ZFP36

ZFP36 ring finger protein
PDB rendering based on 1m9o.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 4J8S​
Identifiers
Symbols	ZFP36 ; G0S24; GOS24; NUP475; RNF162A; TIS11; TTP; zfp-36
External IDs	OMIM: 190700 MGI: 99180 HomoloGene: 2558 GeneCards: ZFP36 Gene
Gene ontology Molecular function • DNA binding • single-stranded RNA binding • mRNA binding • protein binding • AU-rich element binding • enzyme binding • protein kinase binding • C-C chemokine binding • mRNA 3'-UTR AU-rich region binding • poly(A) RNA binding • metal ion binding • 14-3-3 protein binding Cellular component • nucleus • cytoplasm • cytosol • cytoplasmic stress granule Biological process • negative regulation of transcription from RNA polymerase II promoter • nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay • nuclear-transcribed mRNA poly(A) tail shortening • mRNA catabolic process • gene expression • regulation of tumor necrosis factor production • negative regulation of translation involved in gene silencing by miRNA • response to starvation • positive regulation of nuclear-transcribed mRNA poly(A) tail shortening • 3'-UTR-mediated mRNA stabilization • positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	7538	22695
Ensembl	ENSG00000128016	ENSMUSG00000044786
UniProt	P26651	P22893
RefSeq (mRNA)	NM_003407	NM_011756
RefSeq (protein)	NP_003398	NP_035886
Location (UCSC)	Chr 19: 39.41 – 39.41 Mb	Chr 7: 28.38 – 28.38 Mb
PubMed search

Tristetraprolin (TTP), also known as zinc finger protein 36 homolog (ZFP36), is a protein that in humans, mice and rats is encoded by the ZFP36 gene.^[1][2] It is a member of the TIS11 (TPA-induced sequence) family, along with butyrate response factors 1 and 2.^[3]

TTP binds to AU-rich elements (AREs) in the 3'-untranslated regions (UTRs) of the mRNAs of some cytokines and promotes their degradation. For example, TTP is a component of a negative feedback loop that interferes with TNF-alpha production by destabilizing its mRNA.^[4] Mice deficient in TTP develop a complex syndrome of inflammatory diseases.^[4]

Interactions

ZFP36 has been shown to interact with 14-3-3 protein family members, such as YWHAH,^[5] and with NUP214, a member of the nuclear pore complex.^[6]

Regulation

Post-transcriptionally, TTP is regulated in several ways.^[3] The subcellular localization of TTP is influenced by interactions with protein partners such as the 14-3-3 family of proteins. These interactions and, possibly, interactions with target mRNAs are affected by the phosphorylation state of TTP, as the protein can be posttranslationally modified by a large number of protein kinases.^[3] There is some evidence that the TTP transcript may also be targeted by microRNAs, such as miR-29a.^[3]

References

1. DuBois RN, McLane MW, Ryder K, Lau LF, Nathans D (Dec 1990). "A growth factor-inducible nuclear protein with a novel cysteine/histidine repetitive sequence". J Biol Chem 265 (31): 19185–91. PMID 1699942.
2. "Entrez Gene: ZFP36 zinc finger protein 36, C3H type, homolog (mouse)".
3. Sanduja S, Blanco FF, Dixon DA (2011). "The roles of TTP and BRF proteins in regulated mRNA decay". Wiley Interdiscip Rev RNA 2 (1): 42–57. doi:10.1002/wrna.28. PMC 3030256. PMID 21278925.
4. Carballo E, Lai WS, Blackshear PJ (August 1998). "Feedback inhibition of macrophage tumor necrosis factor-alpha production by tristetraprolin". Science 281 (5379): 1001–5. doi:10.1126/science.281.5379.1001. PMID 9703499.
5. Johnson BA, Stehn JR, Yaffe MB, Blackwell TK (May 2002). "Cytoplasmic localization of tristetraprolin involves 14-3-3-dependent and -independent mechanisms". J. Biol. Chem. 277 (20): 18029–36. doi:10.1074/jbc.M110465200. PMID 11886850.
6. Carman JA, Nadler SG (March 2004). "Direct association of tristetraprolin with the nucleoporin CAN/Nup214". Biochem. Biophys. Res. Commun. 315 (2): 445–9. doi:10.1016/j.bbrc.2004.01.080. PMID 14766228.

