNA Display. Selection was performed by incubating with streptavidin-agarose followed by elution with biotin.[2] The SBP-Tag has been shown to bind streptavidin with an equilibrium dissociation constant of 2.5nM[1][2] and is readily eluted with biotin under native conditions.[1][2]

Applications

Protein purification

Because of the mild elution conditions (biotin plus wash buffer) SBP-Tagged proteins can be generated in a relatively pure state with a single purification step.[1][3][4] There are several relatively abundant mammalian proteins that inherently associate with the IMAC matrices that bind to the more commonly used Polyhistidine-tag (His-tag). For this reason non-IMAC purification protocols, including with the SBP-Tag, are often preferred for proteins that are expressed in mammalian cells.

Protein complex purification

Complexes of interacting proteins may also be purified using the SBP-Tag because elution with biotin permits recovery under conditions in which desired complexes remain associated. For example, the Condensin Complex was purified by Kim et al. [2010] and complexes with the TAZ transcriptional co-activator were purified by Zhang et al. [2009]. The SBP-Tag has also been incorporated into several Tandem Affinity Purification (TAP) systems in which successive purification steps are utilized with multiple tags, for example GFP fusion proteins and BTK-protein complexes were purified using a TAP protocol with the SBP-Tag and the His-Tag,[5][6] HDGF-protein complexes were purified using a TAP protocol with the SBP-Tag and with the FLAG-tag[7] and Wnt complexes were purified using a TAP protocol with the SBP-Tag and with the [Calmodulin-Tag].[8] TAP is generally used with protein complexes and several studies report significant improvements in purity and yield when the SBP-Tag TAP systems are compared to non-SBP-Tag systems.[9][10][11] Commercial TAP systems that use the SBP-Tag include the Interplay® Adenoviral and Mammalian TAP Systems sold by Agilent Technologies, similar products are sold by Sigma-Aldrich.[12]

Proteomics

Screens for biologically relevant protein-protein interactions have been performed using Tandem Affinity Purification (TAP) with the SBP-Tag and Protein A,[10] for interaction proteomics and transcription factor complexes with the SBP-Tag and Protein G,[10][13] for proteins that interact with the Dengue Virus protein DENV-2 NS4A with the SBP-Tag and the Calmodulin Tag.[14] and for proteins that interact with protein phosphatase 2A (PP2A) with the SBP-Tag and the hemagglutinin (HA)-tag.[11]

Imaging

The SBP-Tag will also bind to streptavidin or streptavidin reagents in solution. Applications of these engineered associations include the visualization of specific proteins within living cells,[15] monitoring of the kinetics of the translation of individual proteins in an in vitro translation system,[16] control of the integration of a multi-spanning membrane protein into the endoplasmic reticulum by fusing the SBP-Tag to the N-terminal translocation sequence and then halting integration with streptavidin and restarting integration with biotin.[17][18] Fluorescent streptavidin reagents (e.g. streptavidin-HRP) can be used to visualize the SBP-tag by immunoblotting of SDS-PAGE.[1][19][20] Additionally, antibodies to the SBP-tag are available commercially.

Surface plasmon resonance

The SBP-Tag has been used to reversibly immobilize recombinant proteins onto streptavidin-functionalized surfaces thereby permitting interaction assessment such as by surface plasmon resonance (SPR) techniques with re-use of the functionalized surface.[21] SPR has also been used to compare the SBP-Tag with other streptavidin-binding peptides such as Strep-tag.[22]

