PPP1R9B

Protein phosphatase 1, regulatory subunit 9B
PDB rendering based on 2g5m.
Identifiers
Symbols	PPP1R9B ; PPP1R6; PPP1R9; SPINO; Spn
External IDs	OMIM: 603325 MGI: 2387581 HomoloGene: 32787 GeneCards: PPP1R9B Gene
Gene ontology Molecular function • actin binding • protein phosphatase inhibitor activity • protein binding • protein phosphatase 1 binding Cellular component • protein phosphatase type 1 complex • nucleoplasm • cytoplasm • cytoskeleton • plasma membrane • adherens junction • lamellipodium • filopodium • ruffle membrane • dendritic spine Biological process • regulation of cell growth by extracellular stimulus • cell cycle arrest • regulation of exit from mitosis • nervous system development • RNA splicing • cell migration • cell differentiation • negative regulation of cell growth • regulation of cell proliferation • negative regulation of catalytic activity • filopodium assembly • modulation of synaptic transmission • cellular response to morphine • regulation of opioid receptor signaling pathway Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	84687	217124
Ensembl	ENSG00000108819	ENSMUSG00000038976
UniProt	Q96SB3	Q6R891
RefSeq (mRNA)	NM_032595	NM_172261
RefSeq (protein)	NP_115984	NP_758465
Location (UCSC)	Chr 17: 50.13 – 50.15 Mb	Chr 11: 94.99 – 95.01 Mb
PubMed search

Neurabin-2 is a protein that in humans is encoded by the PPP1R9B gene.^[1][2]

Spinophilin is a regulatory subunit of protein phosphatase-1 catalytic subunit (PP1; see MIM 176875) and is highly enriched in dendritic spines, specialized protrusions from dendritic shafts that receive most of the excitatory input in the central nervous system (Allen et al., 1997).[supplied by OMIM]^[2]

Interactions

PPP1R9B has been shown to interact with PPP1CB,^[3] PPP1CA,^[3] Dopamine receptor D2,^[4] P16,^[5] PPP1CC,^[3][4] T-cell lymphoma invasion and metastasis-inducing protein 1^[6] and PPP1R2.^[7]

References

1. Allen PB, Ouimet CC, Greengard P (Oct 1997). "Spinophilin, a novel protein phosphatase 1 binding protein localized to dendritic spines". Proc Natl Acad Sci U S A 94 (18): 9956–61. doi:10.1073/pnas.94.18.9956. PMC 23308. PMID 9275233.
2. "Entrez Gene: PPP1R9B protein phosphatase 1, regulatory (inhibitor) subunit 9B".
3. Hsieh-Wilson, L C; Allen P B; Watanabe T; Nairn A C; Greengard P (Apr 1999). "Characterization of the neuronal targeting protein spinophilin and its interactions with protein phosphatase-1". Biochemistry (UNITED STATES) 38 (14): 4365–73. doi:10.1021/bi982900m. ISSN 0006-2960. PMID 10194355.
4. Smith, F D; Oxford G S; Milgram S L (Jul 1999). "Association of the D2 dopamine receptor third cytoplasmic loop with spinophilin, a protein phosphatase-1-interacting protein". J. Biol. Chem. (UNITED STATES) 274 (28): 19894–900. doi:10.1074/jbc.274.28.19894. ISSN 0021-9258. PMID 10391935.
5. Vivo, M; Calogero R A; Sansone F; Calabrò V; Parisi T; Borrelli L; Saviozzi S; La Mantia G (Apr 2001). "The human tumor suppressor arf interacts with spinophilin/neurabin II, a type 1 protein-phosphatase-binding protein". J. Biol. Chem. (United States) 276 (17): 14161–9. doi:10.1074/jbc.M006845200. ISSN 0021-9258. PMID 11278317.
6. Buchsbaum, Rachel J; Connolly Beth A; Feig Larry A (May 2003). "Regulation of p70 S6 kinase by complex formation between the Rac guanine nucleotide exchange factor (Rac-GEF) Tiam1 and the scaffold spinophilin". J. Biol. Chem. (United States) 278 (21): 18833–41. doi:10.1074/jbc.M207876200. ISSN 0021-9258. PMID 12531897.
7. Terry-Lorenzo, Ryan T; Elliot Elizabeth; Weiser Douglas C; Prickett Todd D; Brautigan David L; Shenolikar Shirish (Nov 2002). "Neurabins recruit protein phosphatase-1 and inhibitor-2 to the actin cytoskeleton". J. Biol. Chem. (United States) 277 (48): 46535–43. doi:10.1074/jbc.M206960200. ISSN 0021-9258. PMID 12270929.