Further reading

• Blackshear PJ (2003). "Tristetraprolin and other CCCH tandem zinc-finger proteins in the regulation of mRNA turnover". Biochem. Soc. Trans. 30 (Pt 6): 945–52. doi:10.1042/bst0300945. PMID 12440952.
• Carrick DM, Lai WS, Blackshear PJ (2005). "The tandem CCCH zinc finger protein tristetraprolin and its relevance to cytokine mRNA turnover and arthritis". Arthritis Research & Therapy 6 (6): 248–64. doi:10.1186/ar1441. PMC 1064869. PMID 15535838.
• Taylor GA, Lai WS, Oakey RJ, et al. (1991). "The human TTP protein: sequence, alignment with related proteins, and chromosomal localization of the mouse and human genes". Nucleic Acids Res. 19 (12): 3454. doi:10.1093/nar/19.12.3454. PMC 328350. PMID 2062660.
• Lai WS, Stumpo DJ, Blackshear PJ (1990). "Rapid insulin-stimulated accumulation of an mRNA encoding a proline-rich protein". J. Biol. Chem. 265 (27): 16556–63. PMID 2204625.
• Taylor GA, Thompson MJ, Lai WS, Blackshear PJ (1995). "Phosphorylation of tristetraprolin, a potential zinc finger transcription factor, by mitogen stimulation in intact cells and by mitogen-activated protein kinase in vitro". J. Biol. Chem. 270 (22): 13341–7. doi:10.1074/jbc.270.22.13341. PMID 7768935.
• Heximer SP, Forsdyke DR (1993). "A human putative lymphocyte G0/G1 switch gene homologous to a rodent gene encoding a zinc-binding potential transcription factor". DNA Cell Biol. 12 (1): 73–88. doi:10.1089/dna.1993.12.73. PMID 8422274.
• Huebner K, Druck T, LaForgia S, et al. (1993). "Chromosomal localization of four human zinc finger cDNAs". Hum. Genet. 91 (3): 217–22. doi:10.1007/BF00218259. PMID 8478004.
• Lai WS, Carballo E, Thorn JM, et al. (2000). "Interactions of CCCH zinc finger proteins with mRNA. Binding of tristetraprolin-related zinc finger proteins to Au-rich elements and destabilization of mRNA". J. Biol. Chem. 275 (23): 17827–37. doi:10.1074/jbc.M001696200. PMID 10751406.
• Dintilhac A, Bernués J (2002). "HMGB1 interacts with many apparently unrelated proteins by recognizing short amino acid sequences". J. Biol. Chem. 277 (9): 7021–8. doi:10.1074/jbc.M108417200. PMID 11748221.
• Lai WS, Kennington EA, Blackshear PJ (2002). "Interactions of CCCH zinc finger proteins with mRNA: non-binding tristetraprolin mutants exert an inhibitory effect on degradation of AU-rich element-containing mRNAs". J. Biol. Chem. 277 (11): 9606–13. doi:10.1074/jbc.M110395200. PMID 11782475.
• Johnson BA, Stehn JR, Yaffe MB, Blackwell TK (2002). "Cytoplasmic localization of tristetraprolin involves 14-3-3-dependent and -independent mechanisms". J. Biol. Chem. 277 (20): 18029–36. doi:10.1074/jbc.M110465200. PMID 11886850.
• Brooks SA, Connolly JE, Diegel RJ, et al. (2002). "Analysis of the function, expression, and subcellular distribution of human tristetraprolin". Arthritis Rheum. 46 (5): 1362–70. doi:10.1002/art.10235. PMID 12115244.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Amann BT, Worthington MT, Berg JM (2003). "A Cys3His zinc-binding domain from Nup475/tristetraprolin: a novel fold with a disklike structure". Biochemistry 42 (1): 217–21. doi:10.1021/bi026988m. PMID 12515557.
• Yu H, Stasinopoulos S, Leedman P, Medcalf RL (2003). "Inherent instability of plasminogen activator inhibitor type 2 mRNA is regulated by tristetraprolin". J. Biol. Chem. 278 (16): 13912–8. doi:10.1074/jbc.M213027200. PMID 12578825.
• Sawaoka H, Dixon DA, Oates JA, Boutaud O (2003). "Tristetraprolin binds to the 3'-untranslated region of cyclooxygenase-2 mRNA. A polyadenylation variant in a cancer cell line lacks the binding site". J. Biol. Chem. 278 (16): 13928–35. doi:10.1074/jbc.M300016200. PMID 12578839.