Symmetry breaking and cortical rotation

This article is about the concept in biology. For the concept in physics, see Symmetry breaking.

Symmetry breaking is the process by which uniformity is broken, or the number of points to view invariance are reduced, to generate a more structured and improbable state.[1] That is to say, symmetry breaking is the event where symmetry along a particular axis is lost to establish a polarity. Polarity is a measure for a biological system to distinguish poles along an axis. This measure is important because it is the first step to building complexity. For example, during organismal development, one of the first steps for the embryo is to distinguish its dorsal-ventral axis. The symmetry-breaking event that occurs here will determine which end of this axis will be the ventral side, and which end will be the dorsal side. Once this distinction is made, then all the structures that are located along this axis can develop at the proper location. As an example, during human development, the embryo needs to establish where is ‘back’ and where is ‘front’ before complex structures, such as the spine and lungs, can develop in the right location (where the lungs is placed ‘in front’ of the spine). This relationship between symmetry breaking and complexity was articulated by P.W. Anderson. He speculated that increasing levels of broken symmetry in many-body systems correlates with increasing complexity and functional specialization.[2] In a biological perspective, the more complex an organism is, the higher number of symmetry-breaking events can be found. Without symmetry breaking, building complexity in organisms would be very difficult.

The importance of symmetry breaking in biology is also reflected in the fact that it’s found at all scales. Symmetry breaking can be found at the macromolecular level,[3] at the subcellular level[4] and even at the tissues and organ level.[5] It’s also interesting to note that most asymmetry on a higher scale is a reflection of symmetry breaking on a lower scale. Cells first need to establish a polarity through a symmetry-breaking event before tissues and organs themselves can be polar. For example, one model proposes that left-right body axis asymmetry in vertebrates is determined by asymmetry of cilia rotation during early development, which will produces a constant, unidirectional flow.[6][7] However there is also evidence that earlier asymmetries in serotonin distribution and ion-channel mRNA and protein localization occur in zebrafish, chicken and Xenopus development,[8][9][10] and similar to observations of intrinsic chirality generated by the cytoskeleton[11][12] leading to organ and whole organism asymmetries in Arabidopsis[13][14][15][16] this itself seems to be controlled from the macromolecular level by the cytoskeleton.[10]

There are several examples of symmetry breaking that are currently being studied. One of the most studied examples is the cortical rotation during Xenopus development, where this rotation acts as the symmetry-breaking event that determines the dorsal-ventral axis of the developing embryo. This example is discussed in more detail below.
Another example that involves symmetry breaking is the establishment of dendrites and axon during neuron development, and the PAR protein network in C. elegans. It is thought that a protein called shootin-1 determines which outgrowth in neurons eventually becomes the axon, at it does this by breaking symmetry and accumulating in only one outgrowth.[17] The PAR protein network works under similar mechanisms, where the certain PAR proteins, which are initially homogenous throughout the cell, break their symmetry and are segregated to different ends of the zygote to establish a polarity during development.[18]

Cortical Rotation

Cortical rotation is a phenomenon that seems to be limited to Xenopus and few ancient teleosts, however the underlying mechanisms of cortical rotation have conserved elements that are found in other chordates. Research in this area is on-going and changes to the model described below are to be expected. In fact, the origin of asymmetry in cell division, cell polarity and the mechanism that breaks the symmetry continue to be topics of intense research. Since the early 1990s, many discoveries have been made leading to a sound model of the mechanism for symmetry breaking. This article will focus solely on symmetry breaking in the Xenopus embryo, an animal model that has wide application.

A sperm can bind a Xenopus egg at any position of the pigmented animal hemisphere; however once bound this position then determines the dorsal side of the animal. The dorsal side of the egg is always directly opposite the sperm entry point. The reason being the sperm's centriole acts as an organizing center for the egg’s microtubules. While this observation has been known for quite some time, the question of how all of this works is more complicated. The molecular mechanisms driving dorsal-ventral asymmetry are a fine example of simplicity and complexity inherent in biology.

Molecular mechanisms

A series of experiments utilizing UV irradiation, cold temperature and pressure (all of which cause microtubule depolymerization) demonstrated that without polymerized microtubules cortical rotation did not occur and resulted in a mutant ventral phenotype.[19] Another study also revealed that mutant phenotype could be rescued (returned to normal) by physically turning the embryo, thus mimicking cortical rotation and demonstrating that microtubules were not the determinant of dorsal development.[20] From this it was hypothesized that there were other elements within the embryo being moved during cortical rotation.

To identify these elements, researchers looked for mRNA and protein that demonstrated localization to either the vegetal pole or the dorsal side of the embryo to find candidates. The early candidates for the determinant were β-catenin and disheveled (Dsh).[21][22] When maternal β-catenin mRNA was degraded in the oocyte, the resulting embryo developed into mutant ventral phenotype and this could be rescued by injecting the fertilized egg with β-catenin mRNA. β-catenin is obversed to be enriched in the dorsal side of the embryo following cortical rotation. The Dsh protein was fused to a GFP and tracked during cortical rotation, it was observed to be in vesicles that were couriered along microtubules to the dorsal side. This led researchers to look into other candidates of the Wnt pathway. Wnt 11 was found to be located specifically at the vegetal pole prior to cortical rotation and is moved to the dorsal side where it activates the wnt signaling pathway.[23] VegT, a T-box transcription factor, is localized to the vegetal cortex and upon cortical rotation is released in a gradient fashion into the embryo to regulate mesoderm development.[24] VegT activates Wnt expression, so while not acted on or moved during cortical rotation, it is active in dorsal-ventral axis formation.

