SEC16B

SEC16 homolog B, endoplasmic reticulum export factor
Identifiers
Symbols	SEC16B ; LZTR2; PGPR-p117; RGPR; SEC16S
External IDs	OMIM: 612855 MGI: 2148802 HomoloGene: 13227 GeneCards: SEC16B Gene
Gene ontology Molecular function • protein binding Cellular component • Golgi membrane • endoplasmic reticulum membrane • intracellular membrane-bounded organelle Biological process • endoplasmic reticulum organization • peroxisome organization • positive regulation of gene expression • protein transport • peroxisome fission • COPII vesicle coating • positive regulation of protein exit from endoplasmic reticulum • protein localization to endoplasmic reticulum Sources: Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	89866	89867
Ensembl	ENSG00000120341	ENSMUSG00000026589
UniProt	Q96JE7	Q91XT4
RefSeq (mRNA)	NM_033127	NM_001159986
RefSeq (protein)	NP_149118	NP_001153458
Location (UCSC)	Chr 1: 177.92 – 177.98 Mb	Chr 1: 157.51 – 157.57 Mb
PubMed search

Protein transport protein Sec16B is a protein that in humans is encoded by the SEC16B gene.^[1][2][3]

Model organisms

Sec16b knockout mouse phenotype

Characteristic	Phenotype
Homozygote viability	Normal
Fertility	Normal
Body weight	Normal
Anxiety	Normal
Neurological assessment	Normal
Grip strength	Normal
Hot plate	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Body temperature	Normal
Eye morphology	Normal
Clinical chemistry	Abnormal[4]
Haematology	Normal
Micronucleus test	Normal
Heart weight	Normal
Salmonella infection	Normal[5]
Citrobacter infection	Normal[6]
All tests and analysis from[7][8]

Model organisms have been used in the study of SEC16B function. A conditional knockout mouse line, called Sec16b^{tm1a(KOMP)Wtsi[9][10]} was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists.^[11][12][13]

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.^[7][14] Twenty one tests were carried out on mutant mice and one significant abnormality was observed: homozygote mutants had decreased circulating cholesterol levels.^[7]

References

1. Nagase T, Kikuno R, Ohara O (Sep 2001). "Prediction of the coding sequences of unidentified human genes. XXI. The complete sequences of 60 new cDNA clones from brain which code for large proteins". DNA Res 8 (4): 179–87. doi:10.1093/dnares/8.4.179. PMID 11572484.
2. Misawa H, Yamaguchi M (Oct 2001). "Molecular cloning and sequencing of the cDNA coding for a novel regucalcin gene promoter region-related protein in rat, mouse and human liver". Int J Mol Med 8 (5): 513–20. doi:10.3892/ijmm.8.5.513. PMID 11605020.
3. "Entrez Gene: LZTR2 leucine zipper transcription regulator 2".
4. "Clinical chemistry data for Sec16b". Wellcome Trust Sanger Institute.
5. "Salmonella infection data for Sec16b". Wellcome Trust Sanger Institute.
6. "Citrobacter infection data for Sec16b". Wellcome Trust Sanger Institute.
7. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.
8. Mouse Resources Portal, Wellcome Trust Sanger Institute.
9. "International Knockout Mouse Consortium".
10. "Mouse Genome Informatics".
11. Skarnes, W. C.; Rosen, B.; West, A. P.; Koutsourakis, M.; Bushell, W.; Iyer, V.; Mujica, A. O.; Thomas, M.; Harrow, J.; Cox, T.; Jackson, D.; Severin, J.; Biggs, P.; Fu, J.; Nefedov, M.; De Jong, P. J.; Stewart, A. F.; Bradley, A. (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature 474 (7351): 337–342. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
12. Dolgin E (2011). "Mouse library set to be knockout". Nature 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
13. Collins FS, Rossant J, Wurst W (2007). "A Mouse for All Reasons". Cell 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
14. van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism.". Genome Biol 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.

Further reading

• Bhattacharyya D, Glick BS (2007). "Two Mammalian Sec16 Homologues Have Nonredundant Functions in Endoplasmic Reticulum (ER) Export and Transitional ER Organization". Mol. Biol. Cell 18 (3): 839–49. doi:10.1091/mbc.E06-08-0707. PMC 1805085. PMID 17192411.
• Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
• Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Xu XR, Huang J, Xu ZG, et al. (2002). "Insight into hepatocellular carcinogenesis at transcriptome level by comparing gene expression profiles of hepatocellular carcinoma with those of corresponding noncancerous liver". Proc. Natl. Acad. Sci. U.S.A. 98 (26): 15089–94. doi:10.1073/pnas.241522398. PMC 64988. PMID 11752456.