TFAM

Transcription factor A, mitochondrial
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 3FGH​, 3TMM​, 3TQ6​, 4NNU​, 4NOD​
Identifiers
Symbols	TFAM ; MTTF1; MTTFA; TCF6; TCF6L1; TCF6L2; TCF6L3
External IDs	OMIM: 600438 MGI: 107810 HomoloGene: 31139 GeneCards: TFAM Gene
Gene ontology Molecular function • RNA polymerase II core promoter proximal region sequence-specific DNA binding • transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding • chromatin binding • transcription factor activity, sequence-specific DNA binding • protein binding • DNA binding, bending • heat shock protein binding • poly(A) RNA binding • mitochondrial light strand promoter sense binding Cellular component • nucleus • mitochondrion • mitochondrial matrix • cytosol • mitochondrial nucleoid Biological process • DNA-dependent DNA replication • chromatin remodeling • regulation of transcription from RNA polymerase I promoter • transcription from RNA polymerase II promoter • transcription from mitochondrial promoter • transcription initiation from mitochondrial promoter • organelle organization • mitochondrion organization • gene expression • mitochondrial respiratory chain complex assembly • positive regulation of transcription, DNA-templated • positive regulation of transcription from RNA polymerase II promoter Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	7019	21780
Ensembl	ENSG00000108064	ENSMUSG00000003923
UniProt	Q00059	P40630
RefSeq (mRNA)	NM_001270782	NM_009360
RefSeq (protein)	NP_001257711	NP_033386
Location (UCSC)	Chr 10: 58.39 – 58.4 Mb	Chr 10: 71.23 – 71.24 Mb
PubMed search

Mitochondrial transcription factor A, abbreviated as TFAM or mtTFA, is a protein that in humans is encoded by the TFAM gene.^[1][2]

Function

This gene encodes a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. TFAM bends mitochondrial promoter DNA to aid transcription of the mitochondrial genome. Studies in mice have demonstrated that this gene product is required to regulate the mitochondrial genome copy number and is essential for embryonic development. A mouse model for Kearns-Sayre syndrome was produced when expression of this gene was eliminated by targeted disruption in heart and muscle cells.^[2]

Interactions

TFAM has been shown to interact with TFB1M.^[3]

References

1. Tiranti V, Rossi E, Ruiz-Carrillo A, Rossi G, Rocchi M, DiDonato S, Zuffardi O, Zeviani M (Jul 1995). "Chromosomal localization of mitochondrial transcription factor A (TCF6), single-stranded DNA-binding protein (SSBP), and endonuclease G (ENDOG), three human housekeeping genes involved in mitochondrial biogenesis". Genomics 25 (2): 559–64. doi:10.1016/0888-7543(95)80058-T. PMID 7789991.
2. "Entrez Gene: TFAM transcription factor A, mitochondrial".
3. McCulloch V, Shadel GS (Aug 2003). "Human mitochondrial transcription factor B1 interacts with the C-terminal activation region of h-mtTFA and stimulates transcription independently of its RNA methyltransferase activity". Mol. Cell. Biol. 23 (16): 5816–24. doi:10.1128/MCB.23.16.5816-5824.2003. PMC 166325. PMID 12897151.

