MCM3

Minichromosome maintenance complex component 3
Identifiers
Symbols MCM3 ; HCC5; P1-MCM3; P1.h; RLFB
External IDs OMIM: 602693 MGI: 101845 HomoloGene: 1791 GeneCards: MCM3 Gene
EC number 3.6.4.12
RNA expression pattern
More reference expression data
Orthologs
Species Human Mouse
Entrez 4172 17215
Ensembl ENSG00000112118 ENSMUSG00000041859
UniProt P25205 P25206
RefSeq (mRNA) NM_001270472 NM_008563
RefSeq (protein) NP_001257401 NP_032589
Location (UCSC) Chr 6:
52.26 – 52.28 Mb
Chr 1:
20.8 – 20.82 Mb
PubMed search

DNA replication licensing factor MCM3 is a protein that in humans is encoded by the MCM3 gene.[1]

Function

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.[2]

Interactions

MCM3 has been shown to interact with:

See also

References

  1. Thömmes P, Fett R, Schray B, Burkhart R, Barnes M, Kennedy C, Brown NC, Knippers R (April 1992). "Properties of the nuclear P1 protein, a mammalian homologue of the yeast Mcm3 replication protein". Nucleic Acids Res 20 (5): 1069–74. doi:10.1093/nar/20.5.1069. PMC 312092. PMID 1549468.
  2. "Entrez Gene: MCM3 MCM3 minichromosome maintenance deficient 3 (S. cerevisiae)".
  3. 1 2 3 4 5 6 7 Kneissl M, Pütter V, Szalay AA, Grummt F (March 2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". J. Mol. Biol. 327 (1): 111–28. doi:10.1016/s0022-2836(03)00079-2. PMID 12614612.
  4. 1 2 Fujita M, Yamada C, Goto H, Yokoyama N, Kuzushima K, Inagaki M, Tsurumi T (September 1999). "Cell cycle regulation of human CDC6 protein. Intracellular localization, interaction with the human mcm complex, and CDC2 kinase-mediated hyperphosphorylation". J. Biol. Chem. 274 (36): 25927–32. doi:10.1074/jbc.274.36.25927. PMID 10464337.
  5. Méndez J, Stillman B (November 2000). "Chromatin association of human origin recognition complex, cdc6, and minichromosome maintenance proteins during the cell cycle: assembly of prereplication complexes in late mitosis". Mol. Cell. Biol. 20 (22): 8602–12. doi:10.1128/mcb.20.22.8602-8612.2000. PMC 102165. PMID 11046155.
  6. Takei Y, Assenberg M, Tsujimoto G, Laskey R (November 2002). "The MCM3 acetylase MCM3AP inhibits initiation, but not elongation, of DNA replication via interaction with MCM3". J. Biol. Chem. 277 (45): 43121–5. doi:10.1074/jbc.C200442200. PMID 12226073.
  7. Kuwahara K, Yoshida M, Kondo E, Sakata A, Watanabe Y, Abe E, Kouno Y, Tomiyasu S, Fujimura S, Tokuhisa T, Kimura H, Ezaki T, Sakaguchi N (April 2000). "A novel nuclear phosphoprotein, GANP, is up-regulated in centrocytes of the germinal center and associated with MCM3, a protein essential for DNA replication". Blood 95 (7): 2321–8. PMID 10733502.
  8. Takei Y, Tsujimoto G (August 1998). "Identification of a novel MCM3-associated protein that facilitates MCM3 nuclear localization". J. Biol. Chem. 273 (35): 22177–80. doi:10.1074/jbc.273.35.22177. PMID 9712829.
  9. DaFonseca CJ, Shu F, Zhang JJ (March 2001). "Identification of two residues in MCM5 critical for the assembly of MCM complexes and Stat1-mediated transcription activation in response to IFN-gamma". Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3034–9. doi:10.1073/pnas.061487598. PMC 30602. PMID 11248027.
  10. Fujita M, Kiyono T, Hayashi Y, Ishibashi M (April 1997). "In vivo interaction of human MCM heterohexameric complexes with chromatin. Possible involvement of ATP". J. Biol. Chem. 272 (16): 10928–35. doi:10.1074/jbc.272.16.10928. PMID 9099751.
  11. Fujita M, Ishimi Y, Nakamura H, Kiyono T, Tsurumi T (March 2002). "Nuclear organization of DNA replication initiation proteins in mammalian cells". J. Biol. Chem. 277 (12): 10354–61. doi:10.1074/jbc.M111398200. PMID 11779870.

Further reading

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