Trans-cinnamate 4-monooxygenase

trans-cinnamate 4-monooxygenase
Identifiers
EC number	1.14.13.11
CAS number	9077-75-2
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / EGO
Search PMC articles PubMed articles NCBI proteins

In enzymology, a trans-cinnamate 4-monooxygenase (EC 1.14.13.11) is an enzyme that catalyzes the chemical reaction

trans-cinnamate + NADPH + H⁺ + O₂ $\rightleftharpoons$ 4-hydroxycinnamate + NADP⁺ + H₂O

The 4 substrates of this enzyme are trans-cinnamate, NADPH, H⁺, and O₂, whereas its 3 products are 4-hydroxycinnamate, NADP⁺, and H₂O. This enzyme participates in phenylalanine metabolism and phenylpropanoid biosynthesis. It employs one cofactor, heme.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O₂ as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of one atom o oxygen into the other donor.

Nomenclature

The systematic name of this enzyme class is trans-cinnamate,NADPH:oxygen oxidoreductase (4-hydroxylating). Other names in common use include:

• cinnamic acid 4-hydroxylase,
• oxygenase, cinnamate 4-mono-,
• CA4H,
• cytochrome P450 cinnamate 4-hydroxylase,
• cinnamate 4-hydroxylase,
• cinnamate 4-monooxygenase,
• cinnamate hydroxylase,
• cinnamic 4-hydroxylase,
• cinnamic acid 4-monooxygenase,
• cinnamic acid p-hydroxylase,
• hydroxylase, cinnamate 4-,
• t-cinnamic acid hydroxylase,
• trans-cinnamate 4-hydroxylase, and
• trans-cinnamic acid 4-hydroxylase.

References

Further reading

• Potts JR, Weklych R, Conn EE, Rowell J (1974). "The 4-hydroxylation of cinnamic acid by sorghum microsomes and the requirement for cytochrome P-450". J. Biol. Chem. 249 (16): 5019–26. PMID 4153152.
• Russell DW, Conn EE (1967). "The cinnamic acid 4-hydroxylase of pea seedlings". Arch. Biochem. Biophys. 122 (1): 256–8. doi:10.1016/0003-9861(67)90150-6. PMID 4383827.
• Petersen M (May 2003). "Cinnamic acid 4-hydroxylase from cell cultures of the hornwort Anthoceros agrestis". Planta 217 (1): 96–101. doi:10.1007/s00425-002-0960-9. PMID 12721853.