CHAF1B

Chromatin assembly factor 1, subunit B (p60)
Identifiers
Symbols	CHAF1B ; CAF-1; CAF-IP60; CAF1; CAF1A; CAF1P60; MPHOSPH7; MPP7
External IDs	OMIM: 601245 MGI: 1314881 HomoloGene: 48346 GeneCards: CHAF1B Gene
Gene ontology Molecular function • chromatin binding • histone binding • unfolded protein binding Cellular component • nuclear chromatin • nucleus • nucleoplasm • cytoplasm • cytosol • CAF-1 complex • protein complex Biological process • positive regulation of defense response to virus by host • DNA replication • DNA repair • DNA replication-dependent nucleosome assembly • transcription, DNA-templated • regulation of transcription, DNA-templated • protein complex assembly • cell cycle • chromatin assembly • activation of mitophagy in response to mitochondrial depolarization Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	8208	110749
Ensembl	ENSG00000159259	ENSMUSG00000022945
UniProt	Q13112	Q9D0N7
RefSeq (mRNA)	NM_005441	NM_028083
RefSeq (protein)	NP_005432	NP_082359
Location (UCSC)	Chr 21: 36.39 – 36.42 Mb	Chr 16: 93.88 – 93.91 Mb
PubMed search

Chromatin assembly factor 1 subunit B is a protein that in humans is encoded by the CHAF1B gene.^[1][2][3]

Function

Chromatin assembly factor I (CAF-1) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair.^[3]

Interactions

CHAF1B has been shown to interact with:

• ASF1A,^[4]
• ASF1B,^[4][5] and
• BAZ1B.^[6]

References

1. Kaufman PD, Kobayashi R, Kessler N, Stillman B (Jun 1995). "The p150 and p60 subunits of chromatin assembly factor I: a molecular link between newly synthesized histones and DNA replication". Cell 81 (7): 1105–14. doi:10.1016/S0092-8674(05)80015-7. PMID 7600578.
2. Katsanis N, Fisher EM (Oct 1996). "The gene encoding the p60 subunit of chromatin assembly factor I (CAF1P60) maps to human chromosome 21q22.2, a region associated with some of the major features of Down syndrome". Human Genetics 98 (4): 497–9. doi:10.1007/s004390050246. PMID 8792829.
3. "Entrez Gene: CHAF1B chromatin assembly factor 1, subunit B (p60)".
4. Mello JA, Silljé HH, Roche DM, Kirschner DB, Nigg EA, Almouzni G (Apr 2002). "Human Asf1 and CAF-1 interact and synergize in a repair-coupled nucleosome assembly pathway". EMBO Reports 3 (4): 329–34. doi:10.1093/embo-reports/kvf068. PMC 1084056. PMID 11897662.
5. Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology 3: 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.
6. Kitagawa H, Fujiki R, Yoshimura K, Mezaki Y, Uematsu Y, Matsui D, Ogawa S, Unno K, Okubo M, Tokita A, Nakagawa T, Ito T, Ishimi Y, Nagasawa H, Matsumoto T, Yanagisawa J, Kato S (Jun 2003). "The chromatin-remodeling complex WINAC targets a nuclear receptor to promoters and is impaired in Williams syndrome". Cell 113 (7): 905–17. doi:10.1016/s0092-8674(03)00436-7. PMID 12837248.

