CSTF3

Cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kDa
PDB rendering based on 2ond.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 2ond​, 2ooe​
Identifiers
Symbols	CSTF3 ; CSTF-77
External IDs	OMIM: 600367 MGI: 1351825 HomoloGene: 1014 GeneCards: CSTF3 Gene
Gene ontology Molecular function • RNA binding • mRNA binding • protein binding • poly(A) RNA binding Cellular component • nucleus • nucleoplasm Biological process • mRNA splicing, via spliceosome • transcription from RNA polymerase II promoter • termination of RNA polymerase II transcription • mRNA polyadenylation • mRNA cleavage • RNA splicing • gene expression • mRNA 3'-end processing Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	1479	228410
Ensembl	ENSG00000176102	ENSMUSG00000027176
UniProt	Q12996	Q99LI7
RefSeq (mRNA)	NM_001033505	NM_001037326
RefSeq (protein)	NP_001028677	NP_001032403
Location (UCSC)	Chr 11: 33.08 – 33.16 Mb	Chr 2: 104.59 – 104.67 Mb
PubMed search

Cleavage stimulation factor 77 kDa subunit is a protein that in humans is encoded by the CSTF3 gene.^[1][2]

The protein encoded by this gene is one of three (including CSTF1 and CSTF2) cleavage stimulation factors that combine to form the cleavage stimulation factor complex (CSTF). This complex is involved in the polyadenylation and 3' end cleavage of pre-mRNAs. The encoded protein functions as a homodimer and interacts directly with both CSTF1 and CSTF2 in the CSTF complex. Alternative splicing results in multiple transcript variants encoding different isoforms.^[2]

Interactions

CSTF3 has been shown to interact with CSTF2.^[3]

References

1. Takagaki Y, Manley JL (December 1994). "A polyadenylation factor subunit is the human homologue of the Drosophila suppressor of forked protein". Nature 372 (6505): 471–4. doi:10.1038/372471a0. PMID 7984242.
2. "Entrez Gene: CSTF3 cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kDa".
3. Takagaki, Y; Manley J L (March 2000). "Complex protein interactions within the human polyadenylation machinery identify a novel component". Mol. Cell. Biol. (UNITED STATES) 20 (5): 1515–25. doi:10.1128/MCB.20.5.1515-1525.2000. ISSN 0270-7306. PMC 85326. PMID 10669729.

Further reading

• Takagaki Y, Manley JL (1992). "A human polyadenylation factor is a G protein beta-subunit homologue.". J. Biol. Chem. 267 (33): 23471–4. PMID 1358884.
• Takagaki Y, MacDonald CC, Shenk T, Manley JL (1992). "The human 64-kDa polyadenylylation factor contains a ribonucleoprotein-type RNA binding domain and unusual auxiliary motifs.". Proc. Natl. Acad. Sci. U.S.A. 89 (4): 1403–7. doi:10.1073/pnas.89.4.1403. PMC 48459. PMID 1741396.
• Murthy KG, Manley JL (1995). "The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'-end formation.". Genes Dev. 9 (21): 2672–83. doi:10.1101/gad.9.21.2672. PMID 7590244.
• Takagaki Y, Manley JL (2000). "Complex protein interactions within the human polyadenylation machinery identify a novel component.". Mol. Cell. Biol. 20 (5): 1515–25. doi:10.1128/MCB.20.5.1515-1525.2000. PMC 85326. PMID 10669729.
• Emili A, Schieltz DM, Yates JR, Hartwell LH (2001). "Dynamic interaction of DNA damage checkpoint protein Rad53 with chromatin assembly factor Asf1.". Mol. Cell 7 (1): 13–20. doi:10.1016/S1097-2765(01)00150-2. PMID 11172707.
• Benoit B, Juge F, Iral F; et al. (2002). "Chimeric human CstF-77/Drosophila Suppressor of forked proteins rescue suppressor of forked mutant lethality and mRNA 3' end processing in Drosophila.". Proc. Natl. Acad. Sci. U.S.A. 99 (16): 10593–8. doi:10.1073/pnas.162191899. PMC 124984. PMID 12149458.
• Strausberg RL, Feingold EA, Grouse LH; et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Kaufmann I, Martin G, Friedlein A; et al. (2005). "Human Fip1 is a subunit of CPSF that binds to U-rich RNA elements and stimulates poly(A) polymerase.". EMBO J. 23 (3): 616–26. doi:10.1038/sj.emboj.7600070. PMC 1271804. PMID 14749727.
• Gerhard DS, Wagner L, Feingold EA; et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Kim J, Bhinge AA, Morgan XC, Iyer VR (2005). "Mapping DNA-protein interactions in large genomes by sequence tag analysis of genomic enrichment.". Nat. Methods 2 (1): 47–53. doi:10.1038/nmeth726. PMID 15782160.
• Rual JF, Venkatesan K, Hao T; et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network.". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
• Taylor TD, Noguchi H, Totoki Y; et al. (2006). "Human chromosome 11 DNA sequence and analysis including novel gene identification.". Nature 440 (7083): 497–500. doi:10.1038/nature04632. PMID 16554811.
• Olsen JV, Blagoev B, Gnad F; et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.". Cell 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983.
• Ewing RM, Chu P, Elisma F; et al. (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry.". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.