See also

References

  1. 1 2 3 4 5 Keefe, Anthony D.; Wilson, David S.; Seelig, Burckhard; Szostak, Jack W. (2001). "One-Step Purification of Recombinant Proteins Using a Nanomolar-Affinity Streptavidin-Binding Peptide, the SBP-Tag". Protein Expression and Purification 23 (3): 440–6. doi:10.1006/prep.2001.1515. PMID 11722181.
  2. 1 2 3 Wilson, David S.; Keefe, Anthony D.; Szostak, Jack W. (2001). "The use of mRNA display to select high-affinity protein-binding peptides". Proceedings of the National Academy of Sciences 98 (7): 3750–5. doi:10.1073/pnas.061028198. PMC: 31124. PMID 11274392.
  3. ↑ Ichikawa, Muneyoshi; Watanabe, Yuta; Murayama, Takashi; Toyoshima, Yoko Yano (2011). "Recombinant human cytoplasmic dynein heavy chain 1 and 2: Observation of dynein-2 motor activity in vitro". FEBS Letters 585 (15): 2419–23. doi:10.1016/j.febslet.2011.06.026. PMID 21723285.
  4. ↑ Li, Feng; Herrera, Jeremy; Zhou, Sharleen; Maslov, Dmitri A.; Simpson, Larry (2011). "Trypanosome REH1 is an RNA helicase involved with the 3'-5' polarity of multiple gRNA-guided uridine insertion/deletion RNA editing". Proceedings of the National Academy of Sciences 108 (9): 3542–7. doi:10.1073/pnas.1014152108. PMC: 3048136. PMID 21321231.
  5. ↑ Li, Yifeng; Franklin, Sarah; Zhang, Michael J.; Vondriska, Thomas M. (2011). "Highly efficient purification of protein complexes from mammalian cells using a novel streptavidin-binding peptide and hexahistidine tandem tag system: Application to Bruton's tyrosine kinase". Protein Science 20 (1): 140–9. doi:10.1002/pro.546. PMC: 3047070. PMID 21080425.
  6. ↑ Kobayashi, Takuya; Morone, Nobuhiro; Kashiyama, Taku; Oyamada, Hideto; Kurebayashi, Nagomi; Murayama, Takashi (2008). Imhof, Axel, ed. "Engineering a Novel Multifunctional Green Fluorescent Protein Tag for a Wide Variety of Protein Research". PLoS ONE 3 (12): e3822. doi:10.1371/journal.pone.0003822. PMC: 2585475. PMID 19048102.
  7. ↑ Zhao, Jian; Yu, Hongxiu; Lin, Ling; Tu, Jun; Cai, Lili; Chen, Yanmei; Zhong, Fan; Lin, Chengzhao; et al. (2011). "Interactome study suggests multiple cellular functions of hepatoma-derived growth factor (HDGF)". Journal of Proteomics 75 (2): 588–602. doi:10.1016/j.jprot.2011.08.021. PMID 21907836.
  8. ↑ Ahlstrom, Robert; Yu, Alan S. L. (2009). "Characterization of the kinase activity of a WNK4 protein complex". AJP: Renal Physiology 297 (3): F685–92. doi:10.1152/ajprenal.00358.2009. PMC: 2739714. PMID 19587141.
  9. ↑ Kyriakakis, Phillip P.; Tipping, Marla; Abed, Louka; Veraksa, Alexey (2008). "Tandem affinity purification in Drosophila: The advantages of the GS-TAP system". Fly 2 (4): 229–35. PMID 18719405.
  10. 1 2 3 Bürckstümmer, Tilmann; Bennett, Keiryn L; Preradovic, Adrijana; Schütze, Gregor; Hantschel, Oliver; Superti-Furga, Giulio; Bauch, Angela (2006). "An efficient tandem affinity purification procedure for interaction proteomics in mammalian cells". Nature Methods 3 (12): 1013–9. doi:10.1038/nmeth968. PMID 17060908.
  11. 1 2 Glatter, Timo; Wepf, Alexander; Aebersold, Ruedi; Gstaiger, Matthias (2009). "An integrated workflow for charting the human interaction proteome: Insights into the PP2A system". Molecular Systems Biology 5 (1): 237. doi:10.1038/msb.2008.75. PMC: 2644174. PMID 19156129.
