TRNA pseudouridine32 synthase

TRNA pseudouridine^32 synthase
Identifiers
EC number	5.4.99.28
CAS number	430429-15-5
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

TRNA pseudouridine³² synthase (EC 5.4.99.28, RluA, pseudouridine synthase RluA, Pus9p, Rib2/Pus8p) is an enzyme with systematic name tRNA-uridine³² uracil mutase.^{[1][2][3][4][5][6]} This enzyme catalyses the following chemical reaction

tRNA uridine³² $\rightleftharpoons$ tRNA pseudouridine³²

The dual enzyme from Escherichia coli also catalyses the formation of pseudouridine⁷⁴⁶ in 23S rRNA.

References

1. Hoang, C., Chen, J., Vizthum, C.A., Kandel, J.M., Hamilton, C.S., Mueller, E.G. and Ferre-D'Amare, A.R. (2006). "Crystal structure of pseudouridine synthase RluA: indirect sequence readout through protein-induced RNA structure". Mol. Cell 24: 535–545. doi:10.1016/j.molcel.2006.09.017. PMID 17188032.
2. Spedaliere, C.J., Hamilton, C.S. and Mueller, E.G. (2000). "Functional importance of motif I of pseudouridine synthases: mutagenesis of aligned lysine and proline residues". Biochemistry 39: 9459–9465. doi:10.1021/bi001079n. PMID 10924141.
3. Raychaudhuri, S., Niu, L., Conrad, J., Lane, B.G. and Ofengand, J. (1999). "Functional effect of deletion and mutation of the Escherichia coli ribosomal RNA and tRNA pseudouridine synthase RluA". J. Biol. Chem. 274: 18880–18886. doi:10.1074/jbc.274.27.18880. PMID 10383384.
4. Ramamurthy, V., Swann, S.L., Spedaliere, C.J. and Mueller, E.G. (1999). "Role of cysteine residues in pseudouridine synthases of different families". Biochemistry 38: 13106–13111. doi:10.1021/bi9913911. PMID 10529181.
5. Wrzesinski, J., Nurse, K., Bakin, A., Lane, B.G. and Ofengand, J. (1995). "A dual-specificity pseudouridine synthase: an Escherichia coli synthase purified and cloned on the basis of its specificity for Ψ⁷⁴⁶ in 23S RNA is also specific for Ψ³² in tRNA^Phe". RNA 1: 437–448. PMID 7493321.
6. Behm-Ansmant, I., Grosjean, H., Massenet, S., Motorin, Y. and Branlant, C. (2004). "Pseudouridylation at position 32 of mitochondrial and cytoplasmic tRNAs requires two distinct enzymes in Saccharomyces cerevisiae". J. Biol. Chem. 279: 52998–53006. doi:10.1074/jbc.m409581200. PMID 15466869.

External links

• TRNA pseudouridine32 synthase at the US National Library of Medicine Medical Subject Headings (MeSH)