TAF7

TAF7 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 55kDa
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 4RGW​
Identifiers
Symbols	TAF7 ; TAF2F; TAFII55
External IDs	OMIM: 600573 MGI: 1346348 HomoloGene: 133942 GeneCards: TAF7 Gene
Gene ontology Molecular function • transcription factor activity, sequence-specific DNA binding • transcription coactivator activity • protein binding • transcription factor binding • histone acetyltransferase binding • vitamin D receptor binding • transcription regulatory region DNA binding • thyroid hormone receptor binding Cellular component • nucleoplasm • transcription factor TFIID complex • Golgi apparatus • positive transcription elongation factor complex b • transcription factor TFTC complex • MLL1 complex Biological process • negative regulation of transcription from RNA polymerase II promoter • spermine transport • DNA-templated transcription, initiation • transcription from RNA polymerase II promoter • transcription initiation from RNA polymerase II promoter • negative regulation of protein kinase activity • intracellular estrogen receptor signaling pathway • negative regulation of histone acetylation • negative regulation of transcription, DNA-templated • positive regulation of transcription from RNA polymerase II promoter • RNA polymerase II transcriptional preinitiation complex assembly Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	6879	24074
Ensembl	ENSG00000178913	ENSMUSG00000051316
UniProt	Q15545	Q9R1C0
RefSeq (mRNA)	NM_005642	NM_175770
RefSeq (protein)	NP_005633	NP_786964
Location (UCSC)	Chr 5: 141.26 – 141.32 Mb	Chr 18: 37.64 – 37.64 Mb
PubMed search

Transcription initiation factor TFIID subunit 7 also known as TAFII55 is a protein that in humans is encoded by the TAF7 gene.^[1]

Function

The intronless gene for this transcription coactivator is located between the protocadherin beta and gamma gene clusters on chromosome 5. The protein encoded by this gene is a component of the TFIID protein complex, a complex which binds to the TATA box in class II promoters and recruits RNA polymerase II and other factors. This particular subunit interacts with the largest TFIID subunit, as well as multiple transcription activators. The protein is required for transcription by promoters targeted by RNA polymerase II.^[2]

TAFII55_N
Identifiers
Symbol	TAFII55_N
Pfam	PF04658
InterPro	IPR006751
Available protein structures: Pfam structures PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

The general transcription factor, TFIID, consists of the TATA-binding protein (TBP) associated with a series of TBP-associated factors (TAFs) that together participate in the assembly of the transcription preinitiation complex. TAFII55 binds to TAFII250 and inhibits its acetyltransferase activity. The exact role of TAFII55 is currently unknown but studies have shown that it interacts with the C-jun pathway.^[3] The conserved region is situated towards the N-terminal of the protein.^[4] This entry talks about the N-terminal domain.

Interactions

TAF7 has been shown to interact with:

• TAF15,^[5]
• TAF1,^[4] and
• TATA binding protein.^[6][7]

References

1. Chiang CM, Roeder RG (February 1995). "Cloning of an intrinsic human TFIID subunit that interacts with multiple transcriptional activators". Science 267 (5197): 531–6. doi:10.1126/science.7824954. PMID 7824954.
2. "Entrez Gene: TAF7 TAF7 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 55kDa".
3. Munz C, Psichari E, Mandilis D, Lavigne AC, Spiliotaki M, Oehler T, Davidson I, Tora L, Angel P, Pintzas A (2003). "TAF7 (TAFII55) plays a role in the transcription activation by c-Jun". J. Biol. Chem. 278 (24): 21510–6. doi:10.1074/jbc.M212764200. PMID 12676957.
4. Gegonne A, Weissman JD, Singer DS (October 2001). "TAFII55 binding to TAFII250 inhibits its acetyltransferase activity". Proc. Natl. Acad. Sci. U.S.A. 98 (22): 12432–7. doi:10.1073/pnas.211444798. PMC 60071. PMID 11592977.
5. Bertolotti A, Melot T, Acker J, Vigneron M, Delattre O, Tora L (March 1998). "EWS, but not EWS-FLI-1, is associated with both TFIID and RNA polymerase II: interactions between two members of the TET family, EWS and hTAFII68, and subunits of TFIID and RNA polymerase II complexes". Mol. Cell. Biol. 18 (3): 1489–97. PMC 108863. PMID 9488465.
6. Pointud JC, Mengus G, Brancorsini S, Monaco L, Parvinen M, Sassone-Corsi P, Davidson I (May 2003). "The intracellular localisation of TAF7L, a paralogue of transcription factor TFIID subunit TAF7, is developmentally regulated during male germ-cell differentiation". J. Cell. Sci. 116 (Pt 9): 1847–58. doi:10.1242/jcs.00391. PMID 12665565.
7. Bellorini M, Lee DK, Dantonel JC, Zemzoumi K, Roeder RG, Tora L, Mantovani R (June 1997). "CCAAT binding NF-Y-TBP interactions: NF-YB and NF-YC require short domains adjacent to their histone fold motifs for association with TBP basic residues". Nucleic Acids Res. 25 (11): 2174–81. doi:10.1093/nar/25.11.2174. PMC 146709. PMID 9153318.

