CLASP1

Cytoplasmic linker associated protein 1
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 4K92​
Identifiers
Symbols	CLASP1 ; MAST1
External IDs	OMIM: 605852 MGI: 1923957 HomoloGene: 41024 GeneCards: CLASP1 Gene
Gene ontology Molecular function • dystroglycan binding • protein binding • microtubule binding • kinetochore binding • microtubule plus-end binding Cellular component • kinetochore • condensed chromosome kinetochore • Golgi apparatus • centrosome • kinetochore microtubule • cytosol • spindle microtubule • cytoplasmic microtubule • focal adhesion • cell cortex • membrane • cortical microtubule cytoskeleton • centrosomal corona • basal cortex • extracellular exosome Biological process • G2/M transition of mitotic cell cycle • microtubule cytoskeleton organization • mitotic cell cycle • microtubule bundle formation • vesicle targeting • organelle organization • microtubule nucleation • negative regulation of microtubule depolymerization • Golgi organization • mitotic spindle organization • establishment or maintenance of cell polarity • small GTPase mediated signal transduction • axon guidance • exit from mitosis • regulation of gastrulation • positive regulation of epithelial cell migration • regulation of epithelial to mesenchymal transition • microtubule organizing center organization • negative regulation of microtubule polymerization or depolymerization • microtubule anchoring • positive regulation of exocytosis • establishment of spindle orientation • cell division • negative regulation of stress fiber assembly • regulation of focal adhesion assembly • regulation of microtubule cytoskeleton organization • positive regulation of extracellular matrix disassembly • establishment of epithelial cell polarity • negative regulation of wound healing, spreading of epidermal cells • positive regulation of basement membrane assembly involved in embryonic body morphogenesis Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	23332	76707
Ensembl	ENSG00000074054	ENSMUSG00000064302
UniProt	Q7Z460	Q80TV8
RefSeq (mRNA)	NM_001142273	NM_001081276
RefSeq (protein)	NP_001135745	NP_001280229
Location (UCSC)	Chr 2: 121.34 – 121.65 Mb	Chr 1: 118.39 – 118.61 Mb
PubMed search

Cytoplasmic linker associated protein 1, also known as CLASP1, is a protein which in humans is encoded by the CLASP1 gene.^[1]

Function

CLASP1 belongs to a family of microtubule-associated proteins involved in attachment of microtubules to the cell cortex in animals^[2] and plants.^[3] CLASPs, such as CLASP1, interact with CLIPs (e.g., CLIP1). In animal cells, CLASP1 is involved in the regulation of microtubule dynamics at the kinetochore and throughout the spindle.^[1][4] CLASP1 controls the interactions of astral microtubules with the cell cortex in mitosis, which is important for the proper positioning and orientation of the spindle. ^[5]

References

1. "Entrez Gene: CLASP1 cytoplasmic linker associated protein 1".
2. Lansbergen G, Grigoriev I, Mimori-Kiyosue Y; et al. (2006). "CLASPs attach microtubule plus ends to the cell cortex through a complex with LL5beta". Developmental Cell 11 (1): 21–32. doi:10.1016/j.devcel.2006.05.012. PMID 16824950.
3. Ambrose JC, Wasteneys GO (2008). "CLASP Modulates Microtubule-Cortex Interaction during Self-Organization of Acentrosomal Microtubules". Molecular Biology of the Cell 19 (11): 4730–4737. doi:10.1091/mbc.E08-06-0665. PMC 2575154. PMID 18716054.
4. Maiato H, Fairley EA, Rieder CL, Swedlow JR, Sunkel CE, Earnshaw WC (June 2003). "Human CLASP1 is an outer kinetochore component that regulates spindle microtubule dynamics". Cell 113 (7): 891–904. doi:10.1016/S0092-8674(03)00465-3. PMID 12837247.
5. Samora, Catarina P.; Mogessie, Binyam; Conway, Leslie; Ross, Jennifer L.; Straube, Anne; McAinsh, Andrew D. (7 August 2011). "MAP4 and CLASP1 operate as a safety mechanism to maintain a stable spindle position in mitosis". Nature Cell Biology 13 (9): 1040–1050. doi:10.1038/ncb2297. PMID 21822276.

