Starch analysis

Rice starch grains with angular outline and clumping attraction

Starch analysis or starch grain analysis is a technique that is useful in archaeological research to determine plant taxa. In certain samples of food plants, spices, drugs and desiccated archaeological plant matter, the histological elements can survive and thus be identified but in other samples, like carbonized (burnt) or older materials this is extremely difficult. However starch grains are much more hardy. The technique relies on the fact that a researcher can analyze or microscopically observe starch grains found on artifacts or in soils. Starch grains, ubiquitous in plants, have individual characteristics and resistance to grinding and drying and even to light burning so they are often preserved when other plant remains are lost - thus providing an additional tool to understand the past use of plants.

Usefulness

Starch grain analysis is not a perfect science, however, plant starch grain analysis is a diagnostic feature of multiple applications according to the peculiarities and to the origin of the plant material.[1] The size, shape and structure of grains from plant species, varies little, which can lead to identification. Starch grains have been removed and identified from stone tools, ceramic sherds, organic materials, dental calculus, and sediments[2] and animal remains to determine diet and when humans began to exploit wild food varieties.[3]

Disadvantages

In some cases the grains can become degraded. Factors such as heat and water absorption may affect the structure of the grains, making identification more difficult. Even if the remains are well preserved, water logging, dehydration, desiccation or damage from fungi can destroy the starch. In some cases, even within the same species, starch grains can differ in shape and size and the size of the grain affects its survivability in the archaeological record (Haslam 2004).

Biology of starch

Starch is produced in plants as a form of energy storage through the process of photosynthesis. When the plant is in need of energy, the stored starch is converted back into glucose.[4]

Starch grain identification

There are two basic methods for identifying starch:

See also: Microscopy
Starch, 800x magnified, under polarized light, showing characteristic extinction cross

Starch grains are typically microscopically identified with either optical or electron microscopy. Starch grains can become clearer if they are stained a darker color with Iodine Stains. Logol's Iodine is one, used for staining starch because iodine reagents easily bind to starch but less easily to other materials. Features that allow identification of starch grains include: presence of hilum (core of the grain), lamellae (or growth layers), birefringence, and extinction cross (a cross shape, visible on grains under revolving polarized light) which are visible with a microscope and shape and size.[2]

Low magnification

Archaeological research focused on residue adhering to artifacts start at lower magnifications, commonly using a stereoscope. Most data obtained at this stage is qualitative, an important first stage to fuller analysis. Magnifications of between x10 and x50 are sufficient to locate target residues, describe features and confirm internal structures of the identified residues.[5]

High magnification

Modern light, high powered microscopes have an internal light source, allowing illuminaton with both transmitted and reflected light. These microscopes can provide a magnification of up to x1000: good enough to provide clear images of starch granules as small as a few micrometres in diameter.[6]

Starch granules show different sizes. For example;

but all are generally under 100 micrometres in size, and are, therefore, best observed under compound microscopes equipped with various lighting conditions and magnifications from x200 to x800.[7]

The starch grains are also compared to standard reference collections for comparison. Archaeologists and researchers can consider four issues in classification of the plant(s) and its use(s):

Starch in sediments

Starch granules retrieved from sediments are used to reconstruct the habitats associated with human land use. Such studies address two areas of interest to the archaeologist:

The stages involved in the analysis of starch from sediments are; sampling, extraction of starch, slide mounting and viewing, and interpretation.[9]

Sampling

Sampling a sediment core or stratigraphic profile to gather information about an environment requires a detailed understanding of the way the sediments were formed.[10]

Extraction

Most extraction techniques follow a general methodology of:

Slide mounting and viewing

Starch granules are mounted onto a slide, using a variety of mounting medias including, but not limited, to water, glycerol, and glycerine jelly. It is important that the material is dried thoroughly before being mounted to ensure that no further degradation of the sample occurs. The slide is then viewed, as appropriate, for identification and counting.[12]

Interpretation

After the starch granules have been examined, the findings are then recorded and interpreted with respect to the research questions that are being investigated.

Starch on artifacts

Artifacts collect starch granules and protect them from decay due to microorganisms, thus providing excellent conditions for long-term preservation. The analysis may focus on the function of the tool, to examine a broader range of human behaviour but starch analysis also allows insights into craft activities involving the preparation of adhesives, medicines, or other nonfood items.[13]

Modified starch

Starch can also be investigated when it is not in its raw form. For example, Modified starch is created when the morphological or physico-chemical structure of native starch is disrupted in some way, such as in food preparation. The most common way to modify starch is to apply heat. Cooking pits, hearths, and ovens that may have come into contact with starchy material yield modified starches which can provide other insights.

Modified starch is only likely to be preserved under specific conditions, such as arid regions because of its susceptibility to organic decay. Studies of ancient modified starch aid understanding of ancient food technology, variations in cuisine among different social groups, as well as provide an understanding the function of ancient food-processing equipment.[14]

Preserved forms of modified starch include:

See also

Field, J. Cosgrove, R. Fullager, R. & Lance, B. (2011) "Starch residues on grinding stones in private collections: a study of morahs from the tropical rainforests of NE Queensland" in Haslam, M. Robertson, G. Crowther, A. Nugent, S. & Kirkwood,L.(Eds) Archaeological Science Under a Microscope (Terra Australis 30) Studies in Residue and Ancient DNA Analysis in Honour of Thomas H. Loy. ANU E Press. Canbarra. 228-238

Hather, J.G. (ed.) 1994. Tropical Archaeobotany: Applications and New Developments, pp. 86-114. Routledge, London.

Messner, Timothy C. 2011. Acorns and Bitter Roots: Starch Grain Research in the Prehistoric Eastern Woodlands. University of Alabama Press, Tuscaloosa, AL.

Torrence, Robin, and Huw Barton, editors. 2006. Ancient Starch Research. Left Coast Press, Walnut Creek, CA.

References

  1. Cortella, A. R.; Pochettino, M. L. (1994). "Starch grain analysis as a Microscopic Diagnostic Feature in the Identification of Plant Material". Economic Botany 48 (2): 171–181. doi:10.1007/BF02908212. ISSN 0013-0001.
  2. 1 2 Clarissa Cagnato. "Starch Grain Analysis - Paleoethnobotany". Retrieved 2014-06-25.
  3. Nature 430, 670-673 (5 August 2004) | doi:10.1038/nature02734; Received 4 May 2004; Accepted 4 June 2004
  4. Torrence 2006, p. 35,36
  5. Torrence 2006, p. 47,48
  6. Torrence 2006, p. 48,49
  7. Torrence 2006, p. 47
  8. Torrence 2006, pp. 115–143
  9. Torrence 2006, p. 145
  10. Torrence 2006, pp. 145–151
  11. Torrence 2006, pp. 151–161
  12. Torrence 2006, pp. 161–163
  13. Torrence 2006, pp. 185–189
  14. Torrence 2006, p. 205
  15. Torrence 2006, pp. 206–207

Torrence, Robin (2006), Ancient Starch Research, Walnut Creek, CA: Left Coast Press Inc., ISBN 1-59874-018-0 

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