ADP-glyceromanno-heptose 6-epimerase

ADP-glyceromanno-heptose 6-epimerase
Identifiers
EC number	5.1.3.20
CAS number	85030-75-7
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / EGO
Search PMC articles PubMed articles NCBI proteins

In enzymology, an ADP-L-glycero-D-manno-heptose 6-epimerase (EC 5.1.3.20) is an enzyme that catalyzes the chemical reaction

ADP-D-glycero-D-manno-heptose $\rightleftharpoons$ ADP-L-glycero-D-manno-heptose

Hence, this enzyme has one substrate, ADP-D-glycero-D-manno-heptose, and one product, ADP-L-glycero-D-manno-heptose.

This enzyme belongs to the family of isomerases, specifically those racemases and epimerases acting on carbohydrates and derivatives. The systematic name of this enzyme class is ADP-L-glycero-D-manno-heptose 6-epimerase. This enzyme participates in lipopolysaccharide biosynthesis. It employs one cofactor, NADP+ in a direct oxidation mechanism.

Structural studies

As of 2007, only one structure has been solved for this class of enzymes, with the PDB accession code 1EQ2.

References

• Read JA, Ahmed RA, Morrison JP, Coleman WG Jr, Tanner ME (2004). "The Mechanism of the Reaction Catalyzed by ADP-β-L-glycero-D-manno-heptose 6-Epimerase". J. Am. Chem. Soc. 126: 8878–9. doi:10.1021/ja0485659. PMID 15264802.
• Ding L, Seto BL, Ahmed SA, Coleman WG Jr (1994). "Purification and properties of the Escherichia coli K-12 NAD-dependent nucleotide diphosphosugar epimerase, ADP-L-glycero-D-mannoheptose 6-epimerase". J. Biol. Chem. 269 (39): 24384–90. PMID 7929099.
• Raetz CR (1990). "Biochemistry of endotoxins". Annu. Rev. Biochem. 59: 129–70. doi:10.1146/annurev.bi.59.070190.001021. PMID 1695830.