CSTF2

Cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa
PDB rendering based on 1p1t.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1P1T​, 2J8P​
Identifiers
Symbols	CSTF2 ; CstF-64
External IDs	OMIM: 300907 MGI: 1343054 HomoloGene: 80171 GeneCards: CSTF2 Gene
Gene ontology Molecular function • nucleotide binding • RNA binding • protein binding • poly(A) RNA binding Cellular component • nucleus • nucleoplasm • mRNA cleavage and polyadenylation specificity factor complex • cleavage body Biological process • mRNA splicing, via spliceosome • transcription from RNA polymerase II promoter • termination of RNA polymerase II transcription • mRNA polyadenylation • mRNA cleavage • RNA splicing • gene expression • mRNA 3'-end processing Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	1478	108062
Ensembl	ENSG00000101811	ENSMUSG00000031256
UniProt	P33240	Q8BIQ5
RefSeq (mRNA)	NM_001306206	NM_001290398
RefSeq (protein)	NP_001293135	NP_001277327
Location (UCSC)	Chr X: 100.82 – 100.84 Mb	Chr X: 134.06 – 134.09 Mb
PubMed search

Cleavage stimulation factor 64 kDa subunit is a protein that in humans is encoded by the CSTF2 gene.^[1][2]

This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs.^[2]

Interactions

CSTF2 has been shown to interact with CSTF3,^[3] SUB1,^[4] SYMPK,^[3] BARD1^[5][6] and BRCA1.^[5][6]

References

1. Takagaki Y, MacDonald CC, Shenk T, Manley JL (March 1992). "The human 64-kDa polyadenylylation factor contains a ribonucleoprotein-type RNA binding domain and unusual auxiliary motifs". Proc Natl Acad Sci U S A 89 (4): 1403–7. doi:10.1073/pnas.89.4.1403. PMC 48459. PMID 1741396.
2. "Entrez Gene: CSTF2 cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa".
3. Takagaki, Y; Manley J L (March 2000). "Complex protein interactions within the human polyadenylation machinery identify a novel component". Mol. Cell. Biol. (UNITED STATES) 20 (5): 1515–25. doi:10.1128/MCB.20.5.1515-1525.2000. ISSN 0270-7306. PMC 85326. PMID 10669729.
4. Calvo, O; Manley J L (May 2001). "Evolutionarily conserved interaction between CstF-64 and PC4 links transcription, polyadenylation, and termination". Mol. Cell (United States) 7 (5): 1013–23. doi:10.1016/S1097-2765(01)00236-2. ISSN 1097-2765. PMID 11389848.
5. Kleiman, F E; Manley J L (March 2001). "The BARD1-CstF-50 interaction links mRNA 3' end formation to DNA damage and tumor suppression". Cell (United States) 104 (5): 743–53. doi:10.1016/S0092-8674(01)00270-7. ISSN 0092-8674. PMID 11257228.
6. Kleiman, F E; Manley J L (September 1999). "Functional interaction of BRCA1-associated BARD1 with polyadenylation factor CstF-50". Science (UNITED STATES) 285 (5433): 1576–9. doi:10.1126/science.285.5433.1576. ISSN 0036-8075. PMID 10477523.

