23S rRNA (adenine2085-N6)-dimethyltransferase

23S rRNA (adenine²⁰⁸⁵-N⁶)-dimethyltransferase (EC 2.1.1.184, ErmC' methyltransferase, ermC methylase, ermC 23S rRNA methyltransferase, rRNA:m⁶A methyltransferase ErmC', ErmC', rRNA methyltransferase ErmC' ) is an enzyme with systematic name S-adenosyl-L-methionine:23S rRNA (adenine²⁰⁸⁵-N⁶)-dimethyltransferase.^[1]^[2]^[3]^[4]^[5]^[6] This enzyme catalyses the following chemical reaction

2 S-adenosyl-L-methionine + adenine²⁰⁸⁵ in 23S rRNA

\rightleftharpoons

2 S-adenosyl-L-homocysteine + N⁶-dimethyladenine²⁰⁸⁵ in 23S rRNA

ErmC is a methyltransferase that confers resistance to the macrolide-lincosamide-streptogramin B group of antibiotics by catalysing the methylation of 23S rRNA at adenine²⁰⁸⁵.

References

↑ Zhong, P., Pratt, S.D., Edalji, R.P., Walter, K.A., Holzman, T.F., Shivakumar, A.G. and Katz, L. (1995). "Substrate requirements for ErmC′ methyltransferase activity". J. Bacteriol. 177: 4327–4332. PMID 7543473.
↑ Denoya, C. and Dubnau, D. (1989). "Mono- and dimethylating activities and kinetic studies of the ermC 23 S rRNA methyltransferase". J. Biol. Chem. 264: 2615–2624. PMID 2492520.
↑ Denoya, C.D. and Dubnau, D. (1987). "Site and substrate specificity of the ermC 23S rRNA methyltransferase". J. Bacteriol. 169: 3857–3860. PMID 2440853.
↑ Bussiere, D.E., Muchmore, S.W., Dealwis, C.G., Schluckebier, G., Nienaber, V.L., Edalji, R.P., Walter, K.A., Ladror, U.S., Holzman, T.F. and Abad-Zapatero, C. (1998). "Crystal structure of ErmC′, an rRNA methyltransferase which mediates antibiotic resistance in bacteria". Biochemistry 37: 7103–7112. doi:10.1021/bi973113c. PMID 9585521.
↑ Schluckebier, G., Zhong, P., Stewart, K.D., Kavanaugh, T.J. and Abad-Zapatero, C. (1999). "The 2.2 Å structure of the rRNA methyltransferase ErmC′ and its complexes with cofactor and cofactor analogs: implications for the reaction mechanism". J. Mol. Biol. 289: 277–291. doi:10.1006/jmbi.1999.2788. PMID 10366505.
↑ Maravic, G., Bujnicki, J.M., Feder, M., Pongor, S. and Flogel, M. (2003). "Alanine-scanning mutagenesis of the predicted rRNA-binding domain of ErmC′ redefines the substrate-binding site and suggests a model for protein-RNA interactions". Nucleic Acids Res. 31: 4941–4949. doi:10.1093/nar/gkg666. PMID 12907737.

External links

23S rRNA (adenine2085-N6)-dimethyltransferase at the US National Library of Medicine Medical Subject Headings (MeSH)

Transferase: one carbon transferases (EC 2.1)

2.1.1: Methyl-

N-	Histamine N-methyltransferase Phenylethanolamine N-methyltransferase Amine N-methyltransferase Phosphatidylethanolamine N-methyltransferase

O-	5-hydroxyindole-O-methyltransferase/Acetylserotonin O-methyltransferase Catechol-O-methyl transferase

Homocysteine	Betaine-homocysteine methyltransferase Homocysteine methyltransferase Methionine synthase

Other	Phosphatidyl ethanolamine methyltransferase DNMT3B Histone methyltransferase Thymidylate synthase DNA methyltransferase Thiopurine methyltransferase

2.1.2: Hydroxymethyl-,
Formyl- and Related

Hydroxymethyltransferase	Serine hydroxymethyltransferase 3-methyl-2-oxobutanoate hydroxymethyltransferase

Formyltransferase	Phosphoribosylglycinamide formyltransferase Inosine monophosphate synthase

Other	Glutamate formimidoyltransferase Aminomethyltransferase

2.1.3: Carboxy-
and Carbamoyl

Carboxy	methylmalonyl-CoA carboxytransferase

Carbamoyl	Aspartate carbamoyltransferase Ornithine carbamoyltransferase Oxamate carbamoyltransferase Putrescine carbamoyltransferase 3-hydroxymethylcephem carbamoyltransferase Lysine carbamoyltransferase N-acetylornithine carbamoyltransferase

2.1.4: Amidine

Arginine:glycine amidinotransferase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases(list) EC2 Transferases(list) EC3 Hydrolases(list) EC4 Lyases(list) EC5 Isomerases(list) EC6 Ligases(list)

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