Toluidine blue stain

Toluidine blue stain in a vasculitic peripheral neuropathy.

Toluidine blue is a basic thiazine metachromatic dye with high affinity for acidic tissue components.[1]

It stains nucleic acids blue and polysaccharides purple and also increases the sharpness of histology slide images. It is especially useful today for staining chromosomes in plant or animal tissues, as a replacement for Aceto-orcein stain.

Results

The results depend on the studied organs:[2]

- Mastocytes in purple

- Cartilage in purple

- Mucins in purple/red

- Nuclei in blue

It is used in forensic examination,[3] renal pathology[4] and neuropathology.

See also

References

  1. ↑ http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3424943/
  2. ↑ http://www.histalim.com/accueil/activities/our-services/histology/toluidine-blue/
  3. ↑ Olshaker, Jackson and Smock (2001). Forensic Emergency Medicine. Philadelphia: Lippincott, Williams and Williams. pp. 94–97. ISBN 0781731445.
  4. ↑ Nicholas, SB.; Basgen, JM.; Sinha, S. (2011). "Using stereologic techniques for podocyte counting in the mouse: shifting the paradigm.". Am J Nephrol. 33 Suppl 1: 1–7. doi:10.1159/000327564. PMC 3121548. PMID 21659728.

External links

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