The question still remains, how are these molecules being moved to the dorsal side? This is still not completely known, however evidence suggests that microtubule bundles within the cortex are interacting with kinesin (plus-end directed) motors to become organized into parallel arrays within the cortex and this motion of the motors is the cause of the rotation of the cortex.[25] Also unclear is whether Wnt 11 is the main dorsal determinant or is β-catenin also required, as these two molecules have both been demonstrated to be necessary and sufficient for dorsal development. This along with all of the other factors are important for activating Nodal genes that propagate normal dorsoventral development.

For reviews of the general topic see.[26][27]

References

  1. ↑ Li, Rong; Bruce Bowerman (2010). "Symmetry Breaking in Biology". Cold Spring Harbor Perspectives in Biology 2 (3): a003475. doi:10.1101/cshperspect.a003475. PMC 2829966. PMID 20300216.
  2. ↑ Anderson, Philip W. (1972). "More is Different". Science 177 (4047): 393–396. doi:10.1126/science.177.4047.393. PMID 17796623.
  3. ↑ Wong, Fei (2009). "The Signaling Mechanisms Underlying Cell Polarity and Chemotaxis". Cold Spring Harbor Perspectives in Biology 1 (4): a002980. doi:10.1101/cshperspect.a002980. PMID 20066099.
  4. ↑ Dworkin, Jonathan (2009). "Cellular Polarity in Prokaryotic Organisms". Cold Spring Harbor Perspectives in Biology 1 (6): a003368. doi:10.1101/cshperspect.a003368. PMID 20457568.
  5. ↑ Nelson, James W. (2009). "Remodeling epithelial cell organization: Transitions between front-rear and apical-basal polarity". Cold Spring Harbor Perspectives in Biology 1 (1): a000513. doi:10.1101/cshperspect.a000513.
  6. ↑ Babu, Deepak; Sudipto Roy (2013). "Left–right asymmetry: cilia stir up new surprises in the node". Open Biology 3 (5).
  7. ↑ Kuznetsov, A. V.; Blinov, D. G.; Avramenko, A. A.; Shevchuk, I. V.; Tyrinov, A. I.; Kuznetsov, I. A. (13 December 2013). "Approximate modelling of the leftward flow and morphogen transport in the embryonic node by specifying vorticity at the ciliated surface". Journal of Fluid Mechanics 738: 492–521. doi:10.1017/jfm.2013.588.
  8. ↑ Fukumoto, Takahiro; Kema, Ido P.; Levin, Michael (2005-10-05). "Serotonin Signaling Is a Very Early Step in Patterning of the Left-Right Axis in Chick and Frog Embryos". Current Biology 15 (9): 794–803. doi:10.1016/j.cub.2005.03.044. ISSN 0960-9822. PMID 15886096.
  9. ↑ Aw, Sherry; Adams, Dany S.; Qiu, Dayong; Levin, Michael (2008-03-01). "H,K-ATPase protein localization and Kir4.1 function reveal concordance of three axes during early determination of left–right asymmetry". Mechanisms of Development 125 (3–4): 353–372. doi:10.1016/j.mod.2007.10.011. PMC 2346612. PMID 18160269.
  10. 1 2 Lobikin, Maria; Wang, Gang; Xu, Jingsong; Hsieh, Yi-Wen; Chuang, Chiou-Fen; Lemire, Joan M.; Levin, Michael (2012-07-31). "Early, nonciliary role for microtubule proteins in left–right patterning is conserved across kingdoms". Proceedings of the National Academy of Sciences 109 (31): 12586–12591. doi:10.1073/pnas.1202659109. ISSN 0027-8424. PMC 3412009. PMID 22802643.
  11. ↑ Xu, Jingsong; Keymeulen, Alexandra Van; Wakida, Nicole M.; Carlton, Pete; Berns, Michael W.; Bourne, Henry R. (2007-05-29). "Polarity reveals intrinsic cell chirality". Proceedings of the National Academy of Sciences 104 (22): 9296–9300. doi:10.1073/pnas.0703153104. ISSN 0027-8424. PMC 1890488. PMID 17517645.
  12. ↑ Wan, Leo Q.; Ronaldson, Kacey; Park, Miri; Taylor, Grace; Zhang, Yue; Gimble, Jeffrey M.; Vunjak-Novakovic, Gordana (2011-07-26). "Micropatterned mammalian cells exhibit phenotype-specific left-right asymmetry". Proceedings of the National Academy of Sciences 108 (30): 12295–12300. doi:10.1073/pnas.1103834108. ISSN 0027-8424. PMC 3145729. PMID 21709270.