Further reading

• Takagaki Y, Manley JL (1992). "A human polyadenylation factor is a G protein beta-subunit homologue.". J. Biol. Chem. 267 (33): 23471–4. PMID 1358884.
• Murthy KG, Manley JL (1995). "The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'-end formation.". Genes Dev. 9 (21): 2672–83. doi:10.1101/gad.9.21.2672. PMID 7590244.
• Takagaki Y, Manley JL (1994). "A polyadenylation factor subunit is the human homologue of the Drosophila suppressor of forked protein.". Nature 372 (6505): 471–4. doi:10.1038/372471a0. PMID 7984242.
• Rüegsegger U, Beyer K, Keller W (1996). "Purification and characterization of human cleavage factor Im involved in the 3' end processing of messenger RNA precursors.". J. Biol. Chem. 271 (11): 6107–13. doi:10.1074/jbc.271.11.6107. PMID 8626397.
• Schul W, Groenhout B, Koberna K; et al. (1996). "The RNA 3' cleavage factors CstF 64 kDa and CPSF 100 kDa are concentrated in nuclear domains closely associated with coiled bodies and newly synthesized RNA.". EMBO J. 15 (11): 2883–92. PMC 450226. PMID 8654386.
• Takagaki Y, Seipelt RL, Peterson ML, Manley JL (1997). "The polyadenylation factor CstF-64 regulates alternative processing of IgM heavy chain pre-mRNA during B cell differentiation.". Cell 87 (5): 941–52. doi:10.1016/S0092-8674(00)82000-0. PMID 8945520.
• Takagaki Y, Manley JL (1997). "RNA recognition by the human polyadenylation factor CstF.". Mol. Cell. Biol. 17 (7): 3907–14. PMC 232243. PMID 9199325.
• Martincic K, Campbell R, Edwalds-Gilbert G; et al. (1998). "Increase in the 64-kDa subunit of the polyadenylation/cleavage stimulatory factor during the G0 to S phase transition.". Proc. Natl. Acad. Sci. U.S.A. 95 (19): 11095–100. doi:10.1073/pnas.95.19.11095. PMC 21601. PMID 9736695.
• Takagaki Y, Manley JL (1999). "Levels of polyadenylation factor CstF-64 control IgM heavy chain mRNA accumulation and other events associated with B cell differentiation.". Mol. Cell 2 (6): 761–71. doi:10.1016/S1097-2765(00)80291-9. PMID 9885564.
• Kleiman FE, Manley JL (1999). "Functional interaction of BRCA1-associated BARD1 with polyadenylation factor CstF-50.". Science 285 (5433): 1576–9. doi:10.1126/science.285.5433.1576. PMID 10477523.
• Takagaki Y, Manley JL (2000). "Complex protein interactions within the human polyadenylation machinery identify a novel component.". Mol. Cell. Biol. 20 (5): 1515–25. doi:10.1128/MCB.20.5.1515-1525.2000. PMC 85326. PMID 10669729.
• Dass B, McMahon KW, Jenkins NA; et al. (2001). "The gene for a variant form of the polyadenylation protein CstF-64 is on chromosome 19 and is expressed in pachytene spermatocytes in mice.". J. Biol. Chem. 276 (11): 8044–50. doi:10.1074/jbc.M009091200. PMID 11113135.
• Kleiman FE, Manley JL (2001). "The BARD1-CstF-50 interaction links mRNA 3' end formation to DNA damage and tumor suppression.". Cell 104 (5): 743–53. doi:10.1016/S0092-8674(01)00270-7. PMID 11257228.
• Calvo O, Manley JL (2001). "Evolutionarily conserved interaction between CstF-64 and PC4 links transcription, polyadenylation, and termination.". Mol. Cell 7 (5): 1013–23. doi:10.1016/S1097-2765(01)00236-2. PMID 11389848.
• Bléoo S, Sun X, Hendzel MJ; et al. (2002). "Association of human DEAD box protein DDX1 with a cleavage stimulation factor involved in 3'-end processing of pre-MRNA.". Mol. Biol. Cell 12 (10): 3046–59. doi:10.1091/mbc.12.10.3046. PMC 60154. PMID 11598190.
• Strausberg RL, Feingold EA, Grouse LH; et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Pérez Cañadillas JM, Varani G (2003). "Recognition of GU-rich polyadenylation regulatory elements by human CstF-64 protein.". EMBO J. 22 (11): 2821–30. doi:10.1093/emboj/cdg259. PMC 156756. PMID 12773396.
• Ota T, Suzuki Y, Nishikawa T; et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs.". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Xing H, Mayhew CN, Cullen KE; et al. (2004). "HSF1 modulation of Hsp70 mRNA polyadenylation via interaction with symplekin.". J. Biol. Chem. 279 (11): 10551–5. doi:10.1074/jbc.M311719200. PMID 14707147.