9beta-pimara-7,15-diene oxidase

9beta-pimara-7,15-diene oxidase
Identifiers
EC number	1.14.13.144
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

9beta-pimara-7,15-diene oxidase (EC 1.14.13.144, CYP99A3) is an enzyme with systematic name 9beta-pimara-7,15-diene,NADPH:oxygen 19-oxidoreductase.^[1] This enzyme catalyses the following chemical reaction

9beta-pimara-7,15-diene + 3 O₂ + 3 NADPH + 3 H⁺ $\rightleftharpoons$ 9beta-pimara-7,15-dien-19-oate + 3 NADP⁺ + 4 H₂O (overall reaction)

(1a) 9beta-pimara-7,15-diene + O₂ + NADPH + H⁺ $\rightleftharpoons$ 9beta-pimara-7,15-dien-19-ol + NADP⁺ + H₂O

(1b) 9beta-pimara-7,15-dien-19-ol + O₂ + NADPH + H⁺ $\rightleftharpoons$ 9beta-pimara-7,15-dien-19-al + NADP⁺ + 2 H₂O

(1c) 9beta-pimara-7,15-dien-19-al + O₂ + NADPH + H⁺ $\rightleftharpoons$ 9beta-pimara-7,15-dien-19-oate + NADP⁺ + H₂O

9beta-pimara-7,15-diene oxidase requires cytochrome P450.

References

1. Wang, Q., Hillwig, M.L. and Peters, R.J. (2011). "CYP99A3: functional identification of a diterpene oxidase from the momilactone biosynthetic gene cluster in rice". Plant J. 65 (1): 87–95. doi:10.1111/j.1365-313X.2010.04408.x. PMID 21175892.

External links

• 9beta-pimara-7,15-diene oxidase at the US National Library of Medicine Medical Subject Headings (MeSH)