Further reading

• Zhou Q, Sharp PA (1995). "Novel mechanism and factor for regulation by HIV-1 Tat". EMBO J. 14 (2): 321–8. PMC 398086. PMID 7835343.
• Parada CA, Yoon JB, Roeder RG (1995). "A novel LBP-1-mediated restriction of HIV-1 transcription at the level of elongation in vitro". J. Biol. Chem. 270 (5): 2274–83. doi:10.1074/jbc.270.5.2274. PMID 7836461.
• Ou SH, Garcia-Martínez LF, Paulssen EJ, Gaynor RB (1994). "Role of flanking E box motifs in human immunodeficiency virus type 1 TATA element function". J. Virol. 68 (11): 7188–99. PMC 237158. PMID 7933101.
• Cross SH, Charlton JA, Nan X, Bird AP (1994). "Purification of CpG islands using a methylated DNA binding column". Nat. Genet. 6 (3): 236–44. doi:10.1038/ng0394-236. PMID 8012384.
• Kashanchi F, Piras G, Radonovich MF, Duvall JF, Fattaey A, Chiang CM, Roeder RG, Brady JN (1994). "Direct interaction of human TFIID with the HIV-1 transactivator tat". Nature 367 (6460): 295–9. doi:10.1038/367295a0. PMID 8121496.
• Wang Z, Morris GF, Rice AP, Xiong W, Morris CB (1996). "Wild-type and transactivation-defective mutants of human immunodeficiency virus type 1 Tat protein bind human TATA-binding protein in vitro". J. Acquir. Immune Defic. Syndr. Hum. Retrovirol. 12 (2): 128–38. doi:10.1097/00042560-199606010-00005. PMID 8680883.
• Lavigne AC, Mengus G, May M, Dubrovskaya V, Tora L, Chambon P, Davidson I (1996). "Multiple interactions between hTAFII55 and other TFIID subunits. Requirements for the formation of stable ternary complexes between hTAFII55 and the TATA-binding protein". J. Biol. Chem. 271 (33): 19774–80. doi:10.1074/jbc.271.33.19774. PMID 8702684.
• Pendergrast PS, Morrison D, Tansey WP, Hernandez N (1996). "Mutations in the carboxy-terminal domain of TBP affect the synthesis of human immunodeficiency virus type 1 full-length and short transcripts similarly". J. Virol. 70 (8): 5025–34. PMC 190456. PMID 8764009.
• Kashanchi F, Khleif SN, Duvall JF, Sadaie MR, Radonovich MF, Cho M, Martin MA, Chen SY, Weinmann R, Brady JN (1996). "Interaction of human immunodeficiency virus type 1 Tat with a unique site of TFIID inhibits negative cofactor Dr1 and stabilizes the TFIID-TFIIA complex". J. Virol. 70 (8): 5503–10. PMC 190508. PMID 8764062.
• Rowlands JC, McEwan IJ, Gustafsson JA (1996). "Trans-activation by the human aryl hydrocarbon receptor and aryl hydrocarbon receptor nuclear translocator proteins: direct interactions with basal transcription factors". Mol. Pharmacol. 50 (3): 538–48. PMID 8794892.
• Zhou Q, Sharp PA (1996). "Tat-SF1: cofactor for stimulation of transcriptional elongation by HIV-1 Tat". Science 274 (5287): 605–10. doi:10.1126/science.274.5287.605. PMID 8849451.
• García-Martínez LF, Ivanov D, Gaynor RB (1997). "Association of Tat with purified HIV-1 and HIV-2 transcription preinitiation complexes". J. Biol. Chem. 272 (11): 6951–8. doi:10.1074/jbc.272.11.6951. PMID 9054383.
• Dantonel JC, Murthy KG, Manley JL, Tora L (1997). "Transcription factor TFIID recruits factor CPSF for formation of 3' end of mRNA". Nature 389 (6649): 399–402. doi:10.1038/38763. PMID 9311784.
• Bertolotti A, Melot T, Acker J, Vigneron M, Delattre O, Tora L (1998). "EWS, but not EWS-FLI-1, is associated with both TFIID and RNA polymerase II: interactions between two members of the TET family, EWS and hTAFII68, and subunits of TFIID and RNA polymerase II complexes". Mol. Cell. Biol. 18 (3): 1489–97. PMC 108863. PMID 9488465.
• Fraser RA, Heard DJ, Adam S, Lavigne AC, Le Douarin B, Tora L, Losson R, Rochette-Egly C, Chambon P (1998). "The putative cofactor TIF1alpha is a protein kinase that is hyperphosphorylated upon interaction with liganded nuclear receptors". J. Biol. Chem. 273 (26): 16199–204. doi:10.1074/jbc.273.26.16199. PMID 9632676.
• Furukawa T, Tanese N (2000). "Assembly of partial TFIID complexes in mammalian cells reveals distinct activities associated with individual TATA box-binding protein-associated factors". J. Biol. Chem. 275 (38): 29847–56. doi:10.1074/jbc.M002989200. PMID 10896937.
• Yuan CX, Gurley WB (2000). "Potential targets for HSF1 within the preinitiation complex". Cell Stress Chaperones 5 (3): 229–42. doi:10.1379/1466-1268(2000)005<0229:PTFHWT>2.0.CO;2. PMC 312889. PMID 11005381.
• Wu Q, Zhang T, Cheng JF, Kim Y, Grimwood J, Schmutz J, Dickson M, Noonan JP, Zhang MQ, Myers RM, Maniatis T (2001). "Comparative DNA sequence analysis of mouse and human protocadherin gene clusters". Genome Res. 11 (3): 389–404. doi:10.1101/gr.167301. PMC 311048. PMID 11230163.
• Zhou T, Chiang CM (2001). "The intronless and TATA-less human TAF(II)55 gene contains a functional initiator and a downstream promoter element". J. Biol. Chem. 276 (27): 25503–11. doi:10.1074/jbc.M102875200. PMID 11340078.

External links

• FactorBook TAF7