1-pyrroline-4-hydroxy-2-carboxylate deaminase

1-pyrroline-4-hydroxy-2-carboxylate deaminase
Identifiers
EC number	3.5.4.22
CAS number	9054-77-7
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / EGO
Search PMC articles PubMed articles NCBI proteins

In enzymology, a 1-pyrroline-4-hydroxy-2-carboxylate deaminase (EC 3.5.4.22) is an enzyme that catalyzes the chemical reaction

1-pyrroline-4-hydroxy-2-carboxylate + H₂O $\rightleftharpoons$ 2,5-dioxopentanoate + NH₃

Thus, the two substrates of this enzyme are 1-pyrroline-4-hydroxy-2-carboxylate and H₂O, whereas its two products are 2,5-dioxopentanoate and NH₃.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in cyclic amidines. The systematic name of this enzyme class is 1-pyrroline-4-hydroxy-2-carboxylate aminohydrolase (decyclizing). This enzyme is also called HPC deaminase. This enzyme participates in arginine and proline metabolism.

References

• Singh RM, Adams E (1965). "Enzymatic deamination of delta-1-pyrroline-4-hydroxy-2-carboxylate to 2,5-dioxovalerate (alpha-ketoglutaric semialdehyde)". The Journal of Biological Chemistry 240 (11): 4344–51. PMID 5845838.
• Singh RM, Adams E (Nov 1965). "Isolation and identification of 2,5-dioxovalerate, an intermediate in the bacterial oxidation of hydroxyproline". The Journal of Biological Chemistry 240 (11): 4352–6. PMID 5845839.