Cholest-4-en-3-one 26-monooxygenase

Cholest-4-en-3-one 26-monooxygenase (EC 1.14.13.141, CYP125, CYP125A1, cholest-4-en-3-one 27-monooxygenase) is an enzyme with systematic name cholest-4-en-3-one,NADH:oxygen oxidoreductase (26-hydroxylating).^[1]^[2]^[3]^[4] This enzyme catalyses the following chemical reaction

cholest-4-en-3-one + NADH + H⁺ + O₂

\rightleftharpoons

26-hydroxycholest-4-en-3-one + NAD⁺ + H₂O

Cholest-4-en-3-one 26-monooxygenase is a heme thiolate (P450) enzyme.

References

↑ Rosloniec, K.Z., Wilbrink, M.H., Capyk, J.K., Mohn, W.W., Ostendorf, M., van der Geize, R., Dijkhuizen, L. and Eltis, L.D. (2009). "Cytochrome P450 125 (CYP125) catalyses C²⁶-hydroxylation to initiate sterol side-chain degradation in Rhodococcus jostii RHA1". Mol. Microbiol. 74 (5): 1031–1043. doi:10.1111/j.1365-2958.2009.06915.x. PMID 19843222.
↑ McLean, K.J., Lafite, P., Levy, C., Cheesman, M.R., Mast, N., Pikuleva, I.A., Leys, D. and Munro, A.W. (2009). "The Structure of Mycobacterium tuberculosis CYP125: molecular basis for cholesterol binding in a P450 needed for host infection". J. Biol. Chem. 284 (51): 35524–35533. doi:10.1074/jbc.M109.032706. PMC 2790982. PMID 19846552.
↑ Capyk, J.K., Kalscheuer, R., Stewart, G.R., Liu, J., Kwon, H., Zhao, R., Okamoto, S., Jacobs, W.R., Jr., Eltis, L.D. and Mohn, W.W. (2009). "Mycobacterial cytochrome P450 125 (cyp125) catalyzes the terminal hydroxylation of C₂₇ steroids". J. Biol. Chem. 284 (51): 35534–35542. doi:10.1074/jbc.M109.072132. PMC 2790983. PMID 19846551.
↑ Ouellet, H., Guan, S., Johnston, J.B., Chow, E.D., Kells, P.M., Burlingame, A.L., Cox, J.S., Podust, L.M. and de Montellano, P.R. (2010). "Mycobacterium tuberculosis CYP125A1, a steroid C₂₇ monooxygenase that detoxifies intracellularly generated cholest-4-en-3-one". Mol. Microbiol. 77 (3): 730–742. doi:10.1111/j.1365-2958.2010.07243.x. PMC 2909382. PMID 20545858.

External links

Cholest-4-en-3-one 26-monooxygenase at the US National Library of Medicine Medical Subject Headings (MeSH)

Oxidoreductases: dioxygenases, including steroid hydroxylases (EC 1.14)

1.14.11: 2-oxoglutarate	Prolyl hydroxylase Lysyl hydroxylase

1.14.13: NADH or NADPH	Flavin-containing monooxygenase FMO1 FMO2 FMO3 FMO4 FMO5 Nitric oxide synthase NOS1 NOS2 NOS3 Cholesterol 7 alpha-hydroxylase Methane monooxygenase 3A4 Lanosterol 14 alpha-demethylase 24-hydroxycholesterol 7α-hydroxylase

1.14.14: reduced flavin or flavoprotein	19A1 2D6 2E1

1.14.15: reduced iron-sulfur protein	11B1 11B2 11A1

1.14.16: reduced pteridine (BH4 dependent)	Phenylalanine hydroxylase Tyrosine hydroxylase Tryptophan hydroxylase

1.14.17: reduced ascorbate	Dopamine beta-hydroxylase

1.14.18-19: other	Tyrosinase Stearoyl-CoA desaturase-1

1.14.99 - miscellaneous	Cyclooxygenase Heme oxygenase (HMOX1) Squalene monooxygenase 17A1 21A2

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases(list) EC2 Transferases(list) EC3 Hydrolases(list) EC4 Lyases(list) EC5 Isomerases(list) EC6 Ligases(list)

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