TRNA pseudouridine13 synthase

TRNA pseudouridine^13 synthase
Identifiers
EC number	5.4.99.27
CAS number	430429-15-5
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

TRNA pseudouridine¹³ synthase (EC 5.4.99.27, TruD, YgbO, tRNA PSI13 synthase, RNA:PSI-synthase Pus7p, Pus7p, RNA:pseudouridine-synthase Pus7p, Pus7 protein) is an enzyme with systematic name tRNA-uridine¹³ uracil mutase.^{[1][2][3][4][5]} This enzyme catalyses the following chemical reaction

tRNA uridine¹³ $\rightleftharpoons$ tRNA pseudouridine¹³

Pseudouridine synthase TruD from Escherichia coli specifically acts on uridine¹³ in tRNA.

References

1. Ericsson, U.B., Nordlund, P. and Hallberg, B.M. (2004). "X-ray structure of tRNA pseudouridine synthase TruD reveals an inserted domain with a novel fold". FEBS Lett. 565: 59–64. doi:10.1016/j.febslet.2004.03.085. PMID 15135053.
2. Chan, C.M. and Huang, R.H. (2009). "Enzymatic characterization and mutational studies of TruD—the fifth family of pseudouridine synthases". Arch. Biochem. Biophys. 489: 15–19. doi:10.1016/j.abb.2009.07.023. PMID 19664587.
3. Kaya, Y. and Ofengand, J. (2003). "A novel unanticipated type of pseudouridine synthase with homologs in bacteria, archaea, and eukarya". RNA 9: 711–721. doi:10.1261/rna.5230603. PMID 12756329.
4. Behm-Ansmant, I., Urban, A., Ma, X., Yu, Y.T., Motorin, Y. and Branlant, C. (2003). "The Saccharomyces cerevisiae U2 snRNA:pseudouridine-synthase Pus7p is a novel multisite-multisubstrate RNA:Ψ-synthase also acting on tRNAs". RNA 9: 1371–1382. doi:10.1261/rna.5520403. PMID 14561887.
5. Urban, A., Behm-Ansmant, I., Branlant, C. and Motorin, Y. (2009). "RNA sequence and two-dimensional structure features required for efficient substrate modification by the Saccharomyces cerevisiae RNA:Ψ-synthase Pus7p". J. Biol. Chem. 284: 5845–5858. doi:10.1074/jbc.m807986200. PMID 19114708.

External links

• TRNA pseudouridine13 synthase at the US National Library of Medicine Medical Subject Headings (MeSH)