TRNA pseudouridine55 synthase

TRNA pseudouridine^55 synthase
Identifiers
EC number	5.4.99.25
CAS number	430429-15-5
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

TRNA pseudouridine⁵⁵ synthase (EC 5.4.99.25, TruB, aCbf5, Pus4, YNL292w (gene), Psi55 tRNA pseudouridine synthase, tRNA:Psi⁵⁵-synthase, tRNA pseudouridine 55 synthase, tRNA:pseudouridine-55 synthase, Psi⁵⁵ synthase, tRNA Psi⁵⁵ synthase, tRNA:Psi⁵⁵ synthase, tRNA-uridine⁵⁵ uracil mutase, Pus10, tRNA-uridine^54/55 uracil mutase) is an enzyme with systematic name tRNA-uridine⁵⁵ uracil mutase.^{[1][2][3][4][5][6]} This enzyme catalyses the following chemical reaction

tRNA uridine⁵⁵ $\rightleftharpoons$ tRNA pseudouridine⁵⁵

Pseudouridine synthase TruB from Escherichia coli specifically modifies uridine⁵⁵ in tRNA molecules.

References

1. Nurse, K., Wrzesinski, J., Bakin, A., Lane, B.G. and Ofengand, J. (1995). "Purification, cloning, and properties of the tRNA Ψ⁵⁵ synthase from Escherichia coli". RNA 1: 102–112. PMID 7489483.
2. Becker, H.F., Motorin, Y., Planta, R.J. and Grosjean, H. (1997). "The yeast gene YNL292w encodes a pseudouridine synthase (Pus4) catalyzing the formation of Ψ⁵⁵ in both mitochondrial and cytoplasmic tRNAs". Nucleic Acids Res. 25: 4493–4499. doi:10.1093/nar/25.22.4493. PMID 9358157.
3. Pienkowska, J., Wrzesinski, J. and Szweykowska-Kulinska, Z. (1998). "A cell-free yellow lupin extract containing activities of pseudouridine 35 and 55 synthases". Acta Biochim. Pol. 45: 745–754. PMID 9918501.
4. Chaudhuri, B.N., Chan, S., Perry, L.J. and Yeates, T.O. (2004). "Crystal structure of the apo forms of Ψ55 tRNA pseudouridine synthase from Mycobacterium tuberculosis: a hinge at the base of the catalytic cleft". J. Biol. Chem. 279: 24585–24591. doi:10.1074/jbc.m401045200. PMID 15028724.
5. Hoang, C., Hamilton, C.S., Mueller, E.G. and Ferre-D'Amare, A.R. (2005). "Precursor complex structure of pseudouridine synthase TruB suggests coupling of active site perturbations to an RNA-sequestering peripheral protein domain". Protein Sci. 14: 2201–2206. doi:10.1110/ps.051493605. PMID 15987897.
6. Gurha, P. and Gupta, R. (2008). "Archaeal Pus10 proteins can produce both pseudouridine 54 and 55 in tRNA". RNA 14: 2521–2527. doi:10.1261/rna.1276508. PMID 18952823.

External links

• TRNA pseudouridine55 synthase at the US National Library of Medicine Medical Subject Headings (MeSH)