Further reading

• Tominaga K, Akiyama S, Kagawa Y, Ohta S (1992). "Upstream region of a genomic gene for human mitochondrial transcription factor 1.". Biochim. Biophys. Acta 1131 (2): 217–9. doi:10.1016/0167-4781(92)90082-b. PMID 1610904.
• Fisher RP, Lisowsky T, Parisi MA, Clayton DA (1992). "DNA wrapping and bending by a mitochondrial high mobility group-like transcriptional activator protein.". J. Biol. Chem. 267 (5): 3358–67. PMID 1737790.
• Parisi MA, Clayton DA (1991). "Similarity of human mitochondrial transcription factor 1 to high mobility group proteins.". Science 252 (5008): 965–9. doi:10.1126/science.2035027. PMID 2035027.
• Tominaga K, Hayashi J, Kagawa Y, Ohta S (1993). "Smaller isoform of human mitochondrial transcription factor 1: its wide distribution and production by alternative splicing.". Biochem. Biophys. Res. Commun. 194 (1): 544–51. doi:10.1006/bbrc.1993.1854. PMID 8333869.
• Larsson NG, Oldfors A, Garman JD, Barsh GS, Clayton DA (1997). "Down-regulation of mitochondrial transcription factor A during spermatogenesis in humans.". Hum. Mol. Genet. 6 (2): 185–91. doi:10.1093/hmg/6.2.185. PMID 9063738.
• Lezza AM, Pesce V, Cormio A, Fracasso F, Vecchiet J, Felzani G, Cantatore P, Gadaleta MN (2001). "Increased expression of mitochondrial transcription factor A and nuclear respiratory factor-1 in skeletal muscle from aged human subjects.". FEBS Lett. 501 (1): 74–8. doi:10.1016/S0014-5793(01)02628-X. PMID 11457459.
• Takamatsu C, Umeda S, Ohsato T, Ohno T, Abe Y, Fukuoh A, Shinagawa H, Hamasaki N, Kang D (2002). "Regulation of mitochondrial D-loops by transcription factor A and single-stranded DNA-binding protein.". EMBO Rep. 3 (5): 451–6. doi:10.1093/embo-reports/kvf099. PMC 1084112. PMID 11964388.
• Falkenberg M, Gaspari M, Rantanen A, Trifunovic A, Larsson NG, Gustafsson CM (2002). "Mitochondrial transcription factors B1 and B2 activate transcription of human mtDNA.". Nat. Genet. 31 (3): 289–94. doi:10.1038/ng909. PMID 12068295.
• Reyes A, Mezzina M, Gadaleta G (2002). "Human mitochondrial transcription factor A (mtTFA): gene structure and characterization of related pseudogenes.". Gene 291 (1-2): 223–32. doi:10.1016/S0378-1119(02)00600-5. PMID 12095695.
• Yoshida Y, Izumi H, Ise T, Uramoto H, Torigoe T, Ishiguchi H, Murakami T, Tanabe M, Nakayama Y, Itoh H, Kasai H, Kohno K (2002). "Human mitochondrial transcription factor A binds preferentially to oxidatively damaged DNA.". Biochem. Biophys. Res. Commun. 295 (4): 945–51. doi:10.1016/S0006-291X(02)00757-X. PMID 12127986.
• Garrido N, Griparic L, Jokitalo E, Wartiovaara J, van der Bliek AM, Spelbrink JN (2003). "Composition and dynamics of human mitochondrial nucleoids.". Mol. Biol. Cell 14 (4): 1583–96. doi:10.1091/mbc.E02-07-0399. PMC 153124. PMID 12686611.
• Yoshida Y, Izumi H, Torigoe T, Ishiguchi H, Itoh H, Kang D, Kohno K (2003). "P53 physically interacts with mitochondrial transcription factor A and differentially regulates binding to damaged DNA.". Cancer Res. 63 (13): 3729–34. PMID 12839966.
• McCulloch V, Shadel GS (2003). "Human mitochondrial transcription factor B1 interacts with the C-terminal activation region of h-mtTFA and stimulates transcription independently of its RNA methyltransferase activity.". Mol. Cell. Biol. 23 (16): 5816–24. doi:10.1128/MCB.23.16.5816-5824.2003. PMC 166325. PMID 12897151.
• Deschauer M, Kiefer R, Blakely EL, He L, Zierz S, Turnbull DM, Taylor RW (2003). "A novel Twinkle gene mutation in autosomal dominant progressive external ophthalmoplegia.". Neuromuscul. Disord. 13 (7-8): 568–72. doi:10.1016/S0960-8966(03)00071-3. PMID 12921794.
• Günther C, von Hadeln K, Müller-Thomsen T, Alberici A, Binetti G, Hock C, Nitsch RM, Stoppe G, Reiss J, Gal A, Finckh U (2005). "Possible association of mitochondrial transcription factor A (TFAM) genotype with sporadic Alzheimer disease.". Neurosci. Lett. 369 (3): 219–23. doi:10.1016/j.neulet.2004.07.070. PMID 15464268.
• Kanki T, Ohgaki K, Gaspari M, Gustafsson CM, Fukuoh A, Sasaki N, Hamasaki N, Kang D (2004). "Architectural role of mitochondrial transcription factor A in maintenance of human mitochondrial DNA.". Mol. Cell. Biol. 24 (22): 9823–34. doi:10.1128/MCB.24.22.9823-9834.2004. PMC 525493. PMID 15509786.
• Cam H, Balciunaite E, Blais A, Spektor A, Scarpulla RC, Young R, Kluger Y, Dynlacht BD (2004). "A common set of gene regulatory networks links metabolism and growth inhibition.". Mol. Cell 16 (3): 399–411. doi:10.1016/j.molcel.2004.09.037. PMID 15525513.