Trans-o-hydroxybenzylidenepyruvate hydratase-aldolase

Trans-o-hydroxybenzylidenepyruvate hydratase-aldolase (EC 4.1.2.45, 2'-hydroxybenzalpyruvate aldolase, NsaE, tHBPA hydratase-aldolase) is an enzyme with systematic name (3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate hydro-lyase.^[1]^[2]^[3]^[4] This enzyme catalyses the following chemical reaction

(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate + H₂O

\rightleftharpoons

salicylaldehyde + pyruvate

This enzyme is involved in naphthalene degradation.

References

↑ Kuhm, A.E., Knackmuss, H.J. and Stolz, A. (1993). "Purification and properties of 2′-hydroxybenzalpyruvate aldolase from a bacterium that degrades naphthalenesulfonates". J. Biol. Chem. 268 (13): 9484–9489. PMID 8486638.
↑ Keck, A., Conradt, D., Mahler, A., Stolz, A., Mattes, R. and Klein, J. (2006). "Identification and functional analysis of the genes for naphthalenesulfonate catabolism by Sphingomonas xenophaga BN6". Microbiology 152: 1929–1940. doi:10.1099/mic.0.28783-0. PMID 16804169.
↑ Eaton, R.W. (1994). "Organization and evolution of naphthalene catabolic pathways: sequence of the DNA encoding 2-hydroxychromene-2-carboxylate isomerase and trans-o-hydroxybenzylidenepyruvate hydratase-aldolase from the NAH7 plasmid". J. Bacteriol. 176 (24): 7757–7762. PMID 8002605.
↑ Eaton, R.W. (2000). "trans-o-Hydroxybenzylidenepyruvate hydratase-aldolase as a biocatalyst". Appl. Environ. Microbiol. 66 (6): 2668–2672. doi:10.1128/aem.66.6.2668-2672.2000. PMID 10831455.

External links

Trans-o-hydroxybenzylidenepyruvate hydratase-aldolase at the US National Library of Medicine Medical Subject Headings (MeSH)

Carbon-carbon lyases (EC 4.1)

4.1.1: Carboxy-lyases	Acetoacetate decarboxylase Adenosylmethionine decarboxylase Aromatic L-amino acid decarboxylase Glutamate decarboxylase Histidine decarboxylase Lysine decarboxylase Malonyl-CoA decarboxylase Ornithine decarboxylase Oxaloacetate decarboxylase Phosphoenolpyruvate carboxykinase Phosphoenolpyruvate carboxylase Phosphoribosylaminoimidazole carboxylase Pyrophosphomevalonate decarboxylase Pyruvate decarboxylase RuBisCO Uridine monophosphate synthetase/Orotidine 5'-phosphate decarboxylase Uroporphyrinogen III decarboxylase

4.1.2: Aldehyde-lyases	Fructose-bisphosphate aldolase Aldolase A Aldolase B Aldolase C 2-hydroxyphytanoyl-CoA lyase Threonine aldolase

4.1.3: Oxo-acid-lyases	Isocitrate lyase 3-hydroxy-3-methylglutaryl-CoA lyase

4.1.99: Other	Tryptophanase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases(list) EC2 Transferases(list) EC3 Hydrolases(list) EC4 Lyases(list) EC5 Isomerases(list) EC6 Ligases(list)

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