Amorpha-4,11-diene 12-monooxygenase

Amorpha-4,11-diene 12-monooxygenase
Identifiers
EC number	1.14.13.158
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Amorpha-4,11-diene 12-monooxygenase (EC 1.14.13.158, CYP71AV1) is an enzyme with systematic name amorpha-4,11-diene,NADPH:oxygen oxidoreductase (12-hydroxylating).^[1] This enzyme catalyses the following chemical reaction

amorpha-4,11-diene + 3 O₂ + 3 NADPH + 3 H⁺ $\rightleftharpoons$ artemisinate + 3 NADP⁺ + 4 H₂O (overall reaction)

(1a) amorpha-4,11-diene + O₂ + NADPH + H⁺ $\rightleftharpoons$ artemisinic alcohol + NADP⁺ + H₂O

(1b) artemisinic alcohol + O₂ + NADPH + H⁺ $\rightleftharpoons$ artemisinic aldehyde + NADP⁺ + 2 H₂O

(1c) artemisinic aldehyde + O₂ + NADPH + H⁺ $\rightleftharpoons$ artemisinate + NADP⁺ + H₂O

Amorpha-4,11-diene 12-monooxygenase is a heme-thiolate protein (P-450)

References

1. Teoh, K.H., Polichuk, D.R., Reed, D.W., Nowak, G. and Covello, P.S. (2006). "Artemisia annua L. (Asteraceae) trichome-specific cDNAs reveal CYP71AV1, a cytochrome P450 with a key role in the biosynthesis of the antimalarial sesquiterpene lactone artemisinin". FEBS Lett. 580 (5): 1411–1416. doi:10.1016/j.febslet.2006.01.065. PMID 16458889.

External links

• Amorpha-4,11-diene 12-monooxygenase at the US National Library of Medicine Medical Subject Headings (MeSH)