Clavaminate synthase

Clavaminate synthase
Identifiers
EC number	1.14.11.21
CAS number	122799-56-8
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Clavaminate synthase (EC 1.14.11.21, clavaminate synthase 2, clavaminic acid synthase) is an enzyme with systematic name deoxyamidinoproclavaminate,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating).^{[1][2][3][4][5]} This enzyme catalyses the following chemical reaction

(1) deoxyamidinoproclavaminate + 2-oxoglutarate + O₂ $\rightleftharpoons$ amidinoproclavaminate + succinate + CO₂

(2) proclavaminate + 2-oxoglutarate + O₂ $\rightleftharpoons$ dihydroclavaminate + succinate + CO₂ + H₂O

(3) dihydroclavaminate + 2-oxoglutarate + O₂ $\rightleftharpoons$ clavaminate + succinate + CO₂ + H₂O

Clavaminate synthase contains nonheme iron.

References

1. Salowe, S.P., Krol, W.J., Iwatareuyl, D. and Townsend, C.A. (1991). "Elucidation of the order of oxidations and identification of an intermediate in the multistep clavaminate synthase reaction". Biochemistry 30 (8): 2281–2292. doi:10.1021/bi00222a034. PMID 1998687.
2. Zhou, J., Gunsior, M., Bachmann, B.O., Townsend, C.A. and Solomon, E.I. (1998). "Substrate binding to the α-ketoglutarate-dependent non-heme iron enzyme clavaminate synthase 2: Coupling mechanism of oxidative decarboxylation and hydroxylation". J. Am. Chem. Soc. 120: 13539–13540. doi:10.1021/ja983534x.
3. Zhang, Z.H., Ren, J.S., Stammers, D.K., Baldwin, J.E., Harlos, K. and Schofield, C.J. (2000). "Structural origins of the selectivity of the trifunctional oxygenase clavaminic acid synthase". Nat. Struct. Biol. 7 (2): 127–133. doi:10.1038/72398. PMID 10655615.
4. Zhou, J., Kelly, W.L., Bachmann, B.O., Gunsior, M., Townsend, C.A. and Solomon, E.I. (2001). "Spectroscopic studies of substrate interactions with clavaminate synthase 2, a multifunctional α-KG-dependent non-heme iron enzyme: Correlation with mechanisms and reactivities". J. Am. Chem. Soc. 123: 7388–7398. doi:10.1021/ja004025. PMID 11472170.
5. Townsend, C.A. (2002). "New reactions in clavulanic acid biosynthesis". Curr. Opin. Chem. Biol. 6: 583–589. doi:10.1016/S1367-5931(02)00392-7. PMID 12413541.

External links

• Clavaminate synthase at the US National Library of Medicine Medical Subject Headings (MeSH)