CDC45-related protein

Cell division cycle 45
Identifiers
Symbols	CDC45 ; CDC45L; CDC45L2; PORC-PI-1
External IDs	OMIM: 603465 MGI: 1338073 HomoloGene: 2616 ChEMBL: 3040 GeneCards: CDC45 Gene
Gene ontology Molecular function • chromatin binding • DNA replication origin binding • single-stranded DNA binding • protein binding • 3'-5' DNA helicase activity Cellular component • nucleus • nucleoplasm • nuclear pre-replicative complex • cytoplasm • centrosome • DNA replication preinitiation complex • replication fork protection complex Biological process • DNA replication checkpoint • G1/S transition of mitotic cell cycle • regulation of transcription involved in G1/S transition of mitotic cell cycle • mitotic cell cycle • double-strand break repair via break-induced replication • DNA replication • pre-replicative complex assembly involved in nuclear cell cycle DNA replication • DNA replication initiation • DNA strand elongation involved in DNA replication • regulation of chromatin silencing at telomere • DNA duplex unwinding • positive regulation of G1/S transition of mitotic cell cycle • mitotic DNA replication preinitiation complex assembly Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	8318	12544
Ensembl	ENSG00000093009	ENSMUSG00000000028
UniProt	O75419	Q9Z1X9
RefSeq (mRNA)	NM_001178010	NM_001161623
RefSeq (protein)	NP_001171481	NP_001155095
Location (UCSC)	Chr 22: 19.48 – 19.52 Mb	Chr 16: 18.78 – 18.81 Mb
PubMed search

CDC45 is a protein that in humans is encoded by the CDC45L gene.^[1][2][3][4]

Function

The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple polyadenlyation sites of this gene are reported.^[4]

Interactions

CDC45-related protein has been shown to interact with:

• MCM3,^[5]
• MCM6,^[5]
• MCM7,^[5][6]
• ORC1L,^[5] and
• ORC6L.^[5]

References

1. Saha P, Thome KC, Yamaguchi R, Hou Z, Weremowicz S, Dutta A (Aug 1998). "The human homolog of Saccharomyces cerevisiae CDC45". J. Biol. Chem. 273 (29): 18205–9. doi:10.1074/jbc.273.29.18205. PMID 9660782.
2. McKie JM, Wadey RB, Sutherland HF, Taylor CL, Scambler PJ (Sep 1998). "Direct selection of conserved cDNAs from the DiGeorge critical region: isolation of a novel CDC45-like gene". Genome Res. 8 (8): 834–41. doi:10.1101/gr.8.8.834. PMC 310757. PMID 9724329.
3. Pollok S, Bauerschmidt C, Sänger J, Nasheuer HP, Grosse F (Jul 2007). "Human Cdc45 is a proliferation-associated antigen". FEBS J. 274 (14): 3669–84. doi:10.1111/j.1742-4658.2007.05900.x. PMID 17608804.
4. "Entrez Gene: CDC45L CDC45 cell division cycle 45-like (S. cerevisiae)".
5. Kneissl M, Pütter V, Szalay AA, Grummt F (Mar 2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". J. Mol. Biol. 327 (1): 111–28. doi:10.1016/s0022-2836(03)00079-2. PMID 12614612.
6. Kukimoto I, Igaki H, Kanda T (Nov 1999). "Human CDC45 protein binds to minichromosome maintenance 7 protein and the p70 subunit of DNA polymerase alpha". Eur. J. Biochem. 265 (3): 936–43. doi:10.1046/j.1432-1327.1999.00791.x. PMID 10518787.

