Auramine phenol stain

Auramine phenol stain is a stain used in clinical microbiology and histology to identify tuberculosis mycobacteria.

There are two types of auramine phenol stains, 1 and 2 to stain mycobacterium species and cryptosporidium respectively. Both are fluorescent stains. The bacteria or the parasites appear brilliant greenish yellow against dark background.^[1] Mycolic acids of the mycobacteria keep this stain when decolorising with the acid alcohol.The method is more rapid and sensitive than ZN technique.^[2]

Method

Smears are prepared just like that for ZN staining
Stain with Auramine-Phenol for 20 mins
Rinse with water
Decolourise in acid alcohol
Rinse with water
Counterstain with 0.1% potassium permanganate for 30 seconds
Rinse and air dry^[3]

References

↑ "Microbiology training log". Retrieved 19 November 2012.
↑ "Comparison of the value of two different sputum staining for diagnosis of acid-fast bacilli". Retrieved 19 November 2012.
↑ "Parasitology stains". Retrieved 19 November 2012.

Tuberculosis

Treatment/vaccines	ATC code J04 Isoniazid 4-Aminosalicylic acid Ethambutol Capreomycin Cycloserine Rifampicin Thioacetazone Streptomycin Bedaquiline RBCG30 Pyrazinamide MVA85A Rifater

History of tuberculosis	Manuel de Abreu Hermann Brehmer Albert Calmette Philip D'Arcy Hart Christopher Dye George M. Heath Max Gerson F. R. G. Heaf Robert Koch Marcos Espinal Friedrich Franz Friedmann Charles Mantoux Richard Morton Edward Livingston Trudeau Carl Rüedi Lucius Rüedi Mario Raviglione Madonna Swan

Conditions/ symptoms/signs	Caseous necrosis Ghon focus/Ghon's complex Giant multinucleated cell Pott disease Canga's bead symptom Prosector's wart Latent tuberculosis Paronychia Lupus vulgaris Tuberculous lymphadenitis Tuberculous meningitis Miliary tuberculosis

Mycobacterium species	Mycobacterium tuberculosis Mycobacterium africanum Mycobacterium bovis Mycobacterium bovis BCG Mycobacterium caprae

Type by resistance	Multi-drug-resistant tuberculosis Extensively drug-resistant tuberculosis Totally drug-resistant tuberculosis

Tuberculosis diagnosis	Ziehl–Neelsen stain Auramine phenol stain Culture on Löwenstein–Jensen medium and/or MGIT GeneXpert Chest photofluorography Tuberculin Heaf test Mantoux test Tine test Interferon gamma release assay QuantiFERON T-SPOT.TB Microscopic Observation Drug Susceptibility assay

Organizations	Adirondack Cottage Sanitarium Aeras Friends of the Global Fight Against AIDS, Tuberculosis, and Malaria Glen Lake Children's Camp Glen Lake Sanatorium Glenn Dale Hospital The Global Fund to Fight AIDS, Tuberculosis and Malaria Global Plan to Stop Tuberculosis Unitaid TB Alliance Seattle Biomed Stop TB Partnership Millennium Foundation Mycobacterium Tuberculosis Structural Genomics Consortium National Jewish Health Phipps Institute for the Study, Treatment and Prevention of Tuberculosis Campaign for Access to Essential Medicines Cure Cottages of Saranac Lake International Congress on Tuberculosis

Other/ungrouped	2007 tuberculosis scare 72F fusion protein vaccine Tuberculosis in popular culture Baumgarten-Tangl law CFP-10 Tuberculosis in China ESAT-6 Iowa Cow War List of tuberculosis cases Plombage Preventorium Sanatorium Sunshine Way Tuberculosis classification Tuberculosis radiology Tygerberg score World Tuberculosis Day

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