MCM3AP

Minichromosome maintenance complex component 3 associated protein
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 4DHX​
Identifiers
Symbols	MCM3AP ; GANP; MAP80; SAC3
External IDs	OMIM: 603294 MGI: 1930089 HomoloGene: 2902 GeneCards: MCM3AP Gene
Gene ontology Molecular function • DNA binding • transferase activity, transferring acyl groups Cellular component • nucleus • nuclear pore • nucleoplasm • cytoplasm • cytosol • nuclear membrane Biological process • immune system process • DNA replication • protein import into nucleus • mRNA transport Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	8888	54387
Ensembl	ENSG00000160294	ENSMUSG00000001150
UniProt	O60318	Q9WUU9
RefSeq (mRNA)	NM_003906	NM_019434
RefSeq (protein)	NP_003897	NP_062307
Location (UCSC)	Chr 21: 46.24 – 46.29 Mb	Chr 10: 76.47 – 76.52 Mb
PubMed search

80 kDa MCM3-associated protein is a protein that in humans is encoded by the MCM3AP gene.^[1][2]

Function

The minichromosome maintenance protein 3 (MCM3) is one of the MCM proteins essential for the initiation of DNA replication. The protein encoded by this gene is an MCM3 binding protein. It was reported to have phosphorylation-dependent DNA-primase activity, which was up-regulated in antigen immunization induced germinal center. This protein was demonstrated to be an acetyltransferase that acetylates MCM3 and plays a role in DNA replication. The mutagenesis of a nuclear localization signal of MCM3 affects the binding of this protein with MCM3, suggesting that this protein may also facilitate MCM3 nuclear localization.^[2]

Interactions

MCM3AP has been shown to interact with:

• MCM3^[1][3][4] and
• TRIB3.^[5]

References

1. Takei Y, Tsujimoto G (September 1998). "Identification of a novel MCM3-associated protein that facilitates MCM3 nuclear localization". J. Biol. Chem. 273 (35): 22177–80. doi:10.1074/jbc.273.35.22177. PMID 9712829.
2. "Entrez Gene: MCM3AP MCM3 minichromosome maintenance deficient 3 (S. cerevisiae) associated protein".
3. Takei Y, Assenberg M, Tsujimoto G, Laskey R (November 2002). "The MCM3 acetylase MCM3AP inhibits initiation, but not elongation, of DNA replication via interaction with MCM3". J. Biol. Chem. 277 (45): 43121–5. doi:10.1074/jbc.C200442200. PMID 12226073.
4. Kuwahara K, Yoshida M, Kondo E, Sakata A, Watanabe Y, Abe E, Kouno Y, Tomiyasu S, Fujimura S, Tokuhisa T, Kimura H, Ezaki T, Sakaguchi N (April 2000). "A novel nuclear phosphoprotein, GANP, is up-regulated in centrocytes of the germinal center and associated with MCM3, a protein essential for DNA replication". Blood 95 (7): 2321–8. PMID 10733502.
5. Zhou Y, Li L, Liu Q, Xing G, Kuai X, Sun J, Yin X, Wang J, Zhang L, He F (May 2008). "E3 ubiquitin ligase SIAH1 mediates ubiquitination and degradation of TRB3". Cell. Signal. 20 (5): 942–8. doi:10.1016/j.cellsig.2008.01.010. PMID 18276110.

Further reading

• Nagase T, Ishikawa K, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (1998). "Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Res. 5 (1): 31–9. doi:10.1093/dnares/5.1.31. PMID 9628581.
• Kuwahara K, Yoshida M, Kondo E, Sakata A, Watanabe Y, Abe E, Kouno Y, Tomiyasu S, Fujimura S, Tokuhisa T, Kimura H, Ezaki T, Sakaguchi N (2000). "A novel nuclear phosphoprotein, GANP, is up-regulated in centrocytes of the germinal center and associated with MCM3, a protein essential for DNA replication". Blood 95 (7): 2321–8. PMID 10733502.
• Abe E, Kuwahara K, Yoshida M, Suzuki M, Terasaki H, Matsuo Y, Takahashi EI, Sakaguchi N (2000). "Structure, expression, and chromosomal localization of the human gene encoding a germinal center-associated nuclear protein (GANP) that associates with MCM3 involved in the initiation of DNA replication". Gene 255 (2): 219–27. doi:10.1016/S0378-1119(00)00336-X. PMID 11024281.
• Takei Y, Swietlik M, Tanoue A, Tsujimoto G, Kouzarides T, Laskey R (2001). "MCM3AP, a novel acetyltransferase that acetylates replication protein MCM3". EMBO Rep. 2 (2): 119–23. doi:10.1093/embo-reports/kve026. PMC 1083822. PMID 11258703.
• Kuwahara K, Tomiyasu S, Fujimura S, Nomura K, Xing Y, Nishiyama N, Ogawa M, Imajoh-Ohmi S, Izuta S, Sakaguchi N (2001). "Germinal center-associated nuclear protein (GANP) has a phosphorylation-dependent DNA-primase activity that is up-regulated in germinal center regions". Proc. Natl. Acad. Sci. U.S.A. 98 (18): 10279–83. doi:10.1073/pnas.181335698. PMC 56952. PMID 11526238.
• EL-Gazzar MA, Maeda K, Nomiyama H, Nakao M, Kuwahara K, Sakaguchi N (2001). "PU.1 is involved in the regulation of B lineage-associated and developmental stage-dependent expression of the germinal center-associated DNA primase GANP". J. Biol. Chem. 276 (51): 48000–8. doi:10.1074/jbc.+M106696200. PMID 11641399.
• Kono Y, Maeda K, Kuwahara K, Yamamoto H, Miyamoto E, Yonezawa K, Takagi K, Sakaguchi N (2002). "MCM3-binding GANP DNA-primase is associated with a novel phosphatase component G5PR". Genes Cells 7 (8): 821–34. doi:10.1046/j.1365-2443.2002.00562.x. PMID 12167160.
• Takei Y, Assenberg M, Tsujimoto G, Laskey R (2002). "The MCM3 acetylase MCM3AP inhibits initiation, but not elongation, of DNA replication via interaction with MCM3". J. Biol. Chem. 277 (45): 43121–5. doi:10.1074/jbc.C200442200. PMID 12226073.
• Nakayama M, Kikuno R, Ohara O (2002). "Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs". Genome Res. 12 (11): 1773–84. doi:10.1101/gr.406902. PMC 187542. PMID 12421765.
• Sakaguchi N, Fujimura S, Kuwahara K (2002). "Involvement of GANP in B cell activation in T cell-dependent antigen response". Dev. Immunol. 9 (3): 169–72. doi:10.1080/1044667031000137647. PMC 2276099. PMID 12885157.
• Colland F, Jacq X, Trouplin V, Mougin C, Groizeleau C, Hamburger A, Meil A, Wojcik J, Legrain P, Gauthier JM (2004). "Functional proteomics mapping of a human signaling pathway". Genome Res. 14 (7): 1324–32. doi:10.1101/gr.2334104. PMC 442148. PMID 15231748.
• Hu YH, Warnatz HJ, Vanhecke D, Wagner F, Fiebitz A, Thamm S, Kahlem P, Lehrach H, Yaspo ML, Janitz M (2006). "Cell array-based intracellular localization screening reveals novel functional features of human chromosome 21 proteins". BMC Genomics 7: 155. doi:10.1186/1471-2164-7-155. PMC 1526728. PMID 16780588.