Aminopyrimidine aminohydrolase

Aminopyrimidine aminohydrolase
Identifiers
EC number	3.5.99.2
CAS number	9024-80-0
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Aminopyrimidine aminohydrolase (EC 3.5.99.2, thiaminase, thiaminase II, tenA (gene)) is an enzyme with systematic name 4-amino-5-aminomethyl-2-methylpyrimidine aminohydrolase.^{[1][2][3][4][5][6][7]} This enzyme catalyses the following chemical reaction

(1) 4-amino-5-aminomethyl-2-methylpyrimidine + H₂O $\rightleftharpoons$ 4-amino-5-hydroxymethyl-2-methylpyrimidine + ammonia

(2) thiamine + H₂O $\rightleftharpoons$ 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole

This enzyme was previously known as thiaminase II.

References

1. Fujita, A., Nose, Y. and Kuratani, K. (1954). "Second type of bacterial thiaminase". J. Vitaminol. (Kyoto) 1 (1): 1–7. doi:10.5925/jnsv1954.1.1. PMID 13243520.
2. Ikehata, H. (1960). "Purification of thiaminase II". J. Gen. Appl. Microbiol. 6: 30–39. doi:10.2323/jgam.6.30.
3. Toms, A.V., Haas, A.L., Park, J.H., Begley, T.P. and Ealick, S.E. (2005). "Structural characterization of the regulatory proteins TenA and TenI from Bacillus subtilis and identification of TenA as a thiaminase II". Biochemistry 44: 2319–2329. doi:10.1021/bi0478648. PMID 15709744.
4. Benach, J., Edstrom, W.C., Lee, I., Das, K., Cooper, B., Xiao, R., Liu, J., Rost, B., Acton, T.B., Montelione, G.T. and Hunt, J.F. (2005). "The 2.35 Å structure of the TenA homolog from Pyrococcus furiosus supports an enzymatic function in thiamine metabolism". Acta Crystallogr. D Biol. Crystallogr. 61 (Pt 5): 589–598. doi:10.1107/S0907444905005147. PMID 15858269.
5. Jenkins, A.H., Schyns, G., Potot, S., Sun, G. and Begley, T.P. (2007). "A new thiamin salvage pathway". Nat. Chem. Biol. 3 (8): 492–497. doi:10.1038/nchembio.2007.13. PMID 17618314.
6. Jenkins, A.L., Zhang, Y., Ealick, S.E. and Begley, T.P. (2008). "Mutagenesis studies on TenA: a thiamin salvage enzyme from Bacillus subtilis". Bioorg. Chem. 36 (1): 29–32. doi:10.1016/j.bioorg.2007.10.005. PMID 18054064.
7. French, J.B., Begley, T.P. and Ealick, S.E. (2011). "Structure of trifunctional THI20 from yeast". Acta Crystallogr. D Biol. Crystallogr. 67 (Pt 9): 784–791. doi:10.1107/S0907444911024814. PMID 21904031.

External links

• Aminopyrimidine aminohydrolase at the US National Library of Medicine Medical Subject Headings (MeSH)