| Rank | Heat | Lane | Name | Nationality | Time | Notes |
| 1 | 6 | 4 | Proud, BenjaminBenjamin Proud | England | 23.17 | Q |
| 2 | 5 | 6 | Schooling, JosephJoseph Schooling | Singapore | 23.43 | Q, NR |
| 3 | 5 | 4 | le Clos, ChadChad le Clos | South Africa | 23.65 | Q |
| 4 | 5 | 5 | Barrett, AdamAdam Barrett | England | 23.68 | Q |
| 5 | 6 | 6 | Dunford, JasonJason Dunford | Kenya | 23.76 | Q |
| 6 | 7 | 4 | Schoeman, RolandRoland Schoeman | South Africa | 23.85 | Q |
| 7 | 6 | 5 | Hadler, JaydenJayden Hadler | Australia | 23.87 | Q |
| 8 | 6 | 3 | Wright, ChrisChris Wright | Australia | 23.91 | Q |
| 9 | 7 | 2 | Fraser, BrettBrett Fraser | Cayman Islands | 24.01 | Q |
| 10 | 7 | 6 | Allen, ColemanColeman Allen | Canada | 24.25 | Q |
| 11 | 5 | 3 | Laxton, TomTom Laxton | Wales | 24.28 | Q |
| 12 | 7 | 5 | To, KennethKenneth To | Australia | 24.50 | Q |
| 13 | 6 | 2 | Assaad, GamalGamal Assaad | Canada | 24.66 | Q |
| 14 | 7 | 3 | Burrows, ElvisElvis Burrows | Bahamas | 24.83 | Q |
| 15 !=15 | 5 | 2 | Bakhtiarov, AlexandreAlexandre Bakhtiarov | Cyprus | 25.16 | Q |
| 7 | 8 | Munro, MilesMiles Munro | Guernsey | 25.16 | Q |
| 17 | 3 | 5 | Gallichan, TomTom Gallichan | Jersey | 25.34 | |
| 18 | 7 | 1 | Goveia, RalphRalph Goveia | Zambia | 25.35 | |
| 19 | 7 | 7 | Sau-Paea, IfalemiIfalemi Sau-Paea | Tonga | 25.40 | |
| 20 | 4 | 5 | Munn, ConorConor Munn | Northern Ireland | 25.57 | |
| 21 | 6 | 7 | Coulter, CurtisCurtis Coulter | Northern Ireland | 25.73 | |
| 22 !=22 | 5 | 1 | Vincent, BradleyBradley Vincent | Mauritius | 25.79 | |
| 6 | 8 | Wynter, TimothyTimothy Wynter | Jamaica | 25.79 | |
| 24 | 5 | 7 | de Silva, CheranthaCherantha de Silva | Sri Lanka | 25.86 | |
| 25 | 4 | 4 | Malani, MeliMeli Malani | Fiji | 25.90 | |
| 26 | 4 | 3 | Mohamed, IssaIssa Mohamed | Kenya | 26.00 | |
| 27 | 5 | 8 | Daniel, JoshuaJoshua Daniel | Saint Lucia | 26.04 | |
| 28 | 6 | 1 | Hollingsworth, ThomasThomas Hollingsworth | Guernsey | 26.22 | |
| 29 | 4 | 6 | Beaton, XanderXander Beaton | Guernsey | 26.53 | |
| 30 | 4 | 1 | Viktora, AdamAdam Viktora | Seychelles | 26.86 | |
| 31 | 3 | 7 | Kawale, StanfordStanford Kawale | Papua New Guinea | 26.93 | |
| 32 | 3 | 4 | Sanderson, JamesJames Sanderson | Gibraltar | 27.10 | |
| 33 | 2 | 5 | Jackson, AbeikuAbeiku Jackson | Ghana | 27.21 | |
| 34 | 3 | 1 | Hilal, Hilal HemedHilal Hemed Hilal | Tanzania | 27.28 | |
| 35 | 3 | 2 | Khan, SikandarSikandar Khan | Pakistan | 27.35 | |
| 36 | 3 | 3 | Cuna, EmidioEmidio Cuna | Mozambique | 27.44 | |
| 37 | 3 | 6 | Mweetwa, MilimoMilimo Mweetwa | Zambia | 27.70 | |
| 38 | 4 | 7 | Ibrahim, NishwanNishwan Ibrahim | Maldives | 27.79 | |
| 39 | 2 | 3 | Addo, KwakuKwaku Addo | Ghana | 27.80 | |
| 40 | 1 | 4 | Ghadiyali, AmmaarAmmaar Ghadiyali | Tanzania | 28.11 | |
| 41 | 3 | 8 | Regis, ChrisChris Regis | Grenada | 28.35 | |
| 42 | 2 | 4 | Akara, CollinCollin Akara | Papua New Guinea | 28.48 | |
| 43 | 2 | 7 | Kisulo, ArnoldArnold Kisulo | Uganda | 28.61 | |
| 44 | 2 | 6 | Ollivierre, CoreyCorey Ollivierre | Grenada | 28.72 | |
| 45 | 4 | 8 | Bandey, HarisHaris Bandey | Pakistan | 28.91 | |
| 46 | 2 | 2 | Sylvester, NikolasNikolas Sylvester | Saint Vincent and the Grenadines | 29.03 | |
| 47 | 4 | 2 | Adnan, MohamedMohamed Adnan | Maldives | 29.46 | |
| 48 | 1 | 5 | Gooding, DillonDillon Gooding | Saint Vincent and the Grenadines | 30.62 | |
| 49 | 1 | 3 | Halbich, StormStorm Halbich | Saint Vincent and the Grenadines | 31.09 | |
| 50 | 2 | 1 | Rukundo, PatrickPatrick Rukundo | Rwanda | 31.46 | |