16S rRNA (adenine1408-N1)-methyltransferase

16S rRNA (adenine¹⁴⁰⁸-N¹)-methyltransferase (EC 2.1.1.180, kanamycin-apramycin resistance methylase, 16S rRNA:m1A1408 methyltransferase, KamB, NpmA, 16S rRNA m1A1408 methyltransferase) is an enzyme with systematic name S-adenosyl-L-methionine:16S rRNA (adenine¹⁴⁰⁸-N¹)-methyltransferase.^[1]^[2]^[3]^[4] This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + adenine¹⁴⁰⁸ in 16S rRNA

\rightleftharpoons

S-adenosyl-L-homocysteine + N¹-methyladenine¹⁴⁰⁸ in 16S rRNA

The enzyme provides a panaminoglycoside resistance through interference with the binding of aminoglycosides.

References

↑ Beauclerk, A.A. and Cundliffe, E. (1987). "Sites of action of two ribosomal RNA methylases responsible for resistance to aminoglycosides". J. Mol. Biol. 193: 661–671. doi:10.1016/0022-2836(87)90349-4. PMID 2441068.
↑ Koscinski, L., Feder, M. and Bujnicki, J.M. (2007). "Identification of a missing sequence and functionally important residues of 16S rRNA:m¹A¹⁴⁰⁸ methyltransferase KamB that causes bacterial resistance to aminoglycoside antibiotics". Cell Cycle 6: 1268–1271. doi:10.4161/cc.6.10.4231. PMID 17495534.
↑ Holmes, D.J., Drocourt, D., Tiraby, G. and Cundliffe, E. (1991). "Cloning of an aminoglycoside-resistance-encoding gene, kamC, from Saccharopolyspora hirsuta: comparison with kamB from Streptomyces tenebrarius". Gene 102: 19–26. doi:10.1016/0378-1119(91)90532-g. PMID 1840536.
↑ Wachino, J., Shibayama, K., Kurokawa, H., Kimura, K., Yamane, K., Suzuki, S., Shibata, N., Ike, Y. and Arakawa, Y. (2007). "Novel plasmid-mediated 16S rRNA m¹A¹⁴⁰⁸ methyltransferase, NpmA, found in a clinically isolated Escherichia coli strain resistant to structurally diverse aminoglycosides". Antimicrob. Agents Chemother. 51: 4401–4409. doi:10.1128/aac.00926-07. PMID 17875999.

External links

16S rRNA (adenine1408-N1)-methyltransferase at the US National Library of Medicine Medical Subject Headings (MeSH)

Transferase: one carbon transferases (EC 2.1)

2.1.1: Methyl-

N-	Histamine N-methyltransferase Phenylethanolamine N-methyltransferase Amine N-methyltransferase Phosphatidylethanolamine N-methyltransferase

O-	5-hydroxyindole-O-methyltransferase/Acetylserotonin O-methyltransferase Catechol-O-methyl transferase

Homocysteine	Betaine-homocysteine methyltransferase Homocysteine methyltransferase Methionine synthase

Other	Phosphatidyl ethanolamine methyltransferase DNMT3B Histone methyltransferase Thymidylate synthase DNA methyltransferase Thiopurine methyltransferase

2.1.2: Hydroxymethyl-,
Formyl- and Related

Hydroxymethyltransferase	Serine hydroxymethyltransferase 3-methyl-2-oxobutanoate hydroxymethyltransferase

Formyltransferase	Phosphoribosylglycinamide formyltransferase Inosine monophosphate synthase

Other	Glutamate formimidoyltransferase Aminomethyltransferase

2.1.3: Carboxy-
and Carbamoyl

Carboxy	methylmalonyl-CoA carboxytransferase

Carbamoyl	Aspartate carbamoyltransferase Ornithine carbamoyltransferase Oxamate carbamoyltransferase Putrescine carbamoyltransferase 3-hydroxymethylcephem carbamoyltransferase Lysine carbamoyltransferase N-acetylornithine carbamoyltransferase

2.1.4: Amidine

Arginine:glycine amidinotransferase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases(list) EC2 Transferases(list) EC3 Hydrolases(list) EC4 Lyases(list) EC5 Isomerases(list) EC6 Ligases(list)

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