Epi-isozizaene 5-monooxygenase

Epi-isozizaene 5-monooxygenase
Identifiers
EC number	1.14.13.106
CAS number	1207718-51-1
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Epi-isozizaene 5-monooxygenase (EC 1.14.13.106, CYP170A1) is an enzyme with systematic name (+)-epi-isozizaene,NADPH:oxygen oxidoreductase (5-hydroxylating).^[1] This enzyme catalyses the following chemical reaction

(+)-epi-isozizaene + 2 NADPH + 2 H⁺ + 2 O₂ $\rightleftharpoons$ albaflavenone + 2 NADP⁺ + 3 H₂O (overall reaction)

(1a) (+)-epi-isozizaene + NADPH + H⁺ + O₂ $\rightleftharpoons$ (5S)-albaflavenol + NADP⁺ + H₂O

(1b) (5S)-albaflavenol + NADPH + H⁺ + O₂ $\rightleftharpoons$ albaflavenone + NADP⁺ + 2 H₂O

(2a) (+)-epi-isozizaene + NADPH + H⁺ + O₂ $\rightleftharpoons$ (5R)-albaflavenol + NADP⁺ + H₂O

(2b) (5R)-albaflavenol + NADPH + H⁺ + O₂ $\rightleftharpoons$ albaflavenone + NADP⁺ + 2 H₂O

This cytochrome P450 enzyme is purifid from bacterium Streptomyces coelicolor.

References

1. Zhao, B., Lin, X., Lei, L., Lamb, D.C., Kelly, S.L., Waterman, M.R. and Cane, D.E. (2008). "Biosynthesis of the sesquiterpene antibiotic albaflavenone in Streptomyces coelicolor A3(2)". J. Biol. Chem. 283 (13): 8183–8189. doi:10.1074/jbc.M710421200. PMC 2276382. PMID 18234666.

External links

• Epi-isozizaene 5-monooxygenase at the US National Library of Medicine Medical Subject Headings (MeSH)