Isoflavonoid synthase

Isoflavonoid synthase
Identifiers
EC number	1.14.13.136
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

2-hydroxyisoflavanone synthase (EC 1.14.13.136, CYT93C, IFS, isoflavonoid synthase) is an enzyme with systematic name liquiritigenin,NADPH:oxygen oxidoreductase (hydroxylating, aryl migration).^[1][2][3] This enzyme catalyses the following chemical reactions:

liquiritigenin + O₂ + NADPH + H⁺ $\rightleftharpoons$ 2,4',7-trihydroxyisoflavanone + H₂O + NADP⁺

and

(2S)-naringenin + O₂ + NADPH + H⁺ $\rightleftharpoons$ 2,4',5,7-tetrahydroxyisoflavanone + H₂O + NADP⁺

Isoflavonoid synthase requires cytochrome P450.

References

1. Hashim, M.F., Hakamatsuka, T., Ebizuka, Y. and Sankawa, U. (1990). "Reaction mechanism of oxidative rearrangement of flavanone in isoflavone biosynthesis". FEBS Lett. 271 (1-2): 219–222. doi:10.1016/0014-5793(90)80410-k. PMID 2226805.
2. Sawada, Y., Kinoshita, K., Akashi, T., Aoki, T. and Ayabe, S. (2002). "Key amino acid residues required for aryl migration catalysed by the cytochrome P450 2-hydroxyisoflavanone synthase". Plant J. 31 (5): 555–564. doi:10.1046/j.1365-313x.2002.01378.x. PMID 12207646.
3. Sawada, Y. and Ayabe, S. (2005). "Multiple mutagenesis of P₄₅₀ isoflavonoid synthase reveals a key active-site residue". Biochem. Biophys. Res. Commun. 330 (3): 907–913. doi:10.1016/j.bbrc.2005.03.053. PMID 15809082.

External links

• Isoflavonoid synthase at the US National Library of Medicine Medical Subject Headings (MeSH)