23S rRNA (uracil1939-C5)-methyltransferase
23S rRNA (uracil1939-C5)-methyltransferase (EC 2.1.1.190, RumA, RNA uridine methyltransferase A, YgcA) is an enzyme with systematic name S-adenosyl-L-methionine:23S rRNA (uracil1939-C5)-methyltransferase.[1][2][3][4][5][6] This enzyme catalyses the following chemical reaction
- S-adenosyl-L-methionine + uracil1939 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil1939 in 23S rRNA
The enzyme specifically methylates uracil1939 at C5 in 23S rRNA.
References
- ↑ Agarwalla, S., Kealey, J.T., Santi, D.V. and Stroud, R.M. (2002). "Characterization of the 23 S ribosomal RNA m5U1939 methyltransferase from Escherichia coli". J. Biol. Chem. 277 (11): 8835–8840. doi:10.1074/jbc.M111825200. PMID 11779873.
- ↑ Lee, T.T., Agarwalla, S. and Stroud, R.M. (2004). "Crystal structure of RumA, an iron-sulfur cluster containing E. coli ribosomal RNA 5-methyluridine methyltransferase". Structure 12 (3): 397–407. doi:10.1016/j.str.2004.02.009. PMID 15016356.
- ↑ Madsen, C.T., Mengel-Jorgensen, J., Kirpekar, F. and Douthwaite, S. (2003). "Identifying the methyltransferases for m5U747 and m5U1939 in 23S rRNA using MALDI mass spectrometry". Nucleic Acids Res. 31: 4738–4746. doi:10.1093/nar/gkg657. PMID 12907714.
- ↑ Persaud, C., Lu, Y., Vila-Sanjurjo, A., Campbell, J.L., Finley, J. and O'Connor, M. (2010). "Mutagenesis of the modified bases, m5U1939 and Ψ2504, in Escherichia coli 23S rRNA". Biochem. Biophys. Res. Commun. 392 (2): 223–227. doi:10.1016/j.bbrc.2010.01.021. PMID 20067766.
- ↑ Agarwalla, S., Stroud, R.M. and Gaffney, B.J. (2004). "Redox reactions of the iron-sulfur cluster in a ribosomal RNA methyltransferase, RumA: optical and EPR studies". J. Biol. Chem. 279 (33): 34123–34129. doi:10.1074/jbc.M405702200. PMID 15181002.
- ↑ Lee, T.T., Agarwalla, S. and Stroud, R.M. (2005). "A unique RNA Fold in the RumA-RNA-cofactor ternary complex contributes to substrate selectivity and enzymatic function". Cell 120 (5): 599–611. doi:10.1016/j.cell.2004.12.037. PMID 15766524.
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