23S rRNA (adenosine1067-2'-O)-methyltransferase

23S rRNA (adenosine¹⁰⁶⁷-2'-O)-methyltransferase (EC 2.1.1.230, 23S rRNA A1067 2'-methyltransferase, thiostrepton-resistance methylase, nosiheptide-resistance methyltransferase) is an enzyme with systematic name S-adenosyl-L-methionine:23S rRNA (adenosine¹⁰⁶⁷-2'-O)-methyltransferase.^[1]^[2]^[3]^[4] This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + adenosine¹⁰⁶⁷ in 23S rRNA

\rightleftharpoons

S-adenosyl-L-homocysteine + 2'-O-methyladenosine¹⁰⁶⁷ in 23S rRNA

The methylase that is responsible for autoimmunity in the thiostrepton producer Streptomyces azureus.

References

↑ Bechthold, A. and Floss, H.G. (1994). "Overexpression of the thiostrepton-resistance gene from Streptomyces azureus in Escherichia coli and characterization of recognition sites of the 23S rRNA A¹⁰⁶⁷ 2′-methyltransferase in the guanosine triphosphatase center of 23S ribosomal RNA". Eur. J. Biochem. 224: 431–437. doi:10.1111/j.1432-1033.1994.00431.x. PMID 7925357.
↑ Thompson, J., Schmidt, F. and Cundliffe, E. (1982). "Site of action of a ribosomal RNA methylase conferring resistance to thiostrepton". J. Biol. Chem. 257: 7915–7917. PMID 6806287.
↑ Thompson, J. and Cundliffe, E. (1981). "Purification and properties of an RNA methylase produced by Streptomyces azureus and involved in resistance to thiostrepton". J. Gen. Microbiol. 124: 291–297. doi:10.1099/00221287-124-2-291.
↑ Yang, H., Wang, Z., Shen, Y., Wang, P., Jia, X., Zhao, L., Zhou, P., Gong, R., Li, Z., Yang, Y., Chen, D., Murchie, A.I. and Xu, Y. (2010). "Crystal structure of the nosiheptide-resistance methyltransferase of Streptomyces actuosus". Biochemistry 49: 6440–6450. doi:10.1021/bi1005915. PMID 20550164.

External links

23S rRNA (adenosine1067-2'-O)-methyltransferase at the US National Library of Medicine Medical Subject Headings (MeSH)

Transferase: one carbon transferases (EC 2.1)

2.1.1: Methyl-

N-	Histamine N-methyltransferase Phenylethanolamine N-methyltransferase Amine N-methyltransferase Phosphatidylethanolamine N-methyltransferase

O-	5-hydroxyindole-O-methyltransferase/Acetylserotonin O-methyltransferase Catechol-O-methyl transferase

Homocysteine	Betaine-homocysteine methyltransferase Homocysteine methyltransferase Methionine synthase

Other	Phosphatidyl ethanolamine methyltransferase DNMT3B Histone methyltransferase Thymidylate synthase DNA methyltransferase Thiopurine methyltransferase

2.1.2: Hydroxymethyl-,
Formyl- and Related

Hydroxymethyltransferase	Serine hydroxymethyltransferase 3-methyl-2-oxobutanoate hydroxymethyltransferase

Formyltransferase	Phosphoribosylglycinamide formyltransferase Inosine monophosphate synthase

Other	Glutamate formimidoyltransferase Aminomethyltransferase

2.1.3: Carboxy-
and Carbamoyl

Carboxy	methylmalonyl-CoA carboxytransferase

Carbamoyl	Aspartate carbamoyltransferase Ornithine carbamoyltransferase Oxamate carbamoyltransferase Putrescine carbamoyltransferase 3-hydroxymethylcephem carbamoyltransferase Lysine carbamoyltransferase N-acetylornithine carbamoyltransferase

2.1.4: Amidine

Arginine:glycine amidinotransferase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases(list) EC2 Transferases(list) EC3 Hydrolases(list) EC4 Lyases(list) EC5 Isomerases(list) EC6 Ligases(list)

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