  12. ↑ Li, Yifeng (2011). "The tandem affinity purification technology: An overview". Biotechnology Letters 33 (8): 1487–99. doi:10.1007/s10529-011-0592-x. PMID 21424840.
  13. ↑ Van Leene, Jelle; Eeckhout, Dominique; Persiau, Geert; Van De Slijke, Eveline; Geerinck, Jan; Van Isterdael, Gert; Witters, Erwin; De Jaeger, Geert (2011). "Isolation of Transcription Factor Complexes from Arabidopsis Cell Suspension Cultures by Tandem Affinity Purification". In Yuan, Ling; Perry, Sharyn E. Plant Transcription Factors. Methods in Molecular Biology 754. pp. 195–218. doi:10.1007/978-1-61779-154-3_11. ISBN 978-1-61779-153-6. PMID 21720954.
  14. ↑ Anwar, Azlinda; Leong, K. M.; Ng, Mary L.; Chu, Justin J. H.; Garcia-Blanco, Mariano A. (2009). "The Polypyrimidine Tract-binding Protein Is Required for Efficient Dengue Virus Propagation and Associates with the Viral Replication Machinery". Journal of Biological Chemistry 284 (25): 17021–9. doi:10.1074/jbc.M109.006239. PMC: 2719340. PMID 19380576.
  15. ↑ McCann, Corey M.; Bareyre, Florence M.; Lichtman, Jeff W.; Sanes, Joshua R. (2005). "Peptide tags for labeling membrane proteins in live cells with multiple fluorophores". BioTechniques 38 (6): 945–52. doi:10.2144/05386IT02. PMID 16018556.
  16. ↑ Takahashi, Shuntaro; Iida, Masaaki; Furusawa, Hiroyuki; Shimizu, Yoshihiro; Ueda, Takuya; Okahata, Yoshio (2009). "Real-Time Monitoring of Cell-Free Translation on a Quartz-Crystal Microbalance". Journal of the American Chemical Society 131 (26): 9326–32. doi:10.1021/ja9019947. PMID 19518055.
  17. ↑ Kida, Yuichiro; Morimoto, Fumiko; Sakaguchi, Masao (2007). "Two translocating hydrophilic segments of a nascent chain span the ER membrane during multispanning protein topogenesis". The Journal of Cell Biology 179 (7): 1441–52. doi:10.1083/jcb.200707050. PMC: 2373506. PMID 18166653.
  18. ↑ Kida, Y.; Morimoto, F.; Sakaguchi, M. (2008). "Signal Anchor Sequence Provides Motive Force for Polypeptide Chain Translocation through the Endoplasmic Reticulum Membrane". Journal of Biological Chemistry 284 (5): 2861–6. doi:10.1074/jbc.M808020200. PMID 19010775.
  19. ↑ Edelmann, Mariola J.; Iphöfer, Alexander; Akutsu, Masato; Altun, Mikael; Di Gleria, Katalin; Kramer, Holger B.; Fiebiger, Edda; Dhe-Paganon, Sirano; Kessler, Benedikt M. (2009). "Structural basis and specificity of human otubain 1-mediated deubiquitination". Biochemical Journal 418 (2): 379–90. doi:10.1042/BJ20081318. PMID 18954305.
  20. ↑ Hoer, Simon; Smith, Lorraine; Lehner, Paul J. (2007). "MARCH-IX mediates ubiquitination and downregulation of ICAM-1". FEBS Letters 581 (1): 45–51. doi:10.1016/j.febslet.2006.11.075. PMID 17174307.
  21. ↑ Li, Yong-Jin; Bi, Li-Jun; Zhang, Xian-En; Zhou, Ya-Feng; Zhang, Ji-Bin; Chen, Yuan-Yuan; Li, Wei; Zhang, Zhi-Ping (2006). "Reversible immobilization of proteins with streptavidin affinity tags on a surface plasmon resonance biosensor chip". Analytical and Bioanalytical Chemistry 386 (5): 1321–6. doi:10.1007/s00216-006-0794-6. PMID 17006676.
  22. ↑ Huang, Xu; Zhang, Xian-En; Zhou, Ya-Feng; Zhang, Zhi-Ping; Cass, Anthony E. G. (2007). "Construction of a high sensitive Escherichia coli alkaline phosphatase reporter system for screening affinity peptides". Journal of Biochemical and Biophysical Methods 70 (3): 435–9. doi:10.1016/j.jbbm.2006.10.006. PMID 17156847.

Further reading

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