SPAG9

Sperm associated antigen 9
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 2W83​
Identifiers
Symbols	SPAG9 ; CT89; HLC-6; HLC4; HLC6; JIP-4; JIP4; JLP; PHET; PIG6
External IDs	OMIM: 605430 MGI: 1918084 HomoloGene: 2954 GeneCards: SPAG9 Gene
Gene ontology Molecular function • MAP-kinase scaffold activity • protein binding • JUN kinase binding • kinesin binding • receptor signaling complex scaffold activity • mitogen-activated protein kinase p38 binding Cellular component • acrosomal vesicle • cytoplasm • cytosol • integral component of membrane • perinuclear region of cytoplasm • extracellular exosome Biological process • activation of JUN kinase activity • spermatogenesis • positive regulation of cell migration • retrograde transport, endosome to Golgi • muscle cell differentiation • positive regulation of neuron differentiation • striated muscle cell differentiation • positive regulation of muscle cell differentiation • protein homooligomerization • negative regulation of protein homodimerization activity Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	9043	70834
Ensembl	ENSG00000008294	ENSMUSG00000020859
UniProt	O60271	Q58A65
RefSeq (mRNA)	NM_001130527	NM_001025428
RefSeq (protein)	NP_001123999	NP_001020599
Location (UCSC)	Chr 17: 50.96 – 51.12 Mb	Chr 11: 94 – 94.13 Mb
PubMed search

C-jun-amino-terminal kinase-interacting protein 4 is an enzyme that in humans is encoded by the SPAG9 gene.^[1][2][3]

Function

Extracellular signals are transduced into cells through mitogen-activated protein kinases. The structural organization of these kinases into specific signaling domains is facilitated by scaffolding proteins involved in closely tethering different kinases so that successive phosphorylation events can occur. The protein encoded by this gene is a scaffolding protein that brings together mitogen-activated protein kinases and their transcription factor targets for the activation of specific signaling pathways. This gene which is abundantly expressed in testicular haploid germ cells encodes a protein that is recognized by sperm-agglutinating antibodies and implicated in infertility.^[3]

Interactions

SPAG9 has been shown to interact with MAX.^[4]

References

1. Shankar S, Mohapatra B, Suri A (Feb 1998). "Cloning of a novel human testis mRNA specifically expressed in testicular haploid germ cells, having unique palindromic sequences and encoding a leucine zipper dimerization motif". Biochemical and Biophysical Research Communications 243 (2): 561–5. doi:10.1006/bbrc.1997.7943. PMID 9480848.
2. Bowman AB, Kamal A, Ritchings BW, Philp AV, McGrail M, Gindhart JG, Goldstein LS (Nov 2000). "Kinesin-dependent axonal transport is mediated by the sunday driver (SYD) protein". Cell 103 (4): 583–94. doi:10.1016/S0092-8674(00)00162-8. PMID 11106729.
3. "Entrez Gene: SPAG9 sperm associated antigen 9".
4. Lee CM, Onésime D, Reddy CD, Dhanasekaran N, Reddy EP (Oct 2002). "JLP: A scaffolding protein that tethers JNK/p38MAPK signaling modules and transcription factors". Proceedings of the National Academy of Sciences of the United States of America 99 (22): 14189–94. doi:10.1073/pnas.232310199. PMC 137859. PMID 12391307.

Further reading

• Ichijo H (Nov 1999). "From receptors to stress-activated MAP kinases". Oncogene 18 (45): 6087–93. doi:10.1038/sj.onc.1203129. PMID 10557099.
• Nakajima D, Okazaki N, Yamakawa H, Kikuno R, Ohara O, Nagase T (Jun 2002). "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones". DNA Research 9 (3): 99–106. doi:10.1093/dnares/9.3.99. PMID 12168954.
• Nagase T, Ishikawa K, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (Feb 1998). "Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Research 5 (1): 31–9. doi:10.1093/dnares/5.1.31. PMID 9628581.
• Lee CM, Onésime D, Reddy CD, Dhanasekaran N, Reddy EP (Oct 2002). "JLP: A scaffolding protein that tethers JNK/p38MAPK signaling modules and transcription factors". Proceedings of the National Academy of Sciences of the United States of America 99 (22): 14189–94. doi:10.1073/pnas.232310199. PMC 137859. PMID 12391307.
• Yasuoka H, Ihn H, Medsger TA, Hirakata M, Kawakami Y, Ikeda Y, Kuwana M (Dec 2003). "A novel protein highly expressed in testis is overexpressed in systemic sclerosis fibroblasts and targeted by autoantibodies". Journal of Immunology 171 (12): 6883–90. doi:10.4049/jimmunol.171.12.6883. PMID 14662895.
• Bouwmeester T, Bauch A, Ruffner H, Angrand PO, Bergamini G, Croughton K, Cruciat C, Eberhard D, Gagneur J, Ghidelli S, Hopf C, Huhse B, Mangano R, Michon AM, Schirle M, Schlegl J, Schwab M, Stein MA, Bauer A, Casari G, Drewes G, Gavin AC, Jackson DB, Joberty G, Neubauer G, Rick J, Kuster B, Superti-Furga G (Feb 2004). "A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway". Nature Cell Biology 6 (2): 97–105. doi:10.1038/ncb1086. PMID 14743216.
• Brill LM, Salomon AR, Ficarro SB, Mukherji M, Stettler-Gill M, Peters EC (May 2004). "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry". Analytical Chemistry 76 (10): 2763–72. doi:10.1021/ac035352d. PMID 15144186.
• Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP (Aug 2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the United States of America 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
• Jagadish N, Rana R, Selvi R, Mishra D, Garg M, Yadav S, Herr JC, Okumura K, Hasegawa A, Koyama K, Suri A (Jul 2005). "Characterization of a novel human sperm-associated antigen 9 (SPAG9) having structural homology with c-Jun N-terminal kinase-interacting protein". The Biochemical Journal 389 (Pt 1): 73–82. doi:10.1042/BJ20041577. PMC 1184539. PMID 15693750.
• Jagadish N, Rana R, Mishra D, Kumar M, Suri A (Jun 2005). "Sperm associated antigen 9 (SPAG9): a new member of c-Jun NH2 -terminal kinase (JNK) interacting protein exclusively expressed in testis". The Keio Journal of Medicine 54 (2): 66–71. doi:10.2302/kjm.54.66. PMID 16077255.
• Guinn BA, Bland EA, Lodi U, Liggins AP, Tobal K, Petters S, Wells JW, Banham AH, Mufti GJ (Oct 2005). "Humoral detection of leukaemia-associated antigens in presentation acute myeloid leukaemia". Biochemical and Biophysical Research Communications 335 (4): 1293–304. doi:10.1016/j.bbrc.2005.08.024. PMID 16112646.
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
• Rana R, Jagadish N, Garg M, Mishra D, Dahiya N, Chaurasiya D, Suri A (Feb 2006). "Small interference RNA-mediated knockdown of sperm associated antigen 9 having structural homology with c-Jun N-terminal kinase-interacting protein". Biochemical and Biophysical Research Communications 340 (1): 158–64. doi:10.1016/j.bbrc.2005.11.168. PMID 16356479.
• Rana R, Jagadish N, Garg M, Mishra D, Dahiya N, Chaurasiya D, Suri A (Nov 2006). "Immunogenicity study of recombinant human sperm-associated antigen 9 in bonnet macaque (Macaca radiata)". Human Reproduction 21 (11): 2894–900. doi:10.1093/humrep/del068. PMID 16959808.