CASC5

Cancer susceptibility candidate 5
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 3SI5​, 4A1G​, 4NF9​, 4NFA​
Identifiers
Symbols	CASC5 ; AF15Q14; CT29; D40; KNL1; PPP1R55; Spc7; hKNL-1; hSpc105
External IDs	OMIM: 609173 MGI: 1923714 HomoloGene: 44890 GeneCards: CASC5 Gene
Gene ontology Molecular function • protein binding Cellular component • condensed chromosome kinetochore • acrosomal vesicle • nucleus • nucleoplasm • cytoplasm • cytosol • extracellular exosome Biological process • mitotic cell cycle • acrosome assembly • nucleosome assembly • mitotic nuclear division • small GTPase mediated signal transduction • attachment of spindle microtubules to kinetochore • negative regulation of phosphatase activity • CENP-A containing nucleosome assembly • protein localization to kinetochore • cell division • spindle assembly checkpoint Sources: Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	57082	76464
Ensembl	ENSG00000137812	ENSMUSG00000027326
UniProt	Q8NG31	Q66JQ7
RefSeq (mRNA)	NM_144508	NM_029617
RefSeq (protein)	NP_653091	NP_083893
Location (UCSC)	Chr 15: 40.59 – 40.66 Mb	Chr 2: 119.05 – 119.11 Mb
PubMed search

CASC5 is a protein that is encoded by the CASC5 gene in humans.^[1][2][3][4]

Function

CASC5 is part of the kinetochore. It is involved in microtubule attachment to chromosome centromeres and in the activation of the spindle checkpoint during mitosis. The CASC5 gene is upregulated in the areas of cell proliferation surrounding the ventricles during fetal brain development.^[5]

Interactions

CASC5 has been shown to interact with MIS12,^[6][7] BUB1, BUBR1 and ZWINT-1.^[5]

Polymorphisms

Homozygous polymorphisms in the CASC5 gene have been seen in patients with autosomal recessive primary microcephaly (MCPH). The mutation resulted in the skipping of exon 18 transcription, causing a frameshift and the production of a truncated protein. This truncation inhibits the binding ability of MIS12.^[5]

References

1. Hayette S, Tigaud I, Vanier A, Martel S, Corbo L, Charrin C, Beillard E, Deleage G, Magaud JP, Rimokh R (Oct 2000). "AF15q14, a novel partner gene fused to the MLL gene in an acute myeloid leukaemia with a t(11;15)(q23;q14)". Oncogene 19 (38): 4446–50. doi:10.1038/sj.onc.1203789. PMID 10980622.
2. Wei G, Takimoto M, Yoshida I, Mao PZ, Koya RC, Miura T, Kuzumaki N (Jun 2000). "Chromosomal assignment of a novel human gene D40". Nucleic Acids Symp Ser (42): 71–2. PMID 10780384.
3. Cheeseman IM, Hori T, Fukagawa T, Desai A (Feb 2008). "KNL1 and the CENP-H/I/K complex coordinately direct kinetochore assembly in vertebrates". Mol Biol Cell 19 (2): 587–94. doi:10.1091/mbc.E07-10-1051. PMC 2230600. PMID 18045986.
4. "Entrez Gene: CASC5 cancer susceptibility candidate 5".
5. Genin A; et al. (Dec 2012). "Kinetochore KMN network gene CASC5 mutated in primary microcephaly.". Hum Mol Genet. 21 (24): 5306–17. doi:10.1093/hmg/dds386.
6. Cheeseman, Iain M; Niessen Sherry; Anderson Scott; Hyndman Francie; Yates John R; Oegema Karen; Desai Arshad (Sep 2004). "A conserved protein network controls assembly of the outer kinetochore and its ability to sustain tension". Genes Dev. (United States) 18 (18): 2255–68. doi:10.1101/gad.1234104. ISSN 0890-9369. PMC 517519. PMID 15371340.
7. Obuse, Chikashi; Iwasaki Osamu; Kiyomitsu Tomomi; Goshima Gohta; Toyoda Yusuke; Yanagida Mitsuhiro (Nov 2004). "A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1". Nat. Cell Biol. (England) 6 (11): 1135–41. doi:10.1038/ncb1187. ISSN 1465-7392. PMID 15502821.

Further reading

• Ray ME, Su YA, Meltzer PS, Trent JM (1996). "Isolation and characterization of genes associated with chromosome-6 mediated tumor suppression in human malignant melanoma.". Oncogene 12 (12): 2527–33. PMID 8700511.
• Nagase T, Kikuno R, Nakayama M; et al. (2001). "Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.". DNA Res. 7 (4): 273–81. doi:10.1093/dnares/7.4.271. PMID 10997877.
• Takimoto M, Wei G, Dosaka-Akita H; et al. (2002). "Frequent expression of new cancer/testis gene D40/AF15q14 in lung cancers of smokers.". Br. J. Cancer 86 (11): 1757–62. doi:10.1038/sj.bjc.6600328. PMC 2375411. PMID 12087463.
• Strausberg RL, Feingold EA, Grouse LH; et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Chinwalla V, Chien A, Odero M; et al. (2003). "A t(11;15) fuses MLL to two different genes, AF15q14 and a novel gene MPFYVE on chromosome 15.". Oncogene 22 (9): 1400–10. doi:10.1038/sj.onc.1206273. PMID 12618766.
• Kuefer MU, Chinwalla V, Zeleznik-Le NJ; et al. (2003). "Characterization of the MLL partner gene AF15q14 involved in t(11;15)(q23;q14).". Oncogene 22 (9): 1418–24. doi:10.1038/sj.onc.1206272. PMID 12618768.
• Cheeseman IM, Niessen S, Anderson S; et al. (2004). "A conserved protein network controls assembly of the outer kinetochore and its ability to sustain tension.". Genes Dev. 18 (18): 2255–68. doi:10.1101/gad.1234104. PMC 517519. PMID 15371340.
• Obuse C, Iwasaki O, Kiyomitsu T; et al. (2004). "A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1.". Nat. Cell Biol. 6 (11): 1135–41. doi:10.1038/ncb1187. PMID 15502821.
• Sasao T, Itoh N, Takano H; et al. (2005). "The protein encoded by cancer/testis gene D40/AF15q14 is localized in spermatocytes, acrosomes of spermatids and ejaculated spermatozoa.". Reproduction 128 (6): 709–16. doi:10.1530/rep.1.00312. PMID 15579588.
• Nousiainen M, Silljé HH, Sauer G; et al. (2006). "Phosphoproteome analysis of the human mitotic spindle.". Proc. Natl. Acad. Sci. U.S.A. 103 (14): 5391–6. doi:10.1073/pnas.0507066103. PMC 1459365. PMID 16565220.
• Olsen JV, Blagoev B, Gnad F; et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.". Cell 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983.