23S rRNA (adenine2503-C8)-methyltransferase

23S rRNA (adenine^2503-C^8)-methyltransferase
Identifiers
EC number	2.1.1.224
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

23S rRNA (adenine²⁵⁰³-C⁸)-methyltransferase (EC 2.1.1.224, Cfr (gene)) is an enzyme with systematic name S-adenosyl-L-methionine:23S rRNA (adenine²⁵⁰³-C⁸)-methyltransferase.^{[1][2][3][4][5][6]} This enzyme catalyses the following chemical reaction

2 S-adenosyl-L-methionine + adenine²⁵⁰³ in 23S rRNA $\rightleftharpoons$ S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine²⁵⁰³ in 23S rRNA

This enzyme is a member of the 'AdoMet radical' (radical SAM) family.

References

1. Giessing, A.M., Jensen, S.S., Rasmussen, A., Hansen, L.H., Gondela, A., Long, K., Vester, B. and Kirpekar, F. (2009). "Identification of 8-methyladenosine as the modification catalyzed by the radical SAM methyltransferase Cfr that confers antibiotic resistance in bacteria". RNA 15: 327–336. doi:10.1261/rna.1371409. PMC 2648713. PMID 19144912.
2. Kaminska, K.H., Purta, E., Hansen, L.H., Bujnicki, J.M., Vester, B. and Long, K.S. (2010). "Insights into the structure, function and evolution of the radical-SAM 23S rRNA methyltransferase Cfr that confers antibiotic resistance in bacteria". Nucleic Acids Res. 38: 1652–1663. doi:10.1093/nar/gkp1142. PMC 2836569. PMID 20007606.
3. Yan, F., LaMarre, J.M., Röhrich, R., Wiesner, J., Jomaa, H., Mankin, A.S., Fujimori, D.G. (2010). "RlmN and Cfr are radical SAM enzymes involved in methylation of ribosomal RNA". J. Am. Chem. Soc. 132: 3953–3964. doi:10.1021/ja910850y. PMC 2859901. PMID 20184321.
4. Yan, F. and Fujimori, D.G. (2011). "RNA methylation by Radical SAM enzymes RlmN and Cfr proceeds via methylene transfer and hydride shift". Proc. Natl. Acad. Sci. USA 108 (10): 3930–3934. doi:10.1073/pnas.1017781108. PMC 3054002. PMID 21368151.
5. Grove, T.L., Benner, J.S., Radle, M.I., Ahlum, J.H., Landgraf, B.J., Krebs, C. and Booker, S.J. (2011). "A radically different mechanism for S-adenosylmethionine-dependent methyltransferases". Science 332 (6029): 604–607. doi:10.1126/science.1200877. PMID 21415317.
6. Boal, A.K., Grove, T.L., McLaughlin, M.I., Yennawar, N.H., Booker, S.J. and Rosenzweig, A.C. (2011). "Structural basis for methyl transfer by a radical SAM enzyme". Science 332 (6033): 544–545. doi:10.1126/science.1205358. PMID 21527678.

External links

• 23S rRNA (adenine2503-C8)-methyltransferase at the US National Library of Medicine Medical Subject Headings (MeSH)