Inosine monophosphate synthase

5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase
PDB rendering based on 1p4r.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1P4R​, 1PKX​, 1PL0​
Identifiers
Symbols	ATIC ; AICAR; AICARFT; HEL-S-70p; IMPCHASE; PURH
External IDs	OMIM: 601731 MGI: 1351352 HomoloGene: 2983 ChEMBL: 2518 GeneCards: ATIC Gene
EC number	2.1.2.3, 3.5.4.10
Gene ontology Molecular function • IMP cyclohydrolase activity • phosphoribosylaminoimidazolecarboxamide formyltransferase activity • protein homodimerization activity Cellular component • mitochondrion • cytosol • membrane • extracellular exosome Biological process • brainstem development • nucleobase-containing compound metabolic process • purine nucleobase metabolic process • 'de novo' IMP biosynthetic process • nucleoside metabolic process • purine ribonucleoside monophosphate biosynthetic process • response to inorganic substance • cerebellum development • cerebral cortex development • organ regeneration • small molecule metabolic process • dihydrofolate metabolic process • tetrahydrofolate biosynthetic process • nucleobase-containing small molecule metabolic process Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	471	108147
Ensembl	ENSG00000138363	ENSMUSG00000026192
UniProt	P31939	Q9CWJ9
RefSeq (mRNA)	NM_004044	NM_026195
RefSeq (protein)	NP_004035	NP_080471
Location (UCSC)	Chr 2: 215.31 – 215.35 Mb	Chr 1: 71.56 – 71.58 Mb
PubMed search

Bifunctional purine biosynthesis protein PURH is a protein that in humans is encoded by the ATIC gene.^[1][2][3] ATIC encodes an enzyme which generates inosine monophosphate from aminoimidazole carboxamide ribonucleotide.

It has two functions:

• EC 2.1.2.3 - 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase
• EC 3.5.4.10 - IMP cyclohydrolase

References

1. Rayl EA, Moroson BA, Beardsley GP (Mar 1996). "The human purH gene product, 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase. Cloning, sequencing, expression, purification, kinetic analysis, and domain mapping". J Biol Chem 271 (4): 2225–33. doi:10.1074/jbc.271.4.2225. PMID 8567683.
2. Sugita T, Aya H, Ueno M, Ishizuka T, Kawashima K (Nov 1997). "Characterization of molecularly cloned human 5-aminoimidazole-4-carboxamide ribonucleotide transformylase". J Biochem 122 (2): 309–13. doi:10.1093/oxfordjournals.jbchem.a021754. PMID 9378707.
3. "Entrez Gene: ATIC 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase".

Further reading

• Rasmussen HH, van Damme J, Puype M, et al. (1993). "Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes". Electrophoresis 13 (12): 960–9. doi:10.1002/elps.11501301199. PMID 1286667.
• Yamauchi M, Seki N, Mita K, et al. (1996). "Isolation of human purH gene expressed in the rodent transformant cells by subtractive enrichment of 3'-untranslated region of human transcript". DNA Res. 2 (6): 269–75. doi:10.1093/dnares/2.6.269. PMID 8867801.
• Beardsley GP, Rayl EA, Gunn K, et al. (1998). "Structure and functional relationships in human pur H". Adv. Exp. Med. Biol. Advances in Experimental Medicine and Biology 431: 221–6. doi:10.1007/978-1-4615-5381-6_43. ISBN 978-0-306-45778-4. PMID 9598063.
• Vergis JM, Bulock KG, Fleming KG, Beardsley GP (2001). "Human 5-aminoimidazole-4-carboxamide ribonucleotide transformylase/inosine 5'-monophosphate cyclohydrolase. A bifunctional protein requiring dimerization for transformylase activity but not for cyclohydrolase activity". J. Biol. Chem. 276 (11): 7727–33. doi:10.1074/jbc.M009940200. PMID 11096114.
• Bulock KG, Beardsley GP, Anderson KS (2002). "The kinetic mechanism of the human bifunctional enzyme ATIC (5-amino-4-imidazolecarboxamide ribonucleotide transformylase/inosine 5'-monophosphate cyclohydrolase). A surprising lack of substrate channeling". J. Biol. Chem. 277 (25): 22168–74. doi:10.1074/jbc.M111964200. PMID 11948179.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Cheong CG, Wolan DW, Greasley SE, et al. (2004). "Crystal structures of human bifunctional enzyme aminoimidazole-4-carboxamide ribonucleotide transformylase/IMP cyclohydrolase in complex with potent sulfonyl-containing antifolates". J. Biol. Chem. 279 (17): 18034–45. doi:10.1074/jbc.M313691200. PMID 14966129.
• Marie S, Heron B, Bitoun P, et al. (2004). "AICA-Ribosiduria: A Novel, Neurologically Devastating Inborn Error of Purine Biosynthesis Caused by Mutation of ATIC". Am. J. Hum. Genet. 74 (6): 1276–81. doi:10.1086/421475. PMC 1182092. PMID 15114530.
• Dervieux T, Furst D, Lein DO, et al. (2004). "Polyglutamation of methotrexate with common polymorphisms in reduced folate carrier, aminoimidazole carboxamide ribonucleotide transformylase, and thymidylate synthase are associated with methotrexate effects in rheumatoid arthritis". Arthritis Rheum. 50 (9): 2766–74. doi:10.1002/art.20460. PMID 15457444.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein–protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.

External links

• Inosine monophosphate synthase at the US National Library of Medicine Medical Subject Headings (MeSH)