Further reading

• Leatherwood J (1998). "Emerging mechanisms of eukaryotic DNA replication initiation". Curr. Opin. Cell Biol. 10 (6): 742–8. doi:10.1016/S0955-0674(98)80117-8. PMID 9914182.
• Mimura S, Takisawa H (1998). "Xenopus Cdc45-dependent loading of DNA polymerase alpha onto chromatin under the control of S-phase Cdk". EMBO J. 17 (19): 5699–707. doi:10.1093/emboj/17.19.5699. PMC 1170898. PMID 9755170.
• Shaikh TH, Gottlieb S, Sellinger B, Chen F, Roe BA, Oakey RJ, Emanuel BS, Budarf ML (1999). "Characterization of CDC45L: a gene in the 22q11.2 deletion region expressed during murine and human development". Mamm. Genome 10 (3): 322–6. doi:10.1007/s003359900996. PMID 10051334.
• Kukimoto I, Igaki H, Kanda T (1999). "Human CDC45 protein binds to minichromosome maintenance 7 protein and the p70 subunit of DNA polymerase alpha". Eur. J. Biochem. 265 (3): 936–43. doi:10.1046/j.1432-1327.1999.00791.x. PMID 10518787.
• Kneissl M, Pütter V, Szalay AA, Grummt F (2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". J. Mol. Biol. 327 (1): 111–28. doi:10.1016/S0022-2836(03)00079-2. PMID 12614612.
• Clark HF, Gurney AL, Abaya E, Baker K, Baldwin D, Brush J, Chen J, Chow B, Chui C, Crowley C, Currell B, Deuel B, Dowd P, Eaton D, Foster J, Grimaldi C, Gu Q, Hass PE, Heldens S, Huang A, Kim HS, Klimowski L, Jin Y, Johnson S, Lee J, Lewis L, Liao D, Mark M, Robbie E, Sanchez C, Schoenfeld J, Seshagiri S, Simmons L, Singh J, Smith V, Stinson J, Vagts A, Vandlen R, Watanabe C, Wieand D, Woods K, Xie MH, Yansura D, Yi S, Yu G, Yuan J, Zhang M, Zhang Z, Goddard A, Wood WI, Godowski P, Gray A (2003). "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment". Genome Res. 13 (10): 2265–70. doi:10.1101/gr.1293003. PMC 403697. PMID 12975309.
• Ramachandran N, Hainsworth E, Bhullar B, Eisenstein S, Rosen B, Lau AY, Walter JC, LaBaer J (2004). "Self-assembling protein microarrays". Science 305 (5680): 86–90. doi:10.1126/science.1097639. PMID 15232106.
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
• Liu P, Barkley LR, Day T, Bi X, Slater DM, Alexandrow MG, Nasheuer HP, Vaziri C (2006). "The Chk1-mediated S-phase checkpoint targets initiation factor Cdc45 via a Cdc25A/Cdk2-independent mechanism". J. Biol. Chem. 281 (41): 30631–44. doi:10.1074/jbc.M602982200. PMID 16912045.
• Masai H, Taniyama C, Ogino K, Matsui E, Kakusho N, Matsumoto S, Kim JM, Ishii A, Tanaka T, Kobayashi T, Tamai K, Ohtani K, Arai K (2006). "Phosphorylation of MCM4 by Cdc7 kinase facilitates its interaction with Cdc45 on the chromatin". J. Biol. Chem. 281 (51): 39249–61. doi:10.1074/jbc.M608935200. PMID 17046832.
• Boskovic J, Coloma J, Aparicio T, Zhou M, Robinson CV, Méndez J, Montoya G (2007). "Molecular architecture of the human GINS complex". EMBO Rep. 8 (7): 678–84. doi:10.1038/sj.embor.7401002. PMC 1905900. PMID 17557111.
• Bauerschmidt C, Pollok S, Kremmer E, Nasheuer HP, Grosse F (2007). "Interactions of human Cdc45 with the Mcm2-7 complex, the GINS complex, and DNA polymerases delta and epsilon during S phase". Genes Cells 12 (6): 745–58. doi:10.1111/j.1365-2443.2007.01090.x. PMID 17573775.
• Pollok S, Grosse F (2007). "Cdc45 degradation during differentiation and apoptosis". Biochem. Biophys. Res. Commun. 362 (4): 910–5. doi:10.1016/j.bbrc.2007.08.069. PMID 17767920.