  13. ↑ Nakamura, Masayoshi; Hashimoto, Takashi (2009-07-01). "A mutation in the Arabidopsis γ-tubulin-containing complex causes helical growth and abnormal microtubule branching". Journal of Cell Science 122 (13): 2208–2217. doi:10.1242/jcs.044131. ISSN 0021-9533. PMID 19509058.
  14. ↑ Abe, Tatsuya; Thitamadee, Siripong; Hashimoto, Takashi (2004-02-15). "Microtubule Defects and Cell Morphogenesis in the lefty1lefty2 Tubulin Mutant of Arabidopsis thaliana". Plant and Cell Physiology 45 (2): 211–220. doi:10.1093/pcp/pch026. ISSN 0032-0781. PMID 14988491.
  15. ↑ Ishida, Takashi; Hashimoto, Takashi (2007-07-20). "An Arabidopsis thaliana tubulin mutant with conditional root-skewing phenotype". Journal of Plant Research 120 (5): 635–640. doi:10.1007/s10265-007-0105-0. ISSN 0918-9440.
  16. ↑ Ishida, Takashi; Kaneko, Yayoi; Iwano, Megumi; Hashimoto, Takashi (2007-05-15). "Helical microtubule arrays in a collection of twisting tubulin mutants of Arabidopsis thaliana". Proceedings of the National Academy of Sciences 104 (20): 8544–8549. doi:10.1073/pnas.0701224104. ISSN 0027-8424. PMC 1895986. PMID 17488810.
  17. ↑ Toriyama, Michinori; Tadayuki Shimada; Ki Bum Kim; Mari Mitsuba; Eiko Nomura; Kazuhiro Katsuta; Yuichi Sakumura; Peter Roepstorff; Naoyuki Inagaki (2006). "Shootin1: A protein involved in the organization of an asymmetric signal for neuronal polarization". The Journal of Cell Biology 175 (1): 147–157. doi:10.1083/jcb.200604160.
  18. ↑ Motegi, Fumio; Geraldine Seydoux (2013). "The PAR network: redundancy and robustness in a symmetry-breaking system". Philosophical Transactions of the Royal Society 368 (1629): 20130010. doi:10.1098/rstb.2013.0010.
  19. ↑ Gerhart J, Danilchik M, Doniach T, Roberts S, Rowning B, Stewart R (1989). "Cortical rotation of the Xenopus egg: consequences for the anteroposterior pattern of embryonic dorsal development". Development 107 (Suppl): 37–51. PMID 2699856.
  20. ↑ Scharf SR, Gerhart JC (September 1980). "Determination of the dorsal-ventral axis in eggs of Xenopus laevis: complete rescue of UV-impaired eggs by oblique orientation before first cleavage". Dev. Biol. 79 (1): 181–98. doi:10.1016/0012-1606(80)90082-2. PMID 7409319.
  21. ↑ Heasman J, Crawford A, Goldstone K, Garner-Hamrick P, Gumbiner B, McCrea P, Kintner C, Noro CY, Wylie C (1994). "Overexpression of cadherins and underexpression of beta-catenin inhibit dorsal mesoderm induction in early Xenopus embryos". Cell 79 (5): 791–803. doi:10.1016/0092-8674(94)90069-8. PMID 7528101.
  22. ↑ Miller JR, Rowning BA, Larabell CA, Yang-Snyder JA, Bates RL, Moon RT (July 1999). "Establishment of the dorsal-ventral axis in Xenopus embryos coincides with the dorsal enrichment of dishevelled that is dependent on cortical rotation". J. Cell Biol. 146 (2): 427–37. doi:10.1083/jcb.146.2.427. PMC 2156185. PMID 10427095.
  23. ↑ Tao Q, Yokota C, Puck H, Kofron M, Birsoy B, Yan D, Asashima M, Wylie CC, Lin X, Heasman J (2005). "Maternal wnt11 activates the canonical wnt signaling pathway required for axis formation in Xenopus embryos". Cell 120 (6): 857–71. doi:10.1016/j.cell.2005.01.013. PMID 15797385.
  24. ↑ Zhang J, King ML (December 1996). "Xenopus VegT RNA is localized to the vegetal cortex during oogenesis and encodes a novel T-box transcription factor involved in mesodermal patterning". Development 122 (12): 4119–29. PMID 9012531.
  25. ↑ Marrari Y, Rouviere C, Houliston E (2004). "Complementary roles for dynein and kinesins in the Xenopus egg cortical rotation". Dev Biol 271 (1): 38–48. doi:10.1016/j.ydbio.2004.03.018. PMID 15196948.
  26. ↑ Weaver C, Kimelman D (2004). "Move it or lose it: axis specification in Xenopus". Development 131 (15): 3491–9. doi:10.1242/dev.01284. PMID 15262887.
  27. ↑ Schiffmann Y (2006). "Symmetry breaking and convergent extension in early chordate development". Prog Biophys Mol Biol 92 (2): 209–31. doi:10.1016/j.pbiomolbio.2005.10.002. PMID 16321426.
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