TMEM66

Store-operated calcium entry-associated regulatory factor
Identifiers
Symbols	SARAF ; FOAP-7; HSPC035; TMEM66; XTP3
External IDs	OMIM: 614768 HomoloGene: 9398 GeneCards: SARAF Gene
Gene ontology Molecular function • protein binding Cellular component • integral component of endoplasmic reticulum membrane Biological process • calcium ion transport • regulation of store-operated calcium entry Sources: Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	51669	67887
Ensembl	ENSG00000133872	ENSMUSG00000031532
UniProt	Q96BY9	Q8R3Q0
RefSeq (mRNA)	NM_001284239	NM_026432
RefSeq (protein)	NP_001271168	NP_080708
Location (UCSC)	Chr 8: 30.06 – 30.08 Mb	Chr 8: 34.15 – 34.17 Mb
PubMed search

SARAF is a protein that in humans is encoded by the SARAF gene, formerly known as TMEM66 (transmembrane protein 66).^[1][2]

Function

SARAF (TMEM66) is a negative regulator of the store-operated calcium channel (SOCE) into cells. SARAF is an endoplasmic reticulum (ER) membrane resident protein that associates with STIM1, to facilitate the inactivation of SOCE. SARAF plays a key role in shaping cytoplasmic calcium signals and determining the content of the major intracellular Ca²⁺ stores in the cell. By doing so it is likely to be important in protecting cells from calcium overfilling.^[3]

References

1. name="pmid11042152">Zhang QH, Ye M, Wu XY, Ren SX, Zhao M, Zhao CJ, Fu G, Shen Y, Fan HY, Lu G, Zhong M, Xu XR, Han ZG, Zhang JW, Tao J, Huang QH, Zhou J, Hu GX, Gu J, Chen SJ, Chen Z (Nov 2000). "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells". Genome Res 10 (10): 1546–60. doi:10.1101/gr.140200. PMC 310934. PMID 11042152.
2. "Entrez Gene: SARAF store-operated calcium entry-associated regulatory factor".
3. Palty R, Raveh A, Kaminsky I, Meller R, Reuveny E (2012). "SARAF Inactivates the Store Operated Calcium Entry Machinery to Prevent Excess Calcium Refilling". Cell 149. doi:10.1016/j.cell.2012.01.055.

Further reading

• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.". Gene 138 (1-2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library.". Gene 200 (1-2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Clark HF, Gurney AL, Abaya E, et al. (2003). "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.". Genome Res. 13 (10): 2265–70. doi:10.1101/gr.1293003. PMC 403697. PMID 12975309.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs.". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Zhang Z, Henzel WJ (2005). "Signal peptide prediction based on analysis of experimentally verified cleavage sites.". Protein Sci. 13 (10): 2819–24. doi:10.1110/ps.04682504. PMC 2286551. PMID 15340161.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Otsuki T, Ota T, Nishikawa T, et al. (2007). "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries.". DNA Res. 12 (2): 117–26. doi:10.1093/dnares/12.2.117. PMID 16303743.