Further reading

• Ambrose JC, Wasteneys GO (2008). "CLASP Modulates Microtubule-Cortex Interaction during Self-Organization of Acentrosomal Microtubules". Mol Biol Cell 19 (11): 4730–4737. doi:10.1091/mbc.E08-06-0665. PMC 2575154. PMID 18716054.
• Galjart N (2005). "CLIPs and CLASPs and cellular dynamics". Nat. Rev. Mol. Cell Biol. 6 (6): 487–98. doi:10.1038/nrm1664. PMID 15928712.
• Ishikawa K, Nagase T, Suyama M; et al. (1998). "Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Res. 5 (3): 169–76. doi:10.1093/dnares/5.3.169. PMID 9734811.
• Lemos CL, Sampaio P, Maiato H; et al. (2000). "Mast, a conserved microtubule-associated protein required for bipolar mitotic spindle organization". EMBO J. 19 (14): 3668–82. doi:10.1093/emboj/19.14.3668. PMC 313969. PMID 10899121.
• Akhmanova A, Hoogenraad CC, Drabek K; et al. (2001). "Clasps are CLIP-115 and -170 associating proteins involved in the regional regulation of microtubule dynamics in motile fibroblasts". Cell 104 (6): 923–35. doi:10.1016/S0092-8674(01)00288-4. PMID 11290329.
• Strausberg RL, Feingold EA, Grouse LH; et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Maiato H, Fairley EA, Rieder CL; et al. (2003). "Human CLASP1 is an outer kinetochore component that regulates spindle microtubule dynamics". Cell 113 (7): 891–904. doi:10.1016/S0092-8674(03)00465-3. PMID 12837247.
• Maiato H, Rieder CL, Earnshaw WC, Sunkel CE (2004). "How do kinetochores CLASP dynamic microtubules?". Cell Cycle 2 (6): 511–4. doi:10.4161/cc.2.6.576. PMID 14504462.
• Ota T, Suzuki Y, Nishikawa T; et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Beausoleil SA, Jedrychowski M, Schwartz D; et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
• Jin J, Smith FD, Stark C; et al. (2004). "Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization". Curr. Biol. 14 (16): 1436–50. doi:10.1016/j.cub.2004.07.051. PMID 15324660.
• Gerhard DS, Wagner L, Feingold EA; et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Mimori-Kiyosue Y, Grigoriev I, Lansbergen G; et al. (2005). "CLASP1 and CLASP2 bind to EB1 and regulate microtubule plus-end dynamics at the cell cortex". J. Cell Biol. 168 (1): 141–53. doi:10.1083/jcb.200405094. PMC 2171674. PMID 15631994.
• Hillier LW, Graves TA, Fulton RS; et al. (2005). "Generation and annotation of the DNA sequences of human chromosomes 2 and 4". Nature 434 (7034): 724–31. doi:10.1038/nature03466. PMID 15815621.
• Venables JP, Bourgeois CF, Dalgliesh C; et al. (2005). "Up-regulation of the ubiquitous alternative splicing factor Tra2beta causes inclusion of a germ cell-specific exon". Hum. Mol. Genet. 14 (16): 2289–303. doi:10.1093/hmg/ddi233. PMID 16000324.
• Aonuma M, Miyamoto M, Inoue YH; et al. (2006). "Microtubule bundle formation and cell death induced by the human CLASP/Orbit N-terminal fragment". Cell Struct. Funct. 30 (1): 7–13. doi:10.1247/csf.30.7. PMID 16145243.
• Lansbergen G, Grigoriev I, Mimori-Kiyosue Y; et al. (2006). "CLASPs attach microtubule plus ends to the cell cortex through a complex with LL5beta". Dev. Cell 11 (1): 21–32. doi:10.1016/j.devcel.2006.05.012. PMID 16824950.
• Mimori-Kiyosue Y, Grigoriev I, Sasaki H; et al. (2006). "Mammalian CLASPs are required for mitotic spindle organization and kinetochore alignment". Genes Cells 11 (8): 845–57. doi:10.1111/j.1365-2443.2006.00990.x. PMID 16866869.
• Pereira AL, Pereira AJ, Maia AR; et al. (2007). "Mammalian CLASP1 and CLASP2 Cooperate to Ensure Mitotic Fidelity by Regulating Spindle and Kinetochore Function". Mol. Biol. Cell 17 (10): 4526–42. doi:10.1091/mbc.E06-07-0579. PMC 1635371. PMID 16914514.
• Ewing RM, Chu P, Elisma F; et al. (2007). "Large-scale mapping of human protein–protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.