Benzoate 4-monooxygenase

benzoate 4-monooxygenase
Identifiers
EC number	1.14.13.12
CAS number	39391-25-8
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / EGO
Search PMC articles PubMed articles NCBI proteins

In enzymology, a benzoate 4-monooxygenase (EC 1.14.13.12) is an enzyme that catalyzes the chemical reaction

benzoate + NADPH + H⁺ + O₂ $\rightleftharpoons$ 4-hydroxybenzoate + NADP⁺ + H₂O

The 4 substrates of this enzyme are benzoate, NADPH, H⁺, and O₂, whereas its 3 products are 4-hydroxybenzoate, NADP⁺, and H₂O.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of one atom o oxygen into the other donor. The systematic name of this enzyme class is benzoate,NADPH:oxygen oxidoreductase (4-hydroxylating). Other names in common use include benzoic acid 4-hydroxylase, benzoate 4-hydroxylase, benzoic 4-hydroxylase, benzoate-p-hydroxylase, and p-hydroxybenzoate hydroxylase. This enzyme participates in benzoate degradation via hydroxylation and benzoate degradation via coa ligation. It has 3 cofactors: iron, Tetrahydrobiopterin, and Tetrahydropteridine.

References

• Reddy CC, Vaidyanathan CS (1975). "Purification, properties and induction of a specific benzoate-4-hydroxylase from Aspergillus niger (UBC 814)". Biochim. Biophys. Acta 384 (1): 46–57. doi:10.1016/0005-2744(75)90094-7. PMID 236777.