2,5-diketocamphane 1,2-monooxygenase

2,5-diketocamphane 1,2-monooxygenase
Identifiers
EC number	1.14.13.162
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

2,5-diketocamphane 1,2-monooxygenase (EC 1.14.13.162, 2,5-diketocamphane lactonizing enzyme, ketolactonase I, 2,5-diketocamphane 1,2-monooxygenase oxygenating component, 2,5-DKCMO, camP (gene), camphor 1,2-monooxygenase, camphor ketolactonase I) is an enzyme with systematic name (+)-bornane-2,5-dione,NADH:oxygen oxidoreductase (1,2-lactonizing).^{[1][2][3][4][5][6]} This enzyme catalyses the following chemical reaction

(+)-bornane-2,5-dione + O₂ + NADH + H⁺ $\rightleftharpoons$ (+)-5-oxo-1,2-campholide + NAD⁺ + H₂O

2,5-diketocamphane 1,2-monooxygenase is a flavoprotein (FMN) which requires Fe²⁺.

References

1. Conrad, H.E., DuBus, R., Namtvedt, M.J. and Gunsalus, I.C. (1965). "Mixed function oxidation. II. Separation and properties of the enzymes catalyzing camphor lactonizaton". J. Biol. Chem. 240: 495–503. PMID 14253460.
2. Yu, C.A. and Gunsalus, I.C. (1969). "Monoxygenases. VII. Camphor ketolactonase I and the role of three protein components". J. Biol. Chem. 244 (22): 6149–6152. PMID 4310834.
3. Taylor, D.G. and Trudgill, P.W. (1986). "Camphor revisited: studies of 2,5-diketocamphane 1,2-monooxygenase from Pseudomonas putida ATCC 17453". J. Bacteriol. 165 (2): 489–497. PMC 214445. PMID 3944058.
4. Selifonov, S.A. (1992). "Microbial oxidation of adamantanone by Pseudomonas putida carrying the camphor catabolic plasmid". Biochem. Biophys. Res. Commun. 186 (3): 1429–1436. doi:10.1016/s0006-291x(05)81566-9. PMID 1510672.
5. Jones, K.H., Smith, R.T. and Trudgill, P.W. (1993). "Diketocamphane enantiomer-specific ’Baeyer-Villiger’ monooxygenases from camphor-grown Pseudomonas putida ATCC 17453". J. Gen. Microbiol. 139 (4): 797–805. doi:10.1099/00221287-139-4-797. PMID 8515237.
6. Kadow, M., Sass, S., Schmidt, M. and Bornscheuer, U.T. (2011). "Recombinant expression and purification of the 2,5-diketocamphane 1,2-monooxygenase from the camphor metabolizing Pseudomonas putida strain NCIMB 10007". AMB Express 1 (1): #13–13. doi:10.1186/2191-0855-1-13. PMC 3222318. PMID 21906366.

External links

• 2,5-diketocamphane 1,2-monooxygenase at the US National Library of Medicine Medical Subject Headings (MeSH)