23S rRNA (adenine2503-C2)-methyltransferase

23S rRNA (adenine²⁵⁰³-C²)-methyltransferase (EC 2.1.1.192, RlmN, YfgB, Cfr) is an enzyme with systematic name S-adenosyl-L-methionine:23S rRNA (adenine²⁵⁰³-C²)-methyltransferase.^[1]^[2]^[3]^[4]^[5] This enzyme catalyses the following chemical reaction

2 S-adenosyl-L-methionine + adenine²⁵⁰³ in 23S rRNA

\rightleftharpoons

^{S-adenosyl-L-homocysteine} + L-methionine + 5'-deoxyadenosine + 2-methyladenine²⁵⁰³ in 23S rRNA

23S rRNA (adenine²⁵⁰³-C²)-methyltransferase contains an [4Fe-4S] cluster.

References

↑ Toh, S.M., Xiong, L., Bae, T. and Mankin, A.S. (2008). "The methyltransferase YfgB/RlmN is responsible for modification of adenosine 2503 in 23S rRNA". RNA 14 (1): 98–106. doi:10.1261/rna.814408. PMC 2151032. PMID 18025251.
↑ Yan, F., LaMarre, J.M., RÃ¶hrich, R., Wiesner, J., Jomaa, H., Mankin, A.S., Fujimori, D.G. (2010). "RlmN and Cfr are radical SAM enzymes involved in methylation of ribosomal RNA". J. Am. Chem. Soc. 132 (11): 3953–3964. doi:10.1021/ja910850y. PMC 2859901. PMID 20184321.
↑ Yan, F. and Fujimori, D.G. (2011). "RNA methylation by Radical SAM enzymes RlmN and Cfr proceeds via methylene transfer and hydride shift". Proc. Natl. Acad. Sci. USA 108 (10): 3930–3934. doi:10.1073/pnas.1017781108. PMC 3054002. PMID 21368151.
↑ Grove, T.L., Benner, J.S., Radle, M.I., Ahlum, J.H., Landgraf, B.J., Krebs, C. and Booker, S.J. (2011). "A radically different mechanism for S-adenosylmethionine-dependent methyltransferases". Science 332 (6029): 604–607. doi:10.1126/science.1200877. PMID 21415317.
↑ Boal, A.K., Grove, T.L., McLaughlin, M.I., Yennawar, N.H., Booker, S.J. and Rosenzweig, A.C. (2011). "Structural basis for methyl transfer by a radical SAM enzyme". Science 332 (6033): 544–545. doi:10.1126/science.1205358. PMID 21527678.

External links

23S rRNA (adenine2503-C2)-methyltransferase at the US National Library of Medicine Medical Subject Headings (MeSH)

Transferase: one carbon transferases (EC 2.1)

2.1.1: Methyl-

N-	Histamine N-methyltransferase Phenylethanolamine N-methyltransferase Amine N-methyltransferase Phosphatidylethanolamine N-methyltransferase

O-	5-hydroxyindole-O-methyltransferase/Acetylserotonin O-methyltransferase Catechol-O-methyl transferase

Homocysteine	Betaine-homocysteine methyltransferase Homocysteine methyltransferase Methionine synthase

Other	Phosphatidyl ethanolamine methyltransferase DNMT3B Histone methyltransferase Thymidylate synthase DNA methyltransferase Thiopurine methyltransferase

2.1.2: Hydroxymethyl-,
Formyl- and Related

Hydroxymethyltransferase	Serine hydroxymethyltransferase 3-methyl-2-oxobutanoate hydroxymethyltransferase

Formyltransferase	Phosphoribosylglycinamide formyltransferase Inosine monophosphate synthase

Other	Glutamate formimidoyltransferase Aminomethyltransferase

2.1.3: Carboxy-
and Carbamoyl

Carboxy	methylmalonyl-CoA carboxytransferase

Carbamoyl	Aspartate carbamoyltransferase Ornithine carbamoyltransferase Oxamate carbamoyltransferase Putrescine carbamoyltransferase 3-hydroxymethylcephem carbamoyltransferase Lysine carbamoyltransferase N-acetylornithine carbamoyltransferase

2.1.4: Amidine

Arginine:glycine amidinotransferase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases(list) EC2 Transferases(list) EC3 Hydrolases(list) EC4 Lyases(list) EC5 Isomerases(list) EC6 Ligases(list)

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