Further reading

• Burnett PE, Blackshaw S, Lai MM, et al. (1998). "Neurabin is a synaptic protein linking p70 S6 kinase and the neuronal cytoskeleton". Proc. Natl. Acad. Sci. U.S.A. 95 (14): 8351–6. doi:10.1073/pnas.95.14.8351. PMC 20979. PMID 9653190.
• Hsieh-Wilson LC, Allen PB, Watanabe T, et al. (1999). "Characterization of the neuronal targeting protein spinophilin and its interactions with protein phosphatase-1". Biochemistry 38 (14): 4365–73. doi:10.1021/bi982900m. PMID 10194355.
• Smith FD, Oxford GS, Milgram SL (1999). "Association of the D2 dopamine receptor third cytoplasmic loop with spinophilin, a protein phosphatase-1-interacting protein". J. Biol. Chem. 274 (28): 19894–900. doi:10.1074/jbc.274.28.19894. PMID 10391935.
• Stephens DJ, Banting G (1999). "Direct interaction of the trans-Golgi network membrane protein, TGN38, with the F-actin binding protein, neurabin". J. Biol. Chem. 274 (42): 30080–6. doi:10.1074/jbc.274.42.30080. PMID 10514494.
• Richman JG, Brady AE, Wang Q, et al. (2001). "Agonist-regulated Interaction between alpha2-adrenergic receptors and spinophilin". J. Biol. Chem. 276 (18): 15003–8. doi:10.1074/jbc.M011679200. PMID 11154706.
• Vivo M, Calogero RA, Sansone F, et al. (2001). "The human tumor suppressor arf interacts with spinophilin/neurabin II, a type 1 protein-phosphatase-binding protein". J. Biol. Chem. 276 (17): 14161–9. doi:10.1074/jbc.M006845200. PMID 11278317.
• Grossman SD, Hsieh-Wilson LC, Allen PB, et al. (2003). "The actin-binding domain of spinophilin is necessary and sufficient for targeting to dendritic spines". Neuromolecular Med. 2 (1): 61–9. doi:10.1385/NMM:2:1:61. PMID 12230305.
• Terry-Lorenzo RT, Elliot E, Weiser DC, et al. (2003). "Neurabins recruit protein phosphatase-1 and inhibitor-2 to the actin cytoskeleton". J. Biol. Chem. 277 (48): 46535–43. doi:10.1074/jbc.M206960200. PMID 12270929.
• Hsieh-Wilson LC, Benfenati F, Snyder GL, et al. (2003). "Phosphorylation of spinophilin modulates its interaction with actin filaments". J. Biol. Chem. 278 (2): 1186–94. doi:10.1074/jbc.M205754200. PMID 12417592.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Buchsbaum RJ, Connolly BA, Feig LA (2003). "Regulation of p70 S6 kinase by complex formation between the Rac guanine nucleotide exchange factor (Rac-GEF) Tiam1 and the scaffold spinophilin". J. Biol. Chem. 278 (21): 18833–41. doi:10.1074/jbc.M207876200. PMID 12531897.
• Tsukada M, Prokscha A, Oldekamp J, Eichele G (2004). "Identification of neurabin II as a novel doublecortin interacting protein". Mech. Dev. 120 (9): 1033–43. doi:10.1016/S0925-4773(03)00177-1. PMID 14550532.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Law AJ, Weickert CS, Hyde TM, et al. (2004). "Reduced spinophilin but not microtubule-associated protein 2 expression in the hippocampal formation in schizophrenia and mood disorders: molecular evidence for a pathology of dendritic spines". The American Journal of Psychiatry 161 (10): 1848–55. doi:10.1176/appi.ajp.161.10.1848. PMID 15465982.