Saturated mutagenesis

Saturation mutagenesis is a form of random mutagenesis, in which one tries to generate all possible (or as close to as possible) mutations at a specific site, or narrow region of a gene. This is a common technique used in directed evolution.

Further reading

• Reetz, M. T.; Carballeira J. D. (2007). "Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes". Nature Prot. 2 (4): 891–903. doi:10.1038/nprot.2007.72. PMID 17446890.

• Chica, Robert A.; et al. (2005). "Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design". Current Opinion in Biotechnology 16 (4): 378–384. doi:10.1016/j.copbio.2005.06.004. PMID 15994074.