Further reading

• Ridgway P, Almouzni G (Aug 2000). "CAF-1 and the inheritance of chromatin states: at the crossroads of DNA replication and repair". Journal of Cell Science 113 (15): 2647–58. PMID 10893180.
• Blouin JL, Duriaux-Sail G, Chen H, Gos A, Morris MA, Rossier C, Antonarakis SE (Apr 1996). "Mapping of the gene for the p60 subunit of the human chromatin assembly factor (CAF1A) to the Down syndrome region of chromosome 21". Genomics 33 (2): 309–12. doi:10.1006/geno.1996.0199. PMID 8660983.
• Verreault A, Kaufman PD, Kobayashi R, Stillman B (Oct 1996). "Nucleosome assembly by a complex of CAF-1 and acetylated histones H3/H4". Cell 87 (1): 95–104. doi:10.1016/S0092-8674(00)81326-4. PMID 8858152.
• Matsumoto-Taniura N, Pirollet F, Monroe R, Gerace L, Westendorf JM (Sep 1996). "Identification of novel M phase phosphoproteins by expression cloning". Molecular Biology of the Cell 7 (9): 1455–69. doi:10.1091/mbc.7.9.1455. PMC 275994. PMID 8885239.
• Marheineke K, Krude T (Jun 1998). "Nucleosome assembly activity and intracellular localization of human CAF-1 changes during the cell division cycle". The Journal of Biological Chemistry 273 (24): 15279–86. doi:10.1074/jbc.273.24.15279. PMID 9614144.
• Martini E, Roche DM, Marheineke K, Verreault A, Almouzni G (Nov 1998). "Recruitment of phosphorylated chromatin assembly factor 1 to chromatin after UV irradiation of human cells". The Journal of Cell Biology 143 (3): 563–75. doi:10.1083/jcb.143.3.563. PMC 2148138. PMID 9813080.
• Hattori M, Fujiyama A, Taylor TD, Watanabe H, Yada T, Park HS, Toyoda A, Ishii K, Totoki Y, Choi DK, Groner Y, Soeda E, Ohki M, Takagi T, Sakaki Y, Taudien S, Blechschmidt K, Polley A, Menzel U, Delabar J, Kumpf K, Lehmann R, Patterson D, Reichwald K, Rump A, Schillhabel M, Schudy A, Zimmermann W, Rosenthal A, Kudoh J, Schibuya K, Kawasaki K, Asakawa S, Shintani A, Sasaki T, Nagamine K, Mitsuyama S, Antonarakis SE, Minoshima S, Shimizu N, Nordsiek G, Hornischer K, Brant P, Scharfe M, Schon O, Desario A, Reichelt J, Kauer G, Blocker H, Ramser J, Beck A, Klages S, Hennig S, Riesselmann L, Dagand E, Haaf T, Wehrmeyer S, Borzym K, Gardiner K, Nizetic D, Francis F, Lehrach H, Reinhardt R, Yaspo ML (May 2000). "The DNA sequence of human chromosome 21". Nature 405 (6784): 311–9. doi:10.1038/35012518. PMID 10830953.
• Keller C, Krude T (Nov 2000). "Requirement of Cyclin/Cdk2 and protein phosphatase 1 activity for chromatin assembly factor 1-dependent chromatin assembly during DNA synthesis". The Journal of Biological Chemistry 275 (45): 35512–21. doi:10.1074/jbc.M003073200. PMID 10938080.
• Mello JA, Silljé HH, Roche DM, Kirschner DB, Nigg EA, Almouzni G (Apr 2002). "Human Asf1 and CAF-1 interact and synergize in a repair-coupled nucleosome assembly pathway". EMBO Reports 3 (4): 329–34. doi:10.1093/embo-reports/kvf068. PMC 1084056. PMID 11897662.
• Morel AP, Sentis S, Bianchin C, Le Romancer M, Jonard L, Rostan MC, Rimokh R, Corbo L (Jul 2003). "BTG2 antiproliferative protein interacts with the human CCR4 complex existing in vivo in three cell-cycle-regulated forms". Journal of Cell Science 116 (Pt 14): 2929–36. doi:10.1242/jcs.00480. PMID 12771185.
• Nabatiyan A, Krude T (Apr 2004). "Silencing of chromatin assembly factor 1 in human cells leads to cell death and loss of chromatin assembly during DNA synthesis". Molecular and Cellular Biology 24 (7): 2853–62. doi:10.1128/MCB.24.7.2853-2862.2004. PMC 371118. PMID 15024074.
• Shim KS, Bergelson JM, Furuse M, Ovod V, Krude T, Lubec G (2004). "Reduction of chromatin assembly factor 1 p60 and C21orf2 protein, encoded on chromosome 21, in Down syndrome brain". Journal of Neural Transmission. Supplementum (67): 117–28. doi:10.1007/978-3-7091-6721-2_10. PMID 15068244.
• Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP (Aug 2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the United States of America 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
• Lin KT, Lu RM, Tarn WY (Oct 2004). "The WW domain-containing proteins interact with the early spliceosome and participate in pre-mRNA splicing in vivo". Molecular and Cellular Biology 24 (20): 9176–85. doi:10.1128/MCB.24.20.9176-9185.2004. PMC 517884. PMID 15456888.
• Groth A, Ray-Gallet D, Quivy JP, Lukas J, Bartek J, Almouzni G (Jan 2005). "Human Asf1 regulates the flow of S phase histones during replicational stress". Molecular Cell 17 (2): 301–11. doi:10.1016/j.molcel.2004.12.018. PMID 15664198.