2-oxoglutaramate amidase

2-oxoglutaramate amidase (EC 3.5.1.111, omega-amidase) is an enzyme with systematic name 5-amino-2,5-dioxopentanoate amidohydrolase.^[1] This enzyme catalyses the following chemical reaction

2-oxoglutaramate + H₂O

\rightleftharpoons

2-oxoglutarate + ammonia

The enzyme participates in the nicotine degradation pathway of several Gram-positive bacteria.

References

↑ Cobzaru, C., Ganas, P., Mihasan, M., Schleberger, P. and Brandsch, R. (2011). "Homologous gene clusters of nicotine catabolism, including a new ω-amidase for α-ketoglutaramate, in species of three genera of Gram-positive bacteria". Res. Microbiol. 162: 285–291. doi:10.1016/j.resmic.2011.01.001. PMID 21288482.

External links

2-oxoglutaramate amidase at the US National Library of Medicine Medical Subject Headings (MeSH)

Hydrolases: carbon-nitrogen non-peptide (EC 3.5)

3.5.1: Linear amides / Amidohydrolases	Asparaginase Glutaminase Urease Biotinidase Aspartoacylase Ceramidase Aspartylglucosaminidase Fatty acid amide hydrolase Histone deacetylase Sirtuin

3.5.2: Cyclic amides/ Amidohydrolases	Barbiturase Beta-lactamase

3.5.3: Linear amidines/ Ureohydrolases	Arginase Agmatinase Protein-arginine deiminase

3.5.4: Cyclic amidines/ Aminohydrolases	Guanine deaminase Adenosine deaminase AMP deaminase Inosine monophosphate synthase DCMP deaminase GTP cyclohydrolase I Cytidine deaminase AICDA Activation-induced cytidine deaminase

3.5.5: Nitriles/ Aminohydrolases	Nitrilase

3.5.99: Other	Riboflavinase Thiaminase II

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases(list) EC2 Transferases(list) EC3 Hydrolases(list) EC4 Lyases(list) EC5 Isomerases(list) EC6 Ligases(list)

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