23S rRNA (uridine2479-2'-O)-methyltransferase

23S rRNA (uridine²⁴⁷⁹-2'-O)-methyltransferase (EC 2.1.1.208, AviRb) is an enzyme with systematic name S-adenosyl-L-methionine:23S rRNA (uridine²⁴⁷⁹-2'-O)-methyltransferase.^[1]^[2]^[3] This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + uridine²⁴⁷⁹ in 23S rRNA

\rightleftharpoons

S-adenosyl-L-homocysteine + 2'-O-methyluridine²⁴⁷⁹ in 23S rRNA

Streptomyces viridochromogenes produces the antibiotic avilamycin A which binds to the 50S ribosomal subunit to inhibit protein synthesis.

References

↑ Mosbacher, T.G., Bechthold, A. and Schulz, G.E. (2005). "Structure and function of the antibiotic resistance-mediating methyltransferase AviRb from Streptomyces viridochromogenes". J. Mol. Biol. 345: 535–545. doi:10.1016/j.jmb.2004.10.051. PMID 15581897.
↑ Treede, I., Jakobsen, L., Kirpekar, F., Vester, B., Weitnauer, G., Bechthold, A. and Douthwaite, S. (2003). "The avilamycin resistance determinants AviRa and AviRb methylate 23S rRNA at the guanosine 2535 base and the uridine 2479 ribose". Mol. Microbiol. 49: 309–318. doi:10.1046/j.1365-2958.2003.03558.x. PMID 12828631.
↑ Weitnauer, G., Gaisser, S., Trefzer, A., Stockert, S., Westrich, L., Quiros, L.M., Mendez, C., Salas, J.A. and Bechthold, A. (2001). "An ATP-binding cassette transporter and two rRNA methyltransferases are involved in resistance to avilamycin in the producer organism Streptomyces viridochromogenes Tu57". Antimicrob. Agents Chemother. 45: 690–695. doi:10.1128/aac.45.3.690-695.2001. PMID 11181344.

External links

23S rRNA (uridine2479-2'-O)-methyltransferase at the US National Library of Medicine Medical Subject Headings (MeSH)

Transferase: one carbon transferases (EC 2.1)

2.1.1: Methyl-

N-	Histamine N-methyltransferase Phenylethanolamine N-methyltransferase Amine N-methyltransferase Phosphatidylethanolamine N-methyltransferase

O-	5-hydroxyindole-O-methyltransferase/Acetylserotonin O-methyltransferase Catechol-O-methyl transferase

Homocysteine	Betaine-homocysteine methyltransferase Homocysteine methyltransferase Methionine synthase

Other	Phosphatidyl ethanolamine methyltransferase DNMT3B Histone methyltransferase Thymidylate synthase DNA methyltransferase Thiopurine methyltransferase

2.1.2: Hydroxymethyl-,
Formyl- and Related

Hydroxymethyltransferase	Serine hydroxymethyltransferase 3-methyl-2-oxobutanoate hydroxymethyltransferase

Formyltransferase	Phosphoribosylglycinamide formyltransferase Inosine monophosphate synthase

Other	Glutamate formimidoyltransferase Aminomethyltransferase

2.1.3: Carboxy-
and Carbamoyl

Carboxy	methylmalonyl-CoA carboxytransferase

Carbamoyl	Aspartate carbamoyltransferase Ornithine carbamoyltransferase Oxamate carbamoyltransferase Putrescine carbamoyltransferase 3-hydroxymethylcephem carbamoyltransferase Lysine carbamoyltransferase N-acetylornithine carbamoyltransferase

2.1.4: Amidine

Arginine:glycine amidinotransferase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases(list) EC2 Transferases(list) EC3 Hydrolases(list) EC4 Lyases(list) EC5 Isomerases(list) EC6